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Composite high-temperature microbial inoculum and application thereof in plastic degradation

A high-temperature bacteria and plastic technology, applied in the field of environmental purification, can solve the problems of low degradation rate and inability to remove microplastics, achieve efficient removal and broaden the application range

Active Publication Date: 2021-09-17
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since MPs are mostly high-molecular polymers with strong hydrophobicity, most microorganisms have a low degradation rate in the natural environment, resulting in the inability to remove microplastics in the actual composting process.

Method used

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  • Composite high-temperature microbial inoculum and application thereof in plastic degradation
  • Composite high-temperature microbial inoculum and application thereof in plastic degradation
  • Composite high-temperature microbial inoculum and application thereof in plastic degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The preparation of embodiment 1 composite high-temperature bacterial agent

[0058] The preparation method is as follows:

[0059] (1) Preparation of seed solution:

[0060] According to the inoculum size of 2%, the concentration is 10 8 Cfu / mL of Bacillus composti SgZ-9 bacteria liquid is inoculated into the container with LB medium, and fermented at 50°C for 48 hours. During the fermentation process, air needs to be introduced, and the ventilation rate is 20-100m 3 / h, and continuously stirred at a speed of 180-220 rpm, and the Bacillus composti SgZ-9 seed liquid was obtained after the fermentation was completed.

[0061] (2) Preparation of anaerobic fermentation medium:

[0062] Mix 0.6g / L sodium dihydrogen phosphate, 0.25g / L ammonium chloride, 0.1g / L potassium chloride, 0.2g / L yeast extract (yeast powder, purchased from Aladdin), 0.5g / L glucose, Add 3% (mass ratio) ferrihydrite, adjust the pH to 7.0, and stir evenly. Pass nitrogen / carbon dioxide mixed gas into ...

Embodiment 2

[0065] The preparation of embodiment 2 composite high-temperature bacterial agents

[0066] The preparation method is as follows:

[0067] (1) Preparation of seed solution:

[0068] According to the inoculum size of 2%, the concentration is 10 8 Cfu / mL of Bacillus thermophilus SgZ-10 bacteria liquid is inoculated into the container with LB medium, and fermented at 50°C for 48 hours. During the fermentation process, air needs to be introduced, and the ventilation rate is 20-100m 3 / h, and continuously stirred at a speed of 180-220 rpm, and the Bacillus thermophilusSgZ-10 seed liquid was obtained after the fermentation was completed.

[0069] (2) Preparation of anaerobic fermentation medium:

[0070] Mix 0.6g / L sodium dihydrogen phosphate, 0.25g / L ammonium chloride, 0.1g / L potassium chloride, 0.2g / L yeast extract (yeast powder, purchased from Aladdin), 0.5g / L glucose, Add 5% (mass ratio) hematite, adjust the pH to 7.5, and stir evenly. Pass nitrogen / carbon dioxide mixed gas...

Embodiment 3

[0073] The preparation of embodiment 3 composite high-temperature bacterial agents

[0074] The preparation method is as follows:

[0075] (1) Preparation of seed solution:

[0076] According to the inoculum amount of each 2% of each plant, the concentration was 10 8 cfu / mL of Bacillus composti SgZ-9 bacteria solution and a concentration of 10 8 Cfu / mL of Bacillus thermophilus SgZ-10 bacteria solution was inoculated into the container with LB medium respectively, and fermented at 50°C for 24 hours. During the fermentation process, air was required, and the ventilation rate was 20-100m 3 / h, and continuously stirred at a speed of 180-220 rpm, after the fermentation is completed, Bacillus composti SgZ-9 and Bacillusthermophilus SgZ-10 seed liquids are obtained.

[0077] (2) Preparation of anaerobic fermentation medium:

[0078] Mix 0.6g / L sodium dihydrogen phosphate, 0.25g / L ammonium chloride, 0.1g / L potassium chloride, 0.2g / L yeast extract (yeast powder, purchased from Alad...

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Abstract

The invention discloses a composite high-temperature microbial inoculum and application thereof in plastic degradation. The composite high-temperature microbial inoculum contains thermophilic iron reducing bacteria and iron minerals. According to the composite high-temperature microbial inoculum, microorganisms can grow and breed by directly utilizing nutrient substances in organic solid wastes under the aerobic condition, Fe (II) of the iron minerals in the composite high-temperature microbial inoculum is oxidized into Fe (III), and hydroxyl radicals are generated, so that the surface hydrophobicity of a plastic material is destroyed, and oxidative degradation of micro-plastics in compost is promoted. Under an anoxic condition, Fe (III) is reduced into Fe (II) by the thermophilic iron reducing bacteria, therefore, by changing the dynamic change of the aerobic-anoxic region in the composting process, the continuous process of hydroxyl radicals by iron redox cycle in the compost can be promoted, and the efficient removal of the micro-plastics in the compost can be realized.

Description

technical field [0001] The invention belongs to the field of environmental purification, and in particular relates to a composite high-temperature bacterial agent and its application in plastic degradation. Background technique [0002] Microplastics (MPs), which refer to plastic particles with a size smaller than 5mm, are new types of pollutants that are widely distributed today. MPs have small particle size, large quantity, and mostly contain toxic additives, making them easy to be eaten or absorbed by organisms in the environment to cause physiological damage, and they are also easy to absorb other pollutants, form pollutant complexes, and migrate in the environment. And endanger human health through the food chain. MPs mainly exist in organic solid wastes such as domestic garbage, feces and municipal sludge. According to the survey, the concentration of microplastics in urban sludge can reach 1.6-5.6×10 4 Each kilogram of sludge (dry weight), about 44,000 to 430,000 t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C05F17/50C05F17/70C05G3/00C12R1/01C12R1/07
CPCC12N1/20C05F3/00C05F17/20C05F17/50C05F17/70C05G3/00C05F11/00C05F11/08Y02W30/40
Inventor 陈志邢睿智周顺桂
Owner FUJIAN AGRI & FORESTRY UNIV
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