Miniaturized Polarized Point Diffraction Digital Holographic Microscopy Device Based on LED Illumination

Abstract

The invention discloses a miniaturized polarization point diffraction digital holographic microscope device based on LED illumination, comprising a partially coherent light generation and regulation module, a light size control module, a telescope system, a material parameter light separation module and The image acquisition module, wherein the partially coherent light generation and control module is used to generate partially coherent light and control its polarization characteristics; the light size control module is used to scale the size of the polarized light and generate uniform illumination light waves; the telescope system is used to The sample is enlarged and imaged to form a light field distribution with sample information; the object parameter light separation module is used to diffract the light with sample information to generate object light and reference light; the image acquisition module is used to collect the object light and reference light generated by hologram. Since the object light and the reference light pass through the same optical element, the present invention has very good immunity to environmental disturbance; at the same time, the image quality is greatly improved due to the use of low-coherence LEDs for illumination.

Description

Miniaturized Polarization Spot Diffraction Digital Holographic Microscope Based on LED Illumination technical field The invention belongs to the technical field of optical microscopy imaging, be specifically related to a kind of miniaturized polarization point based on LED illumination Diffraction digital holographic microscopy for label-free, high-contrast, and high-resolution in situ inspection of transparent samples. Background technique In current biological research and medical diagnosis, single cell or biological tissue reflects the life state of an organism. main object. Electron microscopes, atomic force microscopes, etc. have nanometer or even sub-nanometer spatial resolution, which can detect The fine structure of various subcellular organelles provides reliable detection methods and important scientific methods for the diagnosis and treatment of many diseases. in accordance with. Since these techniques have special requirements for sample preparation and...

AI Technical Summary

Problems solved by technology

It should be noted that the reference light in this technique is generated by filtering the object light wave, so the light intensity of the reference light is directly related to the scattering degree of the measured sample, and there is no guarantee that the interference fringes will have a good appearance under any sample. contrast, the accuracy of the reconstruction cannot be guaranteed

In addition, the traditional digital holographic microscopy technology uses highly coherent laser light for illumination, and can obtain a high-contrast hologram. However, the high coherence causes the image to have serious speckle noise, which reduces the sensitivity and accuracy of the measurement.

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