A method for evaluating the resistance of rice varieties to rice leaf dwarf disease based on artificial inoculation

A technique of tooth leaf dwarfing and artificial inoculation, applied in horticultural methods, rice cultivation, botanical equipment and methods, etc., to achieve the effect of accelerating the process of disease-resistant breeding

Active Publication Date: 2022-04-12
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, there is still a lack of resistance identification methods in the field that can simulate the rice leaf dwarf virus infection in the natural environment

Method used

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  • A method for evaluating the resistance of rice varieties to rice leaf dwarf disease based on artificial inoculation
  • A method for evaluating the resistance of rice varieties to rice leaf dwarf disease based on artificial inoculation

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Embodiment 1

[0044] Example 1 Identification of Resistance of Different Rice Varieties to Rice Dwarf Disease

[0045] 1. Rice varieties: Teyou 63, Youzhan 8, Nipponbare, Guangyedao, Guanghui 998

[0046] 2. Detection method of rice leaf dwarf virus:

[0047] (1) extracting the RNA of the rice leaf to be tested or the brown planthopper worm to be tested, reverse transcription to obtain cDNA, and use the cDNA as a template to carry out PCR detection;

[0048](2) The primers for PCR are: RRSV-F: 5'-GCCTTTCGTGCAATTCCC-3', RRSV-R: 5'-GCGTCATCA CCAAAGTTAGCC-3';

[0049] (3) The reaction conditions of PCR are: 50°C, 30min; 94°C, 4min, 94°C, 30s; 56°C, 30s; 72°C, 45s; 35 cycles; 72°C extension for 10min, 12°C storage;

[0050] (4) The obtained PCR product was detected by 1% agarose gel, and the samples with the expected band 895bp amplified were qualitatively positive samples, and the samples without the amplified band were negative samples.

[0051] 3. Identification of rice leaf dwarf resista...

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Abstract

The invention discloses a method for evaluating the resistance of rice varieties to rice tooth dwarf disease based on artificial inoculation, which includes collecting and raising brown planthoppers, obtaining non-toxic brown planthopper populations, preparing poison sources, preparing reference rice, feeding brown planthoppers with poison, artificial inoculation, Investigate, calculate the steps such as morbidity, the method of the present invention optimizes the feeding condition and evaluation system of the virus-transmitting medium brown planthopper, determines the key technical indicators such as artificial inoculation feeding time, inoculation seedling age, time, intensity, etc., formulates The manual inoculation identification technical rules for resistance and resistance of rice varieties to rice leaf dwarf disease have been formulated, which provides a reference for standardizing the identification and evaluation of disease resistance of rice varieties, and lays a solid foundation for further excavating disease resistance genes, accelerating the process of disease resistance breeding, and carrying out green comprehensive disease prevention and control. Base.

Description

technical field [0001] The invention relates to the technical field of plant resistance identification, in particular to a method for evaluating rice variety resistance to rice dwarf disease based on artificial inoculation. Background technique [0002] The pathogen of rice leaf stunt disease is rice leaf stunt virus (Riceragged stunt virus, RRSV), which is mainly transmitted by the migratory pest brown planthopper in the form of persistent proliferation. Rice can be infected and damaged at all growth stages. The plants are short, with high tillers, increased tillers, twisted leaf tips, dark green leaves, saw-toothed notches on leaf margins, venous swellings on leaf sheaths and leaves, etc. In serious cases, normal heading and fruiting cannot be achieved. In 1978, the disease was discovered and reported in Fujian Province of my country, followed by reports in Guangdong, Jiangxi and other places. The disease once broke out into disasters in the last century, and then tended ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01G7/06A01G22/22
CPCA01K67/033A01G7/06A01G22/22
Inventor 李战彪秦碧霞谢慧婷崔丽贤蔡健和陈锦清
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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