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Compost preparation method for performing four-stage fermentation by adopting enzyme-bacterium composite leavening agent

A compound fermentation agent and staged technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high harmful gas emissions, loss of nutrients, low humic acid content, etc., to promote reproduction , Accelerate the fermentation speed, promote the effect of rapid growth and reproduction

Active Publication Date: 2021-11-02
北京四良科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, it overcomes the shortcomings of low yield of final compost products, low content of easily oxidized organic matter and humic acid; solves the problem of difficult degradation of traditional high lignin raw materials, and the harmful effects of carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) in the composting process Technical problems with high gas emissions and loss of nutrients

Method used

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  • Compost preparation method for performing four-stage fermentation by adopting enzyme-bacterium composite leavening agent
  • Compost preparation method for performing four-stage fermentation by adopting enzyme-bacterium composite leavening agent
  • Compost preparation method for performing four-stage fermentation by adopting enzyme-bacterium composite leavening agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Prepare the enzyme-bacteria composite starter step, the enzyme-bacteria composite starter contains lignin-degrading bacteria composite agent and Bacillus licheniformis intracellular enzyme crude extract:

[0029] The lignin-degrading compound bacterial agent consists of Bacillus subtilis, Bacillus licheniformis, white rot fungus, Klebsiella, Toluene, Pseudomonas, Streptomyces rubrum, Sporiospora thermophiles, Saccharomyces cerevisiae, according to The mass ratio is 1.5:1:0.9:1:1:0.8:0.8:1.2:1, and the effective number of viable bacteria of the lignin-degrading composite bacterial agent is ≥1.00×1010 cfu·g-1;

[0030] The preparation method of the intracellular enzyme crude extract of Bacillus licheniformis is as follows: prepare the seed medium: peptone 10g / L, beef extract 3g / L, NaCl 5g / L, pH is 7.2; prepare the fermenter medium: casein peptone 15g / L, soybean peptone 5g / L, NaCl 5g / L, pH 7.3; pick a single colony of the activated Bacillus licheniformis strain, inoculate ...

Embodiment 2

[0040] The pretreatment steps of agricultural and forestry waste: cow dung and coconut shell are selected as raw materials for agricultural and forestry waste, the coconut shell is crushed to particles with a particle size of <5cm, put into a drum mixer and mixed with pig manure for 15 minutes, and the coconut shell and cow dung The mass ratio of the mixture is 3:7. During the mixing process, an enzyme-bacteria composite starter that accounts for 10‰ of the total amount of the mixture is added. After extracting liquid and mixing, adjust the moisture content of the prepared materials to 62%, the carbon-to-nitrogen ratio to 28:1, and the bulk density to 650kg / m3 to obtain fermentation raw materials; prepare the enzyme-bacteria composite starter step, and stack the fermentation raw materials. The same as Example 1, composting fermentation steps: the composting fermentation raw materials do not have tossing and no transshipment during the fermentation process, the fermentation peri...

Embodiment 3

[0046] The pretreatment steps of agricultural and forestry waste: pig manure, bamboo powder, and peanut shells are selected as raw materials for agricultural and forestry waste, and the bamboo powder and peanut shells are crushed to particles with a particle size of <5cm, and put into a drum mixer and mixed with pig manure for 15 minutes , the mass ratio of bamboo powder, peanut shells and pig manure is 1:4. During the mixing process, an enzyme-bacteria composite starter that accounts for 10‰ of the total amount of the mixture is added, and the enzyme-bacteria composite starter contains lignin-degrading bacteria. Licheniformis agent and Bacillus licheniformis intracellular enzyme crude extract, after mixing, adjust the moisture content of the raw material to 58%, the carbon-to-nitrogen ratio to 28:1, and the bulk density to 610kg / m3 to obtain the fermentation raw material; prepare the enzyme-bacteria composite starter step , the stacking steps of the fermentation raw materials ...

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Abstract

The invention relates to a compost preparation method for performing four-stage fermentation by adopting an enzyme-bacterium composite leavening agent. The compost preparation method comprises the following steps: pretreatment of agricultural and forestry wastes: crushing high-lignin waste, putting the crushed waste into a drum mixer, mixing and coordinately matching the crushed waste with the excrements of human beings, livestock and poultry, adding the enzyme-bacterium composite leavening agent in a mixing process, and adjusting the water content, the carbon-nitrogen ratio and the volume weight of the agricultural and forestry waste stock to obtain a fermentation raw material; stacking of the fermentation raw material; and fermentation of the raw material, wherein a fermentation period is 25-35 days, the fermentation is divided into four stages, namely a heating high-oxygen stage, a high-temperature low-oxygen stage, a cooling oxygen-deficient stage and a normal-temperature standing stage, and the fermentation is completed after the four stages. The technical problems that in a compost preparation process, foul smell is generated by fermentation of the excrements of human beings, livestock and poultry, high lignin waste is difficult to degrade, an emission amount of carbon and nitrogen gas is high, and compost quality is low are solved; and finally, agricultural and forestry waste is efficiently returned to the field for utilization, a soil carbon pool is increased, and carbon neutralization is achieved.

Description

technical field [0001] The present application relates to the field of agricultural compost preparation, in particular to a compost preparation method using an enzyme-bacteria composite starter for four-stage fermentation. Background technique [0002] Composting is a method of aerobic fermentation to treat organic waste. It is mainly a process of transforming organic matter into fertilizer through the fermentation of microorganisms under certain water, C / N ratio and aeration conditions under artificial control. In this composting process, organic matter is transformed from an unstable state into a stable humus substance, which is used as fertilizer and soil improvement. Studies have shown that the current basic resources of organic fertilizers in my country exceed 5 billion tons per year, including about 3 billion tons of livestock manure (fresh), about 1 billion tons of straw (air-dried), about 20 million tons of cake fertilizer, and about 90 million tons of kitchen waste....

Claims

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Application Information

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IPC IPC(8): C05F15/00C05F17/20C12N1/20C12N1/14C12N1/16C12R1/125C12R1/10C12R1/22C12R1/38C12R1/465C12R1/865C12R1/645
CPCC05F3/04C05F17/20C12N1/20C12N1/14C12N1/16C05F11/00C05F17/60Y02W30/40
Inventor 于家伊张文刘墨任忠秀
Owner 北京四良科技有限公司
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