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Application of arginine methyltransferase 3 in breast cancer diagnosis and treatment

A technology of arginine methylation and transferase, which can be applied in the fields of medical preparations containing active ingredients, measurement/testing of microorganisms, biochemical equipment and methods, etc., and can solve problems such as reports of no relationship with breast cancer

Pending Publication Date: 2021-10-22
SHANGHAI FIRST PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is no report on the relationship between PRMT3 and breast cancer

Method used

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  • Application of arginine methyltransferase 3 in breast cancer diagnosis and treatment
  • Application of arginine methyltransferase 3 in breast cancer diagnosis and treatment
  • Application of arginine methyltransferase 3 in breast cancer diagnosis and treatment

Examples

Experimental program
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Embodiment 1

[0034] 1. Experimental materials

[0035] The source of the reagents used in this example: MTT (Tetramethylazolazolium Blue) and DMSO were purchased from Sigma Company, newborn bovine serum was purchased from Hangzhou Sijiqing Biological Engineering Technology Co., Ltd., fetal bovine serum and RPMI-1640 medium were purchased from GIBCO Corporation. LipofectamineTM2000 was purchased from Invitrogen. Breast cancer cell lines were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences.

[0036] 2. Experimental method

[0037] 1. Establish a specific shRNA lentiviral library, containing a total of 88 HAT\HDAC and HMT\HDM genes

[0038] Design and synthesis of shRNA primers (Invitrogen), ligated to shRNA lentiviral vector pUCTP; vector enzyme digestion system, product recovery, ligation system; 5 μl ligation product was transformed into GeneHogs chemically competent bacteria; enzyme digestion verification; shRNA lentiviral packaging. Cell culture: 293T cells w...

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PUM

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Abstract

The invention relates to an application of arginine methyltransferase 3 (PRMT3) in breast cancer diagnosis and treatment. It is proved that the PRMT3 expression in breast cancer tumor tissues is higher than that in normal tissues, and the high PRMT3 expression and the poor prognosis of breast cancer patients show significant positive correlation; overexpression or inhibition of the PRMT3 can change breast cancer cell stemness phenotype, microsphere formation, tumor forming ability and resistance to traditional chemotherapeutic drugs, the PRMT3 promotes recurrence, metastasis and generation of treatment resistance by promoting interstitial transformation and enhancement of breast cancer cell stemness, and the regulation effect of the PRMT3 on breast cancer stemness is mediated by activating a Wnt pathway. The invention provides a new means for diagnosis, prognosis and treatment of breast cancer.

Description

technical field [0001] The invention belongs to the field of disease diagnosis and drug target, and specifically relates to the clinical diagnosis of breast cancer and the application of arginine methyltransferase 3 as a drug target. Background technique [0002] Breast cancer (Breast Cancer, BC) is the first cause of female malignant tumor morbidity and related death. The incidence of breast cancer in China shows an increasing trend year by year. Globally, China accounts for 12.2% of newly diagnosed cases and 9.6% of breast cancer deaths. Recurrence, metastasis and drug resistance are the main reasons leading to treatment failure and death of patients. Recent studies have found that, in addition to basic genetic factors, epigenetic modification (Epigenetics Modification) plays an important regulatory role in the occurrence and development of breast cancer. Epigenetic abnormalities exist in almost all human tumors, leading to changes in DNA structure and function, abnormal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/573C12Q1/6886A61K45/00A61P35/00A61P35/04
CPCG01N33/57415G01N33/573C12Q1/6886A61K45/00A61P35/00A61P35/04G01N2333/91017C12Q2600/118C12Q2600/178
Inventor 王红霞许静轩周天浩蒋梦怡鞠高达虞越
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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