Biocontrol bacillus for preventing and treating soil-borne diseases of fruits and vegetables and preparation of biocontrol bacillus
A technology for bacillus and soil-borne diseases, applied in the directions of biocides, fungicides, biocides, etc., can solve the problems of high cost, unfavorable promotion and application of bio-control bacteria, complicated production process, etc. Control effect, good stability effect
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[0022] The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present invention. These all belong to the protection scope of the present invention.
[0023] 1. Screening and determination of antagonistic ability of TB918 strain
[0024] Preparation of solid LB medium: 10g tryptone, 10g sodium chloride, 5g beef extract powder, 20g agar powder, distilled water to 1 liter, 100 ml Erlenmeyer flask, 121 ℃, moist heat sterilization for 30 minutes.
[0025] Preparation of solid PDA medium: 200g potatoes, 20g glucose, 20g agar powder, boiled for 30 minutes, distilled water to 1 liter, packed in 100ml Erlenmeyer flasks, sterilized by moist heat for 30 minutes at 121°C.
[0026] Use the soil dilution method to isolate and cultivate Bacillus ...
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