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Enzyme treatment method of beta-lactam antibiotics and intermediate mushroom dregs thereof

A treatment method, the technology of lactamase, applied in the direction of additional food elements, animal feed, animal feed, etc., can solve the problems of difficulty in realization, unexplained antibiotic residues, large processing volume, etc., and achieve good economic benefits and thorough treatment effects , the effect of low processing cost

Pending Publication Date: 2021-12-07
HANGZHOU JUNFENG BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, trace amounts of antibiotics remaining in the bacteria residue may be enriched in animals and plants, thereby posing potential impacts and risks on ecosystems, food safety, and human health.
On August 1, 2008, my country clearly listed antibiotic residues as "hazardous waste" and prohibited antibiotic residues from being directly used as feed and fertilizer; In order to achieve the purpose of reduction and harmlessness, but the drying and incineration of bacteria residues need to consume a large amount of heat energy, so the incineration cost is high, the processing capacity is huge, and it is difficult to realize; 3) Landfill: landfill is a relatively mature However, environmental protection problems such as groundwater pollution are prominent. As people pay more attention to and demand for environmental protection, this simple treatment method is becoming more and more unsustainable.
This method is specific for the treatment of antibiotic residues in bacteria residues, but this method measures the antibiotic residues in the liquid after centrifugation of the bacteria residues, and does not explain the residues of antibiotics in solids

Method used

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  • Enzyme treatment method of beta-lactam antibiotics and intermediate mushroom dregs thereof
  • Enzyme treatment method of beta-lactam antibiotics and intermediate mushroom dregs thereof
  • Enzyme treatment method of beta-lactam antibiotics and intermediate mushroom dregs thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment one penicillin enzyme treatment penicillin scum

[0043] Take 1KG of penicillin residue, add 1000mL of water and stir evenly, preheat at 37°C, add 20 million units / ml of penicillinase to make the final concentration of 1000 units / ml, centrifuge at 37°C for 8 hours, take the supernatant through 0.22μm HPLC detection of residual penicillin in the filtrate after microporous membrane filtration, RP-HPLC analysis method: C18 column, mobile phase A: 0.1% TFA-H2O, mobile phase B: 0.1% TFA-CAN, gradient 0-5min, 0% B, 5~15min, 0~20B, 15~20min 20%~70%B. The decomposition results of the penicillin reference substance are shown in figure 1 . For the analysis results of the supernatant of the bacteria residue, see figure 2 , Penicillin has been completely decomposed and cannot be detected. Take the bacteria residue and centrifuge and sonicate, output power 100w, 70%, 15min, centrifuge after sonication to get the supernatant, filter through a 0.22μm microporous membra...

Embodiment 2

[0044] Embodiment two wall cracking enzyme and penicillinase treatment penicillin scum

[0045] Take 1KG of penicillin residue, add 1000mL of water and stir evenly, preheat and keep warm at 37°C, add 2g of wall-splitting enzyme, including 1g of lysozyme, 0.5g of cellulase, and 0.5g of xylanase, mix well and then heat-preserve at 37°C for 8 hours , then add 20 million units / ml of penicillinase to make the final concentration of 1000 units / ml, continue to incubate at 37°C for 8 hours and centrifuge, take the bacterial residue and centrifugal sedimentation for ultrasonic treatment, output power 100w, 70%, 15min, centrifuge after ultrasonication The supernatant was filtered through a 0.22 μm microporous membrane, and the residual penicillin in the filtrate was detected by HPLC. The results are shown in Figure 4 , undetectable penicillin residues.

Embodiment 3

[0046] Example 3 Wall-splitting enzyme and penicillinase treatment of 6-APA bacteria residue

[0047] Take 1KG of 6-APA fungus residue, add 1000mL of water and stir evenly, preheat at 37°C and keep warm, add 1g of wall cracking enzyme, including 0.4g of lysozyme, 0.3g of cellulase, and 0.3g of xylanase, mix well and keep warm at 37°C Treat for 12 hours, then add 20 million units / ml of penicillinase to make the final concentration 500 units / ml, continue to incubate at 37°C for 10 hours and centrifuge, take the bacteria residue and centrifugal sedimentation for ultrasonic treatment, output power 100w, 70%, 15min, ultrasonic Afterwards, the supernatant was obtained by centrifugation, and the residual 6-APA in the filtrate was detected by HPLC after filtration through a 0.22 μm microporous membrane, and no penicillin residue was detected as a result.

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Abstract

The invention belongs to the technical field of antibiotic mushroom dreg treatment, and particularly relates to a treatment method of beta-lactam antibiotics and intermediate mushroom dregs thereof. The treatment method comprises the following steps of adding wall splitting enzymes, performing heat preservation treatment, uniformly mixing beta-lactamase with the mushroom dregs in proportion, performing heat preservation treatment under the optimal action condition of the beta-lactamase, and finally, drying and crushing wet mushroom dregs. The whole treatment process is mild in condition, low in energy consumption and low in treatment cost. In the treatment process, a large quantity of chemical reagents are not added, and the treated dry mushroom dregs do not have antibiotic medicine residues, can be used as a protein additive of animal feeds, can realize resource reuse, and have favorable economic benefits, environmental benefits and social benefits.

Description

technical field [0001] The invention belongs to the technical field of antibiotic residue treatment, and in particular relates to a method for treating β-lactam antibiotics and intermediate bacteria residue. Background technique [0002] Since Alexander Fleming invented penicillin in 1928, the ability of human beings to resist bacterial infection has been greatly improved. Penicillin has not only saved countless lives, but also led to the development of the antibiotic industry. However, with the large-scale use and misuse of antibiotics, more and more bacteria have developed resistance. For example, when penicillin G was first used, only 8% of grapes were resistant to it. By 1962, drug-resistant staph species had increased to 70%. The way to overcome bacterial resistance is to develop and use new semi-synthetic antibiotics, which have fewer side effects, lower toxicity, greater selectivity for pathogenic microorganisms, and a wide antibacterial spectrum. [0003] The disco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/14A23K20/147
CPCA23K10/14A23K20/147
Inventor 叶楠翁寿宝郑妮宁
Owner HANGZHOU JUNFENG BIOENG
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