Duck reovirus and duck circovirus bivalent inactivated vaccine and preparation method thereof

A dual inactivated vaccine, duck circovirus technology, applied in biochemical equipment and methods, viruses, vaccines, etc.

Pending Publication Date: 2021-09-14
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no effective measures to prevent and control these two diseases, so it is particularly important to develop a safe and effective dual inactivated vaccine as soon as possible

Method used

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  • Duck reovirus and duck circovirus bivalent inactivated vaccine and preparation method thereof
  • Duck reovirus and duck circovirus bivalent inactivated vaccine and preparation method thereof
  • Duck reovirus and duck circovirus bivalent inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation of embodiment 1 duck regurgitation arc, duck circular double inactivated vaccine

[0030] 1 poison seed

[0031] Duck Reovirus S strain, its microorganism preservation number is: CGMCC No.20000; preservation unit: General Microbiology Center of China Committee for the Collection of Microorganisms; preservation date: July 6, 2020; classification name: new type of duck reovirus Arc virus; preservation address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0032] 2 Preparation method

[0033] 2.1 Preparation of duck circobaculovirus antigen

[0034] 2.1.1 Design and synthesis of duck circovirus CAP gene

[0035] After the duck circovirus cap gene was codon-optimized, EcoRI and Hind III restriction sites were added at both ends of the sequence, and the biological company was entrusted to clone it into the pFastBacⅠ vector to form a recombinant expression plasmid pFB-DUCV-cap.

[0036] 2.1.2 Construction and preparation of recombinan...

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PUM

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Abstract

The invention discloses a duck reovirus and duck circovirus bivalent inactivated vaccine and a preparation method thereof. The bivalent inactivated vaccine comprises an effective amount of inactivated antigen and an immunologic adjuvant in immunization, wherein the inactivated antigen comprises duck reovirus antigen and duck circovirus antigen proteins. The invention further discloses a preparation method of the bivalent inactivated vaccine. The bivalent inactivated vaccine provided by the invention is good in safety, does not generate mutual interference or influence of antigen components, can achieve the protection effect of secondary immunization of each single vaccine through one-time immunization, can prevent duck reovirus and duck circovirus at the same time, and can avoid adverse reaction caused by multiple times of immunization, and the preparation method has the advantages of simplicity, convenience, multiple effects, low cost, high vaccine titer content and the like.

Description

technical field [0001] The invention relates to a dual inactivated vaccine for preventing and treating duck virus disease, in particular to a dual inactivated vaccine for duck reovirus and duck circovirus. The invention further relates to a preparation method of the dual inactivated vaccine, which belongs to duck Reovirus, duck circovirus dual vaccine and its preparation field. Background technique [0002] New duck reovirus disease is a disease characterized by spleen enlargement, hemorrhage, and necrosis. Since 2017, it has been found in duck farms in Shandong, Henan, and Jiangsu. The susceptible days are mostly in the range of 5d-25d, the incidence rate is generally between 10%-70%, and the mortality rate is generally between 50%-60%. The younger the age, the higher the morbidity and mortality. Both morbidity and mortality increased significantly in cold weather environments. [0003] Duck circovirus mainly invades the immune system of the host, resulting in low immune...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/15A61K39/12A61P31/14A61P31/20
CPCA61K39/12A61P31/14A61P31/20C12N2720/12234C12N2750/10034A61K2039/552A61K2039/5252A61K2039/70
Inventor 梁宛楠宋扬宋莹李应鹤徐刚胡秀艳刘洋李春雪曹宝珠吴强
Owner 哈药集团生物疫苗有限公司
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