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Microorganism soil layer dispersion mixed inoculation method

A soil layer and microbial technology, applied in the field of soil remediation, can solve the problems of long duration of microbial remediation and low remediation efficiency, and achieve the effect of easy in-situ repair, convenient operation and improved removal

Pending Publication Date: 2021-11-26
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial remediation technology has certain limitations, such as the long duration of microbial remediation and low remediation efficiency.

Method used

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  • Microorganism soil layer dispersion mixed inoculation method

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Experimental program
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Effect test

Embodiment approach

[0089] According to a preferred embodiment of the present invention, the fungus is an AM fungus (arbuscular mycorrhizal fungus), preferably selected from one or more of Glomus mosei, Glomus intraradices, and Glomus tertiformis .

[0090] Among them, AM fungi are a class of ubiquitous soil fungi that can form symbionts with some terrestrial plants.

[0091] In a further preferred embodiment, the fungus is Glomus moesei and / or Glomus intraradices, preferably Glomus moesei and Glomus intraradices;

[0092] Preferably, the weight ratio of Glomus mosei and Glomus intrarhizoi is (1-3):1, preferably (1.5-2.5):1.

[0093] The inventors of the present invention have found that inoculating the above-mentioned types and ratios of fungal agents in the soil is beneficial to work together with plants to efficiently restore heavy metal-contaminated soil.

[0094] Wherein, the AM fungus can be obtained commercially, or can be obtained by self-cultivation by conventional cultivation methods....

Embodiment 1

[0114] Follow the steps below to remediate cadmium and arsenic contaminated soil:

[0115] (1) Plow the soil to be repaired, maintain it for 30 days under natural conditions, and then apply a soil improver according to the weight ratio of the soil improver to the soil at 7:100. The soil improver is based on iron-rich biochar, kaolin and humic acid The weight ratio of phytic acid is obtained by mixing 20:30:35;

[0116] Wherein, iron-rich biochar is prepared according to the following steps:

[0117] Cultivate cattails in red soil areas, add vermicompost according to the volume ratio of vermicompost and cultivation soil as 55:100 during the cultivation process; pull out the whole cattail plant when harvesting, wash with deionized water, soak in 0.01M dilute hydrochloric acid for 1 hours, dried and pulverized at 70.0°C; in a tubular muffle furnace, heated up to 685°C at a heating rate of 4°C / min for pyrolysis, kept for 75min, and then cooled to room temperature to obtain iron-r...

Embodiment 2

[0125] Follow the steps below to remediate cadmium and arsenic contaminated soil:

[0126] (1) Plow the soil to be repaired, maintain it for 30 days under natural conditions, and then apply a soil improver according to the weight ratio of the soil improver and soil at 4:100. The soil improver is based on iron-rich biochar, kaolin and humic acid The weight ratio of phytic acid is obtained by mixing 20:35:40;

[0127] Wherein, iron-rich biochar is prepared according to the following steps:

[0128] Cultivate cattail in red soil area, add vermicompost according to the volume ratio of vermicompost and cultivation soil in the cultivation process as 60:100; pull out the whole cattail plant when harvesting, wash with deionized water, soak in 0.01M dilute hydrochloric acid for 1 hours, dried and pulverized at 70.0°C; in a tubular muffle furnace, heated up to 715°C at a heating rate of 3°C / min for pyrolysis, kept for 65 minutes, and then cooled to room temperature to obtain iron-rich ...

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Abstract

The invention discloses a microbial soil layer dispersion mixed inoculation method. The method comprises the steps of pretreating soil and inoculating inoculants in the soil. A bacterial agent and a fungal agent are sequentially applied, so that the removal of heavy metals in the soil is facilitated. The invention further discloses a remediation method of heavy metal contaminated soil. According to the method, after the soil is subjected to dispersion mixing and inoculation of microorganisms, remediation plants are planted, in-situ remediation of the soil is facilitated, cost is low, and applicability is high.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to a method for dispersing and mixing inoculation of microbial soil layers to remediate heavy metal polluted soil. Background technique [0002] Soil heavy metal pollution refers to the excess of various heavy metal elements in the soil, exceeding the limit value that the soil can bear. Excessive heavy metals have a great impact on the self-circulation capacity of the soil. According to statistics, the current polluted soil area in my country has reached 50 million mu, and soil heavy metal pollution mainly includes mercury (Hg), cadmium (Cd), lead (Pb), chromium (Cr) and metalloid arsenic (As) with significant biological toxicity. Elements such as zinc (Zn), copper (Cu), nickel (Ni) and other elements with certain toxicity. Soil heavy metal pollution has the characteristics of poor mobility of pollutants in the soil, long residence time, and inability to be degraded by micro...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/08B09C1/00
CPCB09C1/105B09C1/08B09C1/00B09C2101/00
Inventor 王雷席北斗张众磊李翔李彤彤王金生闫政王杨杨杨茹月徐剑锋刘慧
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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