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Quantum dot modified protein vaccine as well as preparation method and application thereof

A protein vaccine and quantum dot technology, applied in the field of quantum dot modified protein vaccine and its preparation, can solve the problems of changing and enhancing the immunogenicity of cancer cells, and achieve enhanced immunogenicity, better immune activation characteristics, and enhanced specificity The effect on the probability of recognition

Pending Publication Date: 2021-10-01
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these approaches cannot inherently alter or enhance the immunogenicity of cancer cells to a large extent

Method used

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  • Quantum dot modified protein vaccine as well as preparation method and application thereof
  • Quantum dot modified protein vaccine as well as preparation method and application thereof
  • Quantum dot modified protein vaccine as well as preparation method and application thereof

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preparation example Construction

[0050] In addition, the present application also provides a method for preparing the above-mentioned quantum dot modified protein vaccine, which includes the following steps:

[0051] S1. Preparation of quantum dots.

[0052] The preparation method of quantum dot particles comprises:

[0053] Mix and dissolve the precursor carbon source and the functionalized raw material in a solvent, heat the reaction at 110-220°C for 2-10 hours, wash and separate the solid and liquid, and dry the separated solid product;

[0054] Preferably, the precursor carbon source includes at least one of citric acid, glucose and polyethylene glycol; the functionalized raw material includes at least one of urea, biuret and triuret. In this application, the quantum dot particles are synthesized by mixing the precursor carbon source and the functionalized raw material through heat treatment. Since the molecular or ion state of the precursor carbon source is very small, it can make the surface of the quan...

Embodiment 1

[0064] The present embodiment provides a kind of quantum dot modified bovine serum albumin (CQDs-BSA) vaccine, and its preparation method comprises:

[0065] Dissolve 2g citric acid and 8g urea in 30mL DMSO solvent according to the mass ratio to obtain a transparent solution. Place it in a 50mL polytetrafluoroethylene autoclave and react at 160°C for 4 hours. After the reaction, add a large amount of ethanol to wash the solution to obtain a black solid , the solid was washed with water, centrifuged (8000rpm, 5min), and dried to obtain a dark blue powder. The obtained quantum dots were determined to have a diameter of 3-10 nm. The mass ratios of C, N, O and S elements in the quantum dots are 50.1%, 29.3%, 19.1% and 1.5%, respectively.

[0066] Dissolve the obtained quantum dots in deionized water, prepare a solution with a concentration of 0.1mg / mL, mix the quantum dots and bovine serum albumin at a mass ratio of 1:10, heat up to 56°C by oven heating, react for 10min, and simu...

Embodiment 2

[0068] The present embodiment provides a kind of quantum dot modified breast cancer cell whole target protein vaccine (CQDs-W4T1), and its preparation method comprises:

[0069] Dissolve citric acid and biuret in water (or ultrapure water, PBS buffer, etc.) at a mass ratio of 1:3 to obtain a transparent solution, place it in a 50ml polytetrafluoroethylene autoclave, and react at 200°C for 3h. A large amount of ethanol was added to the reacted solution to obtain a black solid, which was washed with water, centrifuged (8000 rpm, 5 min), and dried to obtain a dark blue powder.

[0070] Dissolve the above-mentioned quantum dots in deionized water, the concentration is 0.1mg / mL, and add 1×10 7 4T1 breast cancer tumor digesting protein (W4T1). Heating methods such as water bath heating were used to raise the temperature to 56° C., and reacted for 10 minutes while stirring at a speed of 100 rpm. The whole target protein vaccine of breast cancer cells modified by quantum dots (CQDs-...

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Abstract

The invention discloses a quantum dot modified protein vaccine as well as a preparation method and application thereof, which relates to the field of nano materials and biomedicine. Raw materials of the quantum dot modified protein vaccine comprise quantum dot particles and antigen protein, the surfaces of the quantum dot particles comprise biocompatible functional groups, and the antigen protein and the functional groups act to enable the protein to be wound on the surfaces of the quantum dot particles under the action of non-covalent bonds. The antigen protein is modified through the quantum dots, the spatial conformation of the antigen protein can be changed, the immunogenicity of normal protein is remarkably enhanced due to conformation modification, and meanwhile the function of immunosuppressive protein on cancer cells is deregulated. And the specific recognition probability can be enhanced. According to the quantum dot modified protein vaccine, the immunogenicity of protein can be greatly improved, so that the quantum dot modified protein vaccine has better immune activation characteristics, the preparation method is simple, and the operation conditions are mild. The obtained quantum dot modified protein vaccine can be applied to personalized immunotherapy of cancers.

Description

technical field [0001] The invention relates to the fields of nanomaterials and biomedicine, in particular to a quantum dot modified protein vaccine and its preparation method and application. Background technique [0002] Cancer is one of the leading causes of death worldwide. Cancer cells exhibit a highly diverse mutational composition with limited overlap between different patients. Personalized cancer vaccines are vigorously developed vaccine strategies in cancer immunotherapy aimed at modulating the innate and adaptive immune systems to broadly activate anticancer immunity with individual cancer neoantigens. Currently, the process of identifying and recombinantly generating specific neoantigens is expensive and time-consuming, during which changes in neoantigens will lead to ineffective anticancer responses. In addition, anticancer vaccines targeting multiple neoantigens are thought to be more effective than anti-inflammatory vaccines targeting only a single cancer ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K39/385A61P35/00C07K1/107C01B32/15
CPCA61K39/0011A61K39/385A61P35/00C07K1/107C01B32/15A61K2039/60
Inventor 曲松楠汤子康梁桃邓初夏雷海鹏
Owner UNIVERSITY OF MACAU
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