Method for industrially preparing high-purity ergothioneine
An ergothioneine, high-purity technology, applied in the fields of food, medicine, and biochemical products, can solve the problems of reducing the single batch processing capacity of the HILIC column, affecting the service life of the HILIC column, increasing the usage of organic solvents, etc., and improving process stability. High performance, low cost, and the effect of increasing the single processing capacity
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Embodiment 1
[0044] 3 tons of mycelium fermentation liquid, separated by industrial centrifuge (separation factor: 800g), after the mycelium is suspended in water, the pipeline is transferred to the feed liquid tank, replenished with water to 2000L and stirred, treated at 90°C for 30 minutes, and separated by industrial centrifuge again ( Separation factor 800g), obtain 1800L feed liquid that contains ergothioneine; The feed liquid that contains ergothioneine double-effect concentrates to 200L, ammoniacal liquor adjusts concentrated solution pH to 9, pipeline filtration removes sediment after standing 30min, obtains ergothioneine Concentrated solution: The ergothioneine concentrated solution was successively filtered through a 0.1 μm ceramic membrane and a 4KD hollow fiber membrane to obtain 180 L of ergothioneine ultrafiltrate.
[0045] The ergothioneine ultrafiltrate obtained above was processed through HZ214 decolorization column, flow rate 1BV / h, washing volume 2BV, collected flow-throu...
Embodiment 2
[0050] 3 tons of mycelium fermentation broth, separated by an industrial centrifuge, after the mycelium is suspended in water, the pipeline is transferred to the feed liquid tank, replenished with water to 2000L, stirred, treated at 90°C for 30min, and separated by an industrial centrifuge again to obtain 1800L containing ergot sulfur Because of the feed liquid; the feed liquid containing ergothioneine is double-effect concentrated to 200L, the ammonia water adjusts the pH of the concentrated solution to 8.5, and after standing for 30min, the pipeline filter removes the sediment to obtain the ergothioneine concentrated solution; the ergothioneine concentrated solution passes 0.1 μm ceramic membrane and 4KD hollow fiber membrane filtration, obtain ergothioneine ultrafiltrate 200L.
[0051] The above-mentioned obtained ergothioneine ultrafiltrate was processed through HZ214 decolorization column, the flow rate was 1BV / h, the washing volume was 2BV, and the flow-through liquid was...
Embodiment 3
[0056] 3 tons of mycelium fermentation broth, separated by an industrial centrifuge, after the mycelium is suspended in water, the pipeline is transferred to the feed liquid tank, replenished with water to 2100L, stirred, treated at 90°C for 30min, and separated by an industrial centrifuge again to obtain 1800L containing ergot sulfur Because of the feed liquid; the feed liquid containing ergothioneine is double-effect concentrated to 250L, the ammonia water adjusts the pH of the concentrated solution to 9.1, and after standing for 30min, the pipeline filter removes the sediment to obtain the ergothioneine concentrated solution; the ergothioneine concentrated solution passes 0.1 μm ceramic membrane and 4KD hollow fiber membrane filtration, obtain ergothioneine ultrafiltrate 230L.
[0057] The above-mentioned obtained ergothioneine ultrafiltrate was processed through HZ214 decolorization column, the flow rate was 1BV / h, the washing volume was 2BV, and the flow-through liquid was...
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