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Composition containing liposome-coated thymosin as well as preparation method and application of composition

A technology of thymosin and liposomes, which is applied in the field of compositions containing liposome-encapsulated thymosin and its preparation, can solve problems such as irregular clinical observation, inconsistent preparation methods and quality control, and difficulty in determining the curative effect, and achieve enhanced The effect of basal immunity

Pending Publication Date: 2021-10-22
广州恒宁生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thymosin has been clinically used in my country for more than 20 years. In the past, due to the inconsistent preparation methods and quality control of various preparations, the clinical observation was not standardized, and the curative effect was difficult to be sure.

Method used

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  • Composition containing liposome-coated thymosin as well as preparation method and application of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation of liposome-encapsulated thymosin:

[0031] (1) Dissolve soybean lecithin, cholesterol and vitamin E in absolute alcohol, stir until completely dissolved to form liposomes, as phase I; thymosin as phase II; disodium hydrogen phosphate, sodium dihydrogen phosphate, EDTA di Sodium and HL-10 are dissolved in sterile water as phase III;

[0032] (2) Place II-phase thymosin in a high-pressure micro-jet homogenizer and sterilized water for nanometerization;

[0033] (3) Put phase I in a round bottle, turn on the equipment at a temperature of 38°C, stir at 50r / min, and vacuum -0.1mpa; stir to evaporate the alcohol until no bubbles come out of the bottle and stop;

[0034] (4) Put part of Phase III in a beaker and stir to dissolve evenly, add Phase I and Phase II, and shake until Phase I and Phase II are completely dissolved;

[0035] (5) Pour the homogeneously mixed liquid of step (4) into a beaker;

[0036] (6) Pour the mixed liquid in step (5) into a round ...

Embodiment 2

[0048] 1. Preparation of liposome-encapsulated thymosin:

[0049] (1) Dissolve soybean lecithin, cholesterol and vitamin E in absolute alcohol, stir until completely dissolved to form liposomes, as phase I; thymosin as phase II; disodium hydrogen phosphate, sodium dihydrogen phosphate, EDTA di Sodium and HL-10 are dissolved in sterile water as phase III;

[0050] (2) Place II-phase thymosin in a high-pressure micro-jet homogenizer and sterilized water for nanometerization;

[0051] (3) Put phase I in a round bottle, turn on the equipment at a temperature of 40°C, stir at 50r / min, and vacuum -0.1mpa; stir to evaporate the alcohol until no bubbles come out of the bottle and stop;

[0052] (4) Put part of Phase III in a beaker and stir to dissolve evenly, add Phase I and Phase II, and shake until Phase I and Phase II are completely dissolved;

[0053] (5) Pour the homogeneously mixed liquid of step (4) into a beaker;

[0054] (6) Pour the mixed liquid in step (5) into a round bo...

Embodiment 3

[0065] Example 3 Efficacy experiment: The composition (Example 1) containing liposome-encapsulated thymosin affects the immune system of experimental mice Results:

[0066] Kunming experimental mice were divided into two groups under normal feeding conditions, a control group and a liposome-encapsulated thymus composition group, and were fed for 30 days. On the 31st day, blood was collected, anticoagulated, then incubated with antibodies for 20 minutes, hemolyzed, centrifuged, supernatant was taken, and tested by flow cytometry. Flow cytometer models: BD, FACS, CANTO, the test results are summarized in Table 1 below:

[0067] Table 1

[0068]

[0069] The above data are based on 7 groups of lymphocyte surface markers that have practical significance for this project, and the average value of 3 samples is taken for each group. Experiments have shown that the immune function of mice is significantly improved when using the composition containing liposome-encapsulated thymos...

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Abstract

The invention discloses a composition containing liposome-coated thymosin as well as a preparation method and application of the composition. The composition is composed of a component A, a component B, a component C, a component D, a component E and a component F, wherein the component A is glyceryl stearate, PEG-100 stearate, caprylic acid / capric acid triglyceride, cetostearyl alcohol, diethylhexyl carbonate, caprylic acid / capric acid triglyceride or cyclopentasiloxane; the component B comprises xanthan gum, glycerol, propylene glycol, water, EDTA disodium and carbomer; the component C is sodium hydroxide; the component D is a traditional Chinese medicine extract; and the component F is prepared from a rhodophyta extract, a brown algae extract, sorbitol, sodium hyaluronate, a rice extract, methylisothiazolinone, phenoxyethanol and essence. The composition containing the liposome-coated thymosin prepared by the invention can enhance the immunity of an organism, also has the effects of diminishing inflammation, removing tinea and resisting oxidation, and can be applied to cosmetics and medicines.

Description

technical field [0001] The invention relates to a composition, in particular to a composition containing liposome-encapsulated thymosin and its preparation method and application. Background technique [0002] Thymosin (also known as thymosin, Thymosin) is a group of physiologically active polypeptides secreted by thymus tissue. Thymosin commonly used clinically is a small molecular polypeptide with non-specific immune effect discovered and purified from calf thymus. Thymosin can continuously induce T cell differentiation and development at various stages, maintain the immune balance of the body and enhance the response of T cells to antigens, thereby improving the body's ability to resist diseases. Thymus contains a variety of hormones, which belong to three categories: Co-induce the maturation and differentiation of T cells. The main active ingredient of thymosin is thymosin α1 (Tα1), which is composed of 28 amino acids, and chemically synthesized commercial products are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/22A61K9/127A61K47/10A61K47/14A61K47/34A61K47/36A61P17/00A61P29/00A61P31/10A61P37/04A61P39/06A61K8/9794A61K8/9789A61K8/9717A61K8/9711A61K8/34A61K8/14A61K8/73A61K8/49A61Q19/08A61K31/045A61K31/125A61K36/896A61K36/899A61K36/90A61K36/904
CPCA61K38/2292A61K36/75A61K36/489A61K36/90A61K36/896A61K36/756A61K36/238A61K36/23A61K31/125A61K31/045A61K36/185A61K36/904A61K36/12A61K36/748A61K36/899A61K36/04A61K36/03A61K9/127A61K47/36A61K47/34A61K47/14A61K47/10A61P37/04A61P29/00A61P17/00A61P31/10A61P39/06A61K8/9794A61K8/9789A61K8/9717A61K8/9711A61K8/34A61K8/345A61K8/14A61K8/735A61K8/49A61Q19/08A61K2800/5922A61K2300/00
Inventor 林彤周丹丹贺爱国
Owner 广州恒宁生物科技有限公司
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