Primer group for LAMP (loop-mediated isothermal amplification) detection of novel 2019 coronavirus, dual constant-temperature chromogenic kit and detection method

Abstract

The invention provides a primer group for LAMP (loop-mediated isothermal amplification) detection of novel 2019 coronavirus, a dual constant-temperature chromogenic kit and a detection method. The dual constant-temperature chromogenic kit comprises an outer primer pair 1, an outer primer pair 2, an inner primer pair 1, an inner primer pair 2, a loop primer pair 1 and a loop primer pair 2. The 2019 novel coronavirus double constant-temperature chromogenic screening detection kit provided by the invention has the advantages of mature and stable technology, low test cost and more suitability for primary popularization, is more suitable for rapid screening detection of primary laboratories such as community laboratories, enterprise laboratories and county health institutes, has good market competitiveness, and has good industrialization prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a primer set for 2019 novel coronavirus LAMP detection, a dual constant temperature chromogenic kit and a detection method. Background technique [0002] There are some defects in the existing 2019 coronavirus detection reagents, such as PCR technology is simple and fast, high sensitivity, but heavy workload, cumbersome operation, low throughput; gene chip high throughput, but also easy to cause false positives, expensive; real-time fluorescence Quantitative PCR technology has high sensitivity, good reliability, and is a fully closed reaction. However, this technology requires expensive fluorescent quantitative PCR instruments, which cannot be popularized in grass-roots inspection departments and epidemic prevention institutions, and fluorescently labeled probes are required to ensure high specificity. higher cost. In short, these nucleic acid detection technologies have...

AI Technical Summary

Problems solved by technology

[0002] There are some defects in the existing 2019 coronavirus detection reagents, such as PCR technology is simple and fast, high sensitivity, but the workload is heavy, the operation is cumbersome, and the throughput is low; the throughput of the gene chip is high, but it is also easy to cause false positives and is expensive; real-time fluorescence Quantitative PCR technology has high sensitivity and good reliability, and it is a fully closed reaction. However, this technology requires expensive fluorescent quantitative PCR instruments, which cannot be popularized in grassroots detection departments and epidemic prevention institutions, and fluorescently labeled probes are required to ensure high specificity. higher cost

In short, these nucleic acid detection technologies have a series of shortcomings such as relying on expensive instruments, high detection costs, and laboratory limitations; moreover, these molecular biology detections have high requirements for quality control, operating environment and professional ability of personnel, and cannot Achieving popularization and application at the grassroots level cannot meet the needs of rapid virus detection during the epidemic

The operation of constant temperature chromogenic amplification is simple, convenient and does not require highly skilled staff to operate. However, due to the difficulty in designing primers, false negatives or false positives are prone to occur, resulting in a shortage of reagents for constant temperature chromogenic amplification.

[0003] At present, the quality of nucleic acid detection kits is uneven, and the stability and reliability of nucleic acid detection are still doubtful; the detection rate of nucleic acid is low, and many medical records need to be tested 2-3 times; Virus; the number of people waiting for nucleic acid testing greatly exceeds the testing capacity, and the test results obtained in a hurry are not reliable

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