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Alpha1 microglobulin trace detection method

A technology of microglobulin and detection method, which is applied in the field of micro-detection of α1 microglobulin, which can solve problems such as inaccuracy, unstable samples, and low sensitivity

Active Publication Date: 2021-11-23
JILIN UNIV FIRST HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of imprecise and inaccurate detection methods caused by the low sensitivity and unstable samples of the above-mentioned existing methods, the present invention provides a new micro-quantity detection method for α1 microglobulin. The scheme is as follows:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0044] Preparation Example 1 Reagent a Preparation

[0045] Reagent aI

[0046] Reagent a includes polyethylene glycol, sodium zinc ethylenediaminetetraacetate, and a buffer;

[0047] The buffer in reagent a includes phosphate buffer.

[0048] The concentration of polyethylene glycol in reagent a is 0.09wt%, and the concentration of zinc sodium edetate is 0.05wt%.

[0049] The preparation method of the reagent a includes: adding all the substances included in the reagent a to the buffer therein, and mixing uniformly.

[0050] Reagent a II

[0051] Reagent a includes polyethylene glycol, sodium zinc ethylenediaminetetraacetate, and a buffer;

[0052] The buffer in reagent a includes MOPS buffer.

[0053] The concentration of polyethylene glycol in reagent a is 0.12wt%, and the concentration of zinc sodium edetate is 0.1wt%.

[0054] The preparation method of the reagent a includes: adding all the substances included in the reagent a to the buffer therein, and mixing unifo...

preparation example 2

[0074] Preparation Example 2 Reagent b Preparation

[0075] Reagent bI

[0076] Include polyvinylpyrrolidone, sodium chloride, buffer solution in the reagent b;

[0077] In reagent b, the concentration of polyvinylpyrrolidone is 5g / L, and the concentration of sodium chloride is 1g / L.

[0078] The buffer in reagent b includes 100mmol / L Tris-HCl buffer.

[0079] The preparation method of the reagent b includes: adding all the substances included in the reagent b to the buffer therein, and mixing uniformly.

[0080] Reagent bⅡ

[0081] Include polyvinylpyrrolidone, sodium chloride, buffer solution in the reagent b;

[0082] In reagent b, the concentration of polyvinylpyrrolidone is 25g / L, and the concentration of sodium chloride is 3g / L.

[0083] The buffer in reagent b includes 100mmol / L Tris-HCl buffer.

[0084] The preparation method of the reagent b includes: adding all the substances included in the reagent b to the buffer therein, and mixing uniformly.

[0085] Reage...

preparation example 3

[0124] Preparation Example 3 Reagent c Preparation

[0125] Reagent cⅠ

[0126] Reagent c includes polystyrene nanoparticles labeled only with α1 microglobulin monoclonal antibody, polystyrene nanoparticles labeled only with α1 microglobulin polyclonal antibody, and a buffer.

[0127] In reagent c, the average particle diameter of the polystyrene nanoparticles labeled only with the α1 microglobulin monoclonal antibody is larger than that of the polystyrene nanoparticles labeled only with the α1 microglobulin polyclonal antibody.

[0128] In reagent c, the average particle size of polystyrene nanoparticles labeled with α1 microglobulin monoclonal antibody is 350-400 nm, and the average particle size of polystyrene nanoparticles labeled with α1 microglobulin polyclonal antibody is 150-250nm.

[0129] In reagent c, only polystyrene nanoparticles labeled with α1 microglobulin monoclonal antibody, based on the mass of polystyrene nanoparticles, the concentration is 0.05% (w / v); o...

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Abstract

The invention discloses an alpha1 microglobulin trace detection method, and relates to the technical field of biological detection methods. The method comprises the following steps: mixing a reagent a with a sample, adding a reagent b, and carrying out mixed incubation; adding a reagent c for mixing, and reading an absorbance value; after timing reaction, reading the absorbance value again; calculating the difference value of the two absorbance values, making a regression equation function curve of the difference value to the concentration of the alpha1 microglobulin, and substituting the difference value of the sample to be detected into the function curve to calculate the concentration of the alpha1 microglobulin; the reagent a comprising polyethylene glycol and sodium zinc ethylene diamine tetraacetate; the reagent b comprising polyvinylpyrrolidone and sodium chloride; the reagent c comprising polystyrene nanoparticles only marked with an alpha1 microglobulin monoclonal antibody and polystyrene nanoparticles only marked with an alpha1 microglobulin polyclonal antibody. According to the method, loss of alpha1 microglobulin in the sample in the detection process is effectively reduced, detection errors are reduced through specific reagent use and proportion adjustment, and sensitivity is improved.

Description

technical field [0001] The invention relates to the technical field of biological detection methods, in particular to a micro-detection method for α1 microglobulin. Background technique [0002] α1 microglobulin is a glycoprotein synthesized by the human liver and lymphocytes with a molecular weight of about 33,000 Daltons. It consists of 167 amino acids. It has cross-reaction with antigenic determinants such as human leukocyte antigen HLA-A11, HLA-B20 and HLA-51. Since Ekstron et al. isolated α-Microglobulin (α-MG) from the urine of patients with chronic cadmium poisoning in 1975, foreign scholars have done a lot of research on the source, biochemical characteristics and clinical application value of α1-MG. Research. α1-MG is widely present in various body fluids of the human body and on the surface of lymphocyte membranes. α1-MG in blood exists in two forms, namely free α1-MG and α1-MG bound to IgA (α1MG-1gA). Normal Under normal circumstances, α1MG-1gA accounts for abou...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/01G01N33/68
CPCG01N21/31G01N21/01G01N33/68G01N2021/0112
Inventor 刘天明王旭
Owner JILIN UNIV FIRST HOSPITAL
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