Dry-type immunofluorescence chromatography influenza A/B virus antigen detection kit

Abstract

The invention relates to the technical field of medical detection and immunoassay, and relates to a dry-type immunofluorescence chromatography influenza A / B virus antigen detection kit, which comprises a detection card, a detection chip and a detection buffer solution, wherein the detection card comprises a reagent strip, wherein the reagent strip comprises absorbent paper, a nitrocellulose membrane, a combination pad and a sample pad; the nitrocellulose membrane is sequentially coated with a quality control line C, a detection line T1 and a detection line T2; a fluorescent antibody formed by a mouse anti-human influenza A / B virus monoclonal antibody marked by a fluorescent substance is solid-phase on the combination pad, so that the influenza A / B virus antigen can be simply, quickly and accurately detected. The influenza virus antigen in a sample is detected by adopting an immunofluorescence double-antibody sandwich method. Compared with the prior art, the kit is simpler and more convenient to operate, saves manpower and material resources, is high in sensitivity and does not need to be operated by professionals. A detection result can be directly obtained through a fluorescence immunoassay quantitative analyzer; the kit is suitable for medical units at all levels, is easy to popularize and has wide applicability.

Description

technical field [0001] The invention relates to the technical field of medical detection and immune analysis, in particular to a dry immunofluorescence chromatography type A / B influenza virus antigen detection kit. Background technique [0002] Influenza viruses are divided into three types: A, B, and C. Type A is the most threatening, followed by Type B. According to the antigenicity of hemagglutinin (HA) and neuraminidase (NA) proteins, influenza A virus can be divided into 15 H subtypes (H1-H15) and 9 N subtypes (N1-N9) . Because the nucleic acid sequence encoding HA or NA is prone to mutations, resulting in changes in the antigenic epitopes of HA or NA, this antigenic change makes the original specific immunity of the population invalid, so influenza A viruses often cause large-scale Even a worldwide flu epidemic. [0003] Influenza virus is a kind of upper respiratory tract infection virus with extremely high morbidity rate, which can reach 80-90% of the population w...

AI Technical Summary

Problems solved by technology

Among them, the RT-PCR method is the preferred diagnostic method with the highest accuracy, but its disadvantages are high detection cost, long detection time, independent requirements for different reaction conditions in the laboratory, and the need for professionals to operate through equipment, which has certain limitations. is not suitable for large-scale detection applications; the ELISA method takes into account both speed and accuracy, and is a classic method in hospital laboratory departments and disease control system laboratories; colloidal gold method is the fastest, simple and easy, and is suitable for general screening, but The sensitivity of gold detection is low, often resulting in missed detection

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