Application of adipose-derived stem cells in localized scleroderma

A technology for localized scleroderma and fat source, applied in skin diseases, medical preparations containing active ingredients, unknown raw materials, etc., can solve the problems of tissue atrophy and adhesion, poor local blood supply, low fat survival rate, etc., to achieve The effect of reducing skin collagen content, reducing symptoms, and improving fat survival rate

Pending Publication Date: 2021-11-26
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the previous studies focused on normal fat samples, and there were few studies on patients with scleroderma. The fat transplantation of patients with localized scleroderma is different from that of normal people, even if the above technical principles of fat transplantation are followed However, the fat survival rate of patients with scleroderma is still significantly lower than that of normal people, which may be related to tissue atrophy and adhesion in localized scleroderma lesions, poor local blood supply, and local inflammatory microenvironment. Whether there are biological changes in autologous donor site fat in patients with skin diseases also needs to be verified

Method used

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  • Application of adipose-derived stem cells in localized scleroderma
  • Application of adipose-derived stem cells in localized scleroderma
  • Application of adipose-derived stem cells in localized scleroderma

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Culture and Expansion of Adipose-derived Stem Cells

[0027] Adipose tissue was obtained from the Department of Plastic Surgery, Peking Union Medical College Hospital, and the discarded adipose tissue after liposuction, and informed consent was obtained from the patients.

[0028] 1. Extraction of fat stem cells

[0029] (1) Add 20ml of fat obtained from liposuction into a 50ml centrifuge tube, add D.hangks to 40ml, and centrifuge at 800r for 3min;

[0030] (2) Suck off the liquid at the bottom, transfer the fat to a new centrifuge tube, wash D.hangks twice, and centrifuge at 800r for 3min;

[0031] (3) Add collagenase to the tube, the volume is 1 / 3-1 / 4 of the fat volume, shake at 37°C, 200rpm, 30min;

[0032] (4) After the shaker is taken out, filter with a 100nm filter (wet the filter with Dhangks, which is good for filtering);

[0033] (5) The filtrate was centrifuged at 1500r for 8min;

[0034] (6) After centrifugation, absorb the upper layer of grease...

Embodiment 3

[0051] Example 3 Adipose stem cell-assisted fat transplantation in the treatment of scleroderma nude mouse model

[0052] 1. Modeling of nude mice with bleomycin scleroderma

[0053] The bleomycin powder was diluted to 200ug / ml with phosphate buffered saline (PBS), filtered and sterilized through a 0.22um sterile filter membrane, and stored for future use. In the modeling group, bleomycin dilution was injected subcutaneously on the back of nude mice, and in the control group, PBS was injected subcutaneously on the back of nude mice, once a day, 0.1ml each time, for a total of 4 weeks, and the elasticity and appearance of the back skin were observed. General changes, 2 mice in the pre-experiment modeling group and 2 mice in the control group were killed at 4 weeks, the back skin of the injection area was taken and fixed in tissue fixative, and HE staining and Masson staining were performed respectively. Compared with the mice in the PBS control group, it was confirmed that Whe...

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Abstract

The invention discloses application of adipose-derived stem cells in preparation of products for assisting adipose transplantation in treating localized scleroderma. By constructing a bleomycin scleroderma nude mouse model and studying adipose-derived stem cell and adipose transplantation, the TGF-beta 1 content and the III type collagen content of scleroderma group mice can be reduced, and the symptoms of scleroderma are relieved.

Description

technical field [0001] The invention relates to the technical field, in particular to the application of adipose-derived stem cells in localized scleroderma. Background technique [0002] Scleroderma is a collagen disease characterized by fibrosis and hardening of the skin and tissues and organs. Those confined to the skin are called Lcalized scleroderma (LS), also known as Morphea. Accumulation with multiple internal organ systems is called systemic scleroderma (SSc). The early treatment of scleroderma is mainly aimed at the abnormalities of immunity, blood vessels and collagen, and is based on anti-inflammation, immunosuppression, immune regulation, improvement of blood circulation and reduction of fibrosis. Deformities such as skin sclerosis, subcutaneous fat atrophy, and local depressions left behind in the later stage often require plastic surgery. Plastic surgery for local depressed deformities currently includes autologous tissue grafts with vascular pedicles and au...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P17/00
CPCA61K35/28A61P17/00
Inventor 龙笑王晓军陈洁李竹君崇煜明张文超王晨羽俞楠泽夏泽楠黄久佐
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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