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Preparation method and application of multifunctional bionic nano-drug

A biomimetic nano- and multi-functional technology, which can be used in drug combinations, pharmaceutical formulations, medical preparations with inactive ingredients, etc., can solve problems such as limited biological benefits, and achieve improved antitumor effects, good biocompatibility, high The effect of applying value

Pending Publication Date: 2021-10-15
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although more and more biomembranes are developed and applied, the biological benefits inherited from a single cell membrane are still limited, and it is difficult for a single membrane component to solve multiple biological problems simultaneously.

Method used

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  • Preparation method and application of multifunctional bionic nano-drug
  • Preparation method and application of multifunctional bionic nano-drug
  • Preparation method and application of multifunctional bionic nano-drug

Examples

Experimental program
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Effect test

Embodiment 1

[0029] In a specific implementation, the preparation method of the multifunctional biomimetic nano-medicine specifically includes the following steps:

[0030] 1) Preparation of PLGA-GA nanoparticles

[0031] Prepare PLGA-GA nanoparticles by nanoprecipitation method, weigh 3mg GA and 30mg PLGA (molecular weight is 13kDa), add 3mL acetone to dissolve the organic phase, then slowly add 3mL organic phase to 9mL aqueous solution containing 1%PVA (the quality of PVA is 0.09g), stirred and volatilized at room temperature (25°C) for 10 hours to remove acetone, centrifuged at 12000rpm for 30 minutes and washed twice with ultrapure water to obtain PLGA-GA nanoparticles;

[0032] 2) Extraction of thylakoid membrane

[0033] Wash 200g of spinach and pre-cool overnight at 4°C, then mix the spinach leaves with 600mL homogeneous buffer (5mM MgCl 2 , 50mM NaCl, 0.3M sucrose and 50mM HEPES buffer, pH=7.6) were homogenized together, the homogenate was filtered through 10 layers of gauze, the...

Embodiment 2

[0039] In specific implementation, the preparation method of the multifunctional biomimetic nanomedicine specifically includes the following steps:

[0040] 1) Preparation of PLGA-GA nanoparticles

[0041] PLGA-GA nanoparticles were prepared by nanoprecipitation method. Weigh 5mg GA and 50mg PLGA (molecular weight 13kDa) respectively, add 5mL acetone to dissolve the organic phase, and then slowly add 5mL organic phase to 15mL aqueous solution containing 1% PVA (The mass of PVA is 0.15g), 25°C stirring and volatilizing for 10h, volatilizing and removing acetone, centrifuging at 12000rpm for 30 minutes and washing twice with ultrapure water to obtain PLGA-GA nanoparticles;

[0042] 2) Extraction of thylakoid membranes

[0043] Wash 300 g of lettuce, pre-cooled overnight at 4°C, and then use a wall breaker to mix the lettuce with 900 mL of homogeneous buffer (5 mM MgCl). 2 , 50mM NaCl, 0.3M sucrose and 50mM HEPES buffer, pH=7.6) were homogenized together, the homogenate was fil...

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Abstract

The invention relates to a preparation method and application of a multifunctional bionic nano-drug, which can effectively enhance the photodynamic therapy of tumors, and adopts the technical scheme that the multifunctional bionic nano-drug is characterized in that a chemotherapeutic drug gambogic acid is entrapped on a polylactic acid-glycolic acid copolymer to obtain a drug-loaded nano-core, the capsule-like membrane-cancer cell membrane composite membrane is coated on the outer side of the nano inner core through ultrasonic extrusion to form the multifunctional bionic nano drug.The multifunctional bionic nano-drug TCPG prepared by the preparation method disclosed by the invention has good biocompatibility; The anti-tumor effect of the nano-drug is effectively improved through a strategy of combining specific homologous targeting and enhanced photodynamic therapy, and the nano-drug is an innovation in nano-drugs.

Description

technical field [0001] The invention relates to the field of nano-medicine, in particular to a preparation method and application of a multifunctional biomimetic nano-medicine. Background technique [0002] At present, malignant tumor is a common disease with high morbidity and mortality, which seriously threatens human health. Traditional tumor clinical treatment methods mainly include surgical resection, radiotherapy and chemotherapy, etc., but these treatment methods have their own limitations. Among many clinical treatment strategies, photodynamic therapy has become a potential clinical alternative therapy due to its high selectivity, non-invasiveness and repeatable treatment characteristics. However, the application of photodynamic therapy is also limited by the poor tumor targeting of photosensitizers, hypoxia in tumor tissues, and excessive intracellular glutathione levels, which affect its lethality to tumor cells. Therefore, there is an urgent need to overcome the...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K31/352A61K41/00A61K47/34A61P35/00
CPCA61K9/5184A61K31/352A61K41/0076A61K47/34A61P35/00A61K2300/00
Inventor 祁迎秋王卉孟小草刘晨杜安妮孙小凡闵欢聂广军
Owner ZHENGZHOU UNIV
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