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Vascularized double-layer engineering skin based on 3D printing and preparation method thereof

A 3D printing and engineering skin technology, applied in the field of bioengineering, can solve problems such as insufficient skin sources, achieve precise control of the formation of blood vessel branches, achieve precise control, and promote the effect of cell proliferation

Pending Publication Date: 2021-11-05
SUZHOU RUIHUA HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The purpose of the present invention is to promote the proliferation of epidermal stem cells and increase the thickness of the epidermis through traction, thereby constructing high-quality double-layered skin tissue and solving the problem of insufficient skin sources for clinical wound repair

Method used

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  • Vascularized double-layer engineering skin based on 3D printing and preparation method thereof
  • Vascularized double-layer engineering skin based on 3D printing and preparation method thereof
  • Vascularized double-layer engineering skin based on 3D printing and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0101]Vascularized double-layer engineered skin based on 3D printing. The vascularized double-layer engineered skin includes dermis skin tissue and epidermis skin tissue. The dermis skin tissue is obtained by 3D printing based on tension control; the epidermis skin tissue is inoculated on The surface of the dermis skin tissue.

[0102] For this vascularized double-layer engineered skin, the stretching force can be controlled by changing the distance between the 3D printed frame structures during the construction process, which can not only effectively promote the proliferation of cells in the skin tissue of the epidermis, but also precisely control the formation of blood vessel branches. Tissue-engineered skin with normal bilayer skin tissue provides effective support and expands the clinical application of tissue-engineered skin in wound repair.

Embodiment 2

[0104] Based on 3D printing, vascularized double-layer engineered skin includes dermis skin tissue and epidermis skin tissue. The dermis skin tissue is a complex of GelMA-fibrin-endothelial cells and fibroblasts controlled by 3D stretching force. Obtained by printing; the epidermis skin tissue is inoculated on the surface of the dermis skin tissue;

[0105] Endothelial cells include endothelial cells induced by adipose stem cells or human umbilical vein endothelial cells, preferably human umbilical vein endothelial cells; wherein, the fibrin concentration is 5-10 mg / ml, and can be selected as 5.5 mg / ml, 6 mg / ml, 6.5 mg / ml, 7mg / ml, 7.5mg / ml, 8mg / ml, 8.5mg / ml, 9mg / ml, 9.5mg / ml, preferably 7.5mg / ml;

[0106] The epidermal skin tissue is obtained by culturing epidermal stem cells or keratinocytes on the surface of the dermal skin tissue; preferably epidermal stem cells;

[0107] The epidermal stem cells are 1-3 generations of epidermal stem cells derived from skin tissue; prefera...

Embodiment 3

[0139] Preparation method of vascularized bilayer engineered skin based on 3D printing, such as figure 1 shown, including the following steps:

[0140] step one( figure 1 A): Establish a polycaprolactone PCL frame structure with a height of 400 μm, and the PCL in the dotted line is as follows figure 2 , image 3 The movable PCL structure shown, can apply tension;

[0141] Step two ( figure 1 B): Set grooves on the polycaprolactone PCL frame structure, the groove depth is 90 μm, and the width is 8mm; the spacing of the PCL frame is set to 5mm and 9mm respectively according to the requirements of blood vessel branches;

[0142] Step three ( figure 1 C): The bottom of the tissue is filled with gelatin hydrogel;

[0143] Step four ( figure 1 D): printing (filling) a layer of GelMA-fibrin-adipocyte stem cell-induced differentiation in the groove to form a complex of endothelial cells and fibroblasts;

[0144] Among them, the concentration of GelMa is 25 mg / ml, and the ...

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Abstract

The invention provides a vascularized double-layer engineering skin based on 3D printing, and a preparation method thereof. The skin comprises a corium layer skin tissue and an epidermal layer skin tissue, and the corium layer skin tissue is obtained through a 3D printing mode based on traction force control; and the epidermal layer skin tissue is inoculated on the surface of the corium layer skin tissue. According to the vascularized double-layer engineering skin based on 3D printing, the corium layer skin tissue and the epidermal layer skin tissue inoculated to the surface of the corium layer skin tissue are constructed in a 3D biological printing mode, the tension force can be controlled by changing the space of a frame structure of 3D printing in the construction mode, proliferation of cells in the epidermal layer skin tissue can be effectively promoted, meanwhile, formation of vascular branches can be accurately controlled, effective support is provided for construction of the tissue engineering skin with similar normal double-layer skin tissue, and clinical application of the tissue engineering skin in the aspect of wound repair is expanded.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a vascularized double-layer engineered skin based on 3D printing and a preparation method thereof. Background technique [0002] Trauma is a common and frequently-occurring disease in clinical surgery. With the increase of traffic accidents and mechanical injuries, the number of trauma patients is increasing year by year. For the treatment of trauma, early coverage of the wound can reduce the pollution of the wound, protect the wound and prevent the aggravation of the trauma, which is of great significance for reducing the cost of treatment and the mortality rate. [0003] Currently, the tissues used clinically to cover wounds are autologous tissue, allogeneic tissue, xenogeneic tissue and tissue engineered skin. Autologous tissues are mainly skin grafts and flaps, which are derived from the patient's autologous tissues. When they are cut, secondary damage will occur; th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0698C12N2533/56C12N2533/54C12N2533/30C12N2502/1323C12N2502/28C12N2502/094C12N2502/09C12N2513/00
Inventor 张广亮
Owner SUZHOU RUIHUA HOSPITAL
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