Cell sap glucose content detection method

Abstract

The invention discloses a cell sap glucose content detection method. The method comprises the following steps: a, adding a buffer agent, a cell sap sample and a standard substance into a dilution plate; b, preparing a glucose detection reaction solution; c, adding a glucose detection reaction solution into the detection plate; d, detecting the glucose concentration of the cell sap sample by a microplate reader, and calculating the glucose content of the cell sap sample, wherein the mixing machine comprises a main body, a motor, a rotating frame, a bracket, a feeding device, a rotating roller, a rotating disc and a mixing device; according to the method, glucose content detection can be completed by using a small amount of cell sap sample in the cell culture process, pretreatment work such as centrifugation is not needed, and detection steps are simplified; the determination can be completed by the microplate reader, and the operation is simple; meanwhile, the mixing device is adopted when the glucose detection reaction solution is prepared so that the mixing efficiency can be improved; and quantitative feeding can be realized by adopting the feeding device, so that the preparation effect is improved.

Description

technical field [0001] The invention belongs to the technical field of detection of glucose content in cell fluid, and in particular relates to a detection method for glucose content in cell fluid. Background technique [0002] Glucose, an organic compound with the molecular formula C6H12O6, is the most widely distributed and most important monosaccharide in nature. Glucose plays an important role in the field of biology. It is the energy source and metabolic intermediate product of living cells, and it is also the main energy supply material of cells. In cell culture, especially in batch-fed experiments, the monitoring and timely supplementation of glucose is the basis for maintaining cell survival. [0003] At present, the glucose content in laboratory cells is mainly detected by ultraviolet spectrophotometer or automatic biochemical analyzer. The detection principle is mainly based on glucose oxidase method, hexokinase method, glucose oxygen electrode method, etc. Altho...

AI Technical Summary

Problems solved by technology

Although the instrument detection is highly automated and convenient, it is difficult to complete the detection of a large number of cell fluid samples at the same time due to the limited throughput of the instrument.

In addition, the use of biochemical analyzers to detect Glucose is limited by the instrument, and supporting reagents, calibrators, and quality controls are required, and the cost per unit cell fluid sample is relatively high

Although the cost of the ultraviolet method is relatively low, it requires a relatively large amount of cell fluid samples, which is not conducive to the use of small volume cell culture.

[0004] The disadvantages of these existing technologies are: the detection cost is high, the amount of cell fluid samples is large, and the detection of a large number of cell fluid samples cannot be completed at the same time, and the use is limited by instruments, consumables, resources, etc.; The cell fluid sample can quickly, accurately and massively complete the reaction solution and detection method of glucose content detection, and the present invention solves such problems

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