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Tea tree orphan gene csog3 and its application in improving cold tolerance of tea plants

A cold tolerance, tea tree technology, applied in the field of genetic engineering, to achieve the effect of enriching cognition

Active Publication Date: 2022-03-18
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the regulation of tea tree orphan genes in the regulation of tea tree response to adversity stress, especially low temperature stress

Method used

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  • Tea tree orphan gene csog3 and its application in improving cold tolerance of tea plants
  • Tea tree orphan gene csog3 and its application in improving cold tolerance of tea plants
  • Tea tree orphan gene csog3 and its application in improving cold tolerance of tea plants

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Embodiment 1

[0019] 1. Cloning and sequence structure analysis of tea tree orphan gene CsOG3

[0020] Sequence cloning was carried out using the young leaves of tea tree national-level fine variety Shuchazao (planted in the National High-Tech Agricultural Park of Anhui Agricultural University, 31°56′N, 117°12′E) as materials. The total RNA was extracted using Tiangen’s Spin Column Type Plant Total RNA Rapid Extraction Kit, and was carried out according to the operation steps in the kit manual. The total RNA content and quality were detected using a nucleic acid protein quantifier and gel electrophoresis.

[0021] Synthesize cDNA by reverse transcription: take 2 μg of total RNA as a template, and perform reverse transcription reaction according to TaKaRa’s PrimeScript II 1stStrand cDNA Synthesis Kit kit, as follows: add 0.6 μg of Oligo dTPrimer (50 μM) and 0.4 μl of Random 6mers (50 μM) respectively , dNTP Mixture (10mM each) 1.0μl, and use RNase Free dH 2 O was adjusted to 10 μL, denatur...

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Abstract

The invention belongs to the technical field of genetic engineering and discloses a tea tree orphan gene CsOG3 and its application in improving the cold resistance of tea trees. The nucleotide sequence of the tea tree orphan gene CsOG3 is shown in SEQ ID NO.1 in the sequence listing; the amino acid sequence of the protein encoded by the tea tree orphan gene CsOG3 is shown in SEQ ID NO.2 in the sequence listing. In the present invention, the molecular mechanism of the tea tree orphan gene CsOG3 involved in responding to low temperature stress is cloned and verified for the first time, and a recombinant plasmid, transgenic engineering bacteria and transgenic plants containing the CsOG3 gene are also provided. The invention enriches the theory of molecular regulation mechanism of tea trees coping with low temperature stress, provides important target genes and reference basis for tea tree resistance breeding, and has great application value.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to tea tree orphan gene CsOG3 and its application in improving the cold resistance of tea trees. Background technique [0002] Tea tree (Camellia sinensis (L.) O. Kuntze) is an important economic crop in my country. As a perennial evergreen woody plant, tea tree has been subjected to different adversity stresses during its growth, such as low temperature, drought, high temperature, insect pests and other biotic and abiotic stresses. With the increase of demand for tea, the area of ​​global tea gardens needs to be continuously expanded to meet the supply. These biotic and abiotic stresses have greatly limited the growth of tea trees and the expansion of planting area. In this regard, researchers have also carried out in-depth research on the stress resistance of tea trees to understand the response mechanism of tea trees under adversity stress, in order to cultivate new ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/00A01H6/82
CPCC07K14/415C12N15/8273C12N15/8205
Inventor 夏恩华葛若蘅童伟吴琼杨天元
Owner ANHUI AGRICULTURAL UNIVERSITY
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