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Wheat ear in-vitro culture solution for hybrid induction of haploid embryos of wheat and corn and a preparation method thereof

An in vitro culture and haploid technology, applied in the field of agricultural hybridization, can solve problems such as increasing pollution risks and increasing workload, and achieve the effects of high transparency, reduced labor costs, and improved embryo formation rate.

Active Publication Date: 2021-10-22
FOOD CROPS RES INST YUNNAN ACADEMY OF AGRI SCI
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Problems solved by technology

Since the embryo formation rate is only about 30%, that is, 70% of the caryopsis does not contain embryos, but it still needs to be identified manually under a microscope, which not only increases the workload but also increases the risk of contamination during inoculation

Method used

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  • Wheat ear in-vitro culture solution for hybrid induction of haploid embryos of wheat and corn and a preparation method thereof
  • Wheat ear in-vitro culture solution for hybrid induction of haploid embryos of wheat and corn and a preparation method thereof

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Embodiment Construction

[0034] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0035] Example, the culture solution is prepared from the following compounds and the corresponding dosage: culture solution formula potassium dihydrogen phosphate 1.25g / L, urea 1.5g / L, boric acid 100mg / L, citric acid 20mg / L, 2,4- D 50mg / L, sucrose 20g / L.

[0036] Nutrient solution preparation includes the following steps:

[0037] 1) Prepare potassium dihydrogen phosphate 40× mother liquor, that is, weigh 50g of potassium dihydrogen phosphate, put 500ml of distilled water in a 1L volumetric flask, dissolve the weighed potassium dihydrogenphosphate in the volumetric flask, set the volume to 1L, and put Store in the fresh-keeping layer of the refrigerator for later use;

[0038] 2) Prepare urea 40× mother liquor, that is, weigh 60g of urea, put 500ml of distilled water in a 1L volumetric flask, dissolve the weighed urea in the volumetric flask, set the volum...

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Abstract

The invention discloses a wheat ear in-vitro culture solution for hybrid induction of haploid embryos of wheat and corn and a preparation method thereof. The culture solution is prepared from the following compounds according to corresponding amounts: 1-1.5 g / L of mono-potassium phosphate, 1.3-1.7 g / L of urea, 80-120 mg / L of boric acid, 16-24 mg / L of citric acid, 46-54 mg / L of 2,4-D, and 15-25 g / L of sucrose. The method does not contain sulfurous acid and silver nitrate, the use amount of the used compound is halved, the nutrient solution replacement interval is prolonged from 3 days to 7 days and only needs to be replaced once, and labor investment is reduced, so that the double haploid production cost is reduced, the embryo forming rate is effectively increased, the thousand seed weight and plumpness of the caryopses are improved, and whether the caryopses have embryos or not can be detected.

Description

technical field [0001] The invention relates to the technical field of agricultural hybridization, in particular to a wheat ear in vitro culture medium for inducing haploid embryos from wheat-corn hybridization and a preparation method thereof. Background technique [0002] Double haploid (doubled haploid, DH) technology can make heterozygous breeding materials homozygous stable in one generation, accelerate the breeding process, and double the breeding efficiency. Integrating DH technology with molecular design breeding to construct a "fast and precise" high-efficiency breeding technology system has become an important development direction of high-efficiency crop breeding technology (Li at al., 2020; Kalinowska et al., 2019; Kelliher et al., 2019 ; Wang et al., 2019). In addition, haploid and double haploid also have important application value in gene function, transgenic research, and genetic population construction (Grewal et al., 2021; Liu et al., 2020). [0003] Dou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05G5/20
CPCC05B7/00C05G5/20C05C9/00C05D9/02C05F11/00
Inventor 李宏生杨忠慧李绍祥杨木军刘琨赵红
Owner FOOD CROPS RES INST YUNNAN ACADEMY OF AGRI SCI
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