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Application of reagent for down-regulating RBMS1 expression in preparation of medicine for treating lung cancer

A technology of reagents and drugs, applied in the field of diagnostic kits, can solve problems such as the lack of lung cancer targets

Active Publication Date: 2021-10-15
DALIAN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Lung cancer-related targets are still lacking

Method used

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  • Application of reagent for down-regulating RBMS1 expression in preparation of medicine for treating lung cancer
  • Application of reagent for down-regulating RBMS1 expression in preparation of medicine for treating lung cancer
  • Application of reagent for down-regulating RBMS1 expression in preparation of medicine for treating lung cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0175] Lung cancer patients with high RBMS1 expression have shorter overall survival

[0176] figure 1 The expression of RBMS1 in the tissues of lung cancer patients. The expression of RBMS1 in normal and tumor tissues of 7 patients with lung cancer was analyzed by western blotting. figure 1 A in the middle is the western blot detection of RBMS1 expression in normal tissue (N) and cancer tissue (T) of lung cancer patients, the results show that RBMS1 is highly expressed in lung cancer tissue; 60 pairs of human lung cancer tissue (NSCLC) and adjacent normal tissues (Normal) RBMS1 immunohistochemical staining was performed (n=60 in lung cancer tissues, n=60 in adjacent normal tissues), and the expression level of RBMS1 was divided into three levels according to immunohistochemical staining Positive (strongpositive), super-strong positive (extra-strongpositive); figure 1 The B in the middle is a representative graph of the expression of RBMS1 in human lung cancer tissues (NSCL...

Embodiment 2

[0178] Knockdown of RBMS1 inhibits lung cancer cell proliferation in vitro and in vivo

[0179] RBMS1 protein levels were detected in two lung fibroblasts (MRC5 and HFL1) and six lung cancer cells (A549, H460, H2170, H1299, H358, H446). figure 2 It is the result of detecting the protein expression of RBMS1 in different cell lines by western blot; in the figure, MRC5 and HFL1 are normal human lung fibroblasts, A549, H460, H2170, H1299, H358, H446 are human lung cancer cell lines, and GAPDH is used as Internal reference. The results showed that compared with human normal lung fibroblasts, the protein expression of RBMS1 was highly expressed in lung cancer cell lines.

[0180] A stable cell line with low expression of RBMS1 was constructed in H1299 and A549 lung cancer cells. Two shRNAs were designed, and H1299 and A549 were successfully constructed by infecting H1299 and A549 with pLKO.1-RBMS1-sh1 and pLKO.1-RBMS1-sh2 lentivirus systems. Knockdown cell line and control cell l...

Embodiment 3

[0185] Knockdown of RBMS1 inhibits lung cancer cell migration and invasion

[0186] Using pLKO.1-RBMS1-sh1 to knock down RBMS1 in stably transfected cell lines, the effect of RBMS1 on the migration and invasion of lung cancer cells was investigated by transwell (matrigel-free and matrigel-coated) experiments. Image 6 is the result of detecting the effect of RBMS1 on the migration and invasion ability of H1299 lung cancer cells in vitro by transwell experiment; among them, picture A is the effect of knockdown of RBMS1 on the migration ability of H1299 lung cancer cells detected by Transwell (without Matrigel) experiment, in 24-well plate Put in a Transwell chamber, slowly add 600 μL of medium containing 20% ​​serum to the lower chamber, take the pLKO.1-RBMS1-sh1 knockdown H1299 cells in logarithmic growth phase and the pLKO.1 empty H1299 cells and digest them into cells The suspension was resuspended by adding serum-free medium, and counted with a cell counter. A suspension c...

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Abstract

The invention relates to application of a reagent for down-regulating RBMS1 expression in preparation of a medicine for treating lung cancer, and belongs to the technical field of diagnostic kits. The invention provides application of a reagent for down-regulating RBMS1 expression in preparation of a medicine for treating lung cancer. The application disclosed by the invention is helpful for realizing the preparation of the medicine for treating the lung cancer.

Description

technical field [0001] The invention relates to the technical field of diagnostic kits, in particular to the application of a reagent for down-regulating the expression of RBMS1 in the preparation of a medicament for treating lung cancer. Background technique [0002] Lung cancer is the malignant tumor with the highest morbidity and mortality worldwide. In-depth understanding of the pathogenesis of lung cancer, the discovery of important links and key factors in the process of disease progression, and the search for new therapeutic targets are important issues to be solved to achieve effective prevention and treatment and improve prognosis. [0003] RNA-binding proteins are involved in the regulation of all steps at the post-transcriptional level and are key regulators of gene regulation at the post-transcriptional level, affecting basic pre-mRNA splicing, polyadenylation, stability, localization, translation into protein, and degradation. Cellular processes that determine ...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61P11/00A61P35/00A61P35/04C12N15/113C12N15/85C12Q1/6886
CPCA61K45/00A61P11/00A61P35/00A61P35/04C12Q1/6886C12N15/85C12N15/1135C12Q2600/158
Inventor 汪洋张文静祁旸凡张金瑞赵瑾瑶孙玉赵庆芝白璐智莉莉
Owner DALIAN MEDICAL UNIVERSITY
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