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Nucleic acid micro-damage detection method based on field effect transistor and biosensor

A field effect transistor and biosensor technology, which is applied in the field of nucleic acid micro-damage detection methods and biosensors based on field effect transistors

Active Publication Date: 2021-09-10
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require high doses of damaging agents and highly enriched nucleic acids to ensure signal amplification, which makes them unsuitable for in vivo detection at pM concentrations (Gietema, J.A., et al.Lancet 2000, 355, 1075-1076 .) The low incidence of DNA damage caused by ultra-low doses of drugs, so it is urgent to develop a highly sensitive method that can detect DNA damage caused by pM level gene-damaging drugs (such as cisplatin)

Method used

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  • Nucleic acid micro-damage detection method based on field effect transistor and biosensor
  • Nucleic acid micro-damage detection method based on field effect transistor and biosensor
  • Nucleic acid micro-damage detection method based on field effect transistor and biosensor

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Such as figure 1 The field-effect transistor-based biosensor shown has a bottom-gate top-contact structure. The biosensor comprises: a gate 1, an insulating layer 2, a semiconductor layer 3, a source electrode 4, a drain electrode 5, an electrode protection layer 6 and a micro-reservoir 9 from bottom to top. The source electrode 4 and the drain electrode 5 are arranged on the surface of the semiconductor layer 3, and gold nanoparticles 7 are fixed on the surface of the semiconductor layer 3, and the nucleic acid molecule connection site layer is formed by the gold nanoparticles. Gold nanoparticles 7 are linked to nucleic acid molecules 8 .

[0046] The micro reservoir 9 has a side wall and a top surface and a bottom surface surrounded by the side wall, the bottom surface is closed by the nucleic acid molecule connection site layer, the top surface is not closed, and the top surface has a circular opening with a radius of 2mm; wherein the side wall The material is PDMS...

Embodiment 2

[0058] According to the detection method of Example 1, only the solution to be tested in the reservoir is replaced with a solution containing carboplatin or oxaliplatin. Test the double-stranded DNA damage caused by carboplatin and oxaliplatin, and compare the test results with the double-stranded DNA damage test results caused by cisplatin in Example 1 (such as Figure 7 ), found that the double-stranded DNA damage caused by oxaliplatin is smaller than that caused by carboplatin, and the double-stranded DNA damage caused by carboplatin is smaller than that caused by cisplatin, indicating that the above obtained biological The sensor can respond to double-strand DNA damage and can characterize the degree of double-strand DNA damage.

Embodiment 3

[0060] According to the method in Example 1, only the double-stranded DNA grafted in step 6) is replaced with single-stranded DNA, and the biosensor obtained by the above method is used to detect the damage caused by cisplatin to the single-stranded DNA, and the device shows a resistance to cisplatin The resulting single-strand DNA damage response was similar to that of Example 1.

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Abstract

The invention discloses a nucleic acid micro-damage detection method based on a field effect transistor and a biosensor. A liquid storage tank in which a solution is stored is fixed on a field effect transistor loaded with nucleic acid molecules, and a substance capable of damaging nucleic acid is added during detection, so that the substance interacts with the nucleic acid molecules in the field effect transistor, the configuration of the nucleic acid molecules is triggered to change, and the current change of the field effect transistor is further caused. The DNA micro-damage caused by drug molecules can be detected with high sensitivity. The method is a rapid and cheap DNA damage detection method.

Description

technical field [0001] The invention belongs to the field of bioelectronics and multifunctional sensing, and in particular relates to a field-effect transistor-based nucleic acid micro-damage detection method and a biosensor. Background technique [0002] DNA is damaged by endogenous metabolites, environmental carcinogens, and genotoxic chemotherapy drugs. These DNA damages can lead to gene mutation and chromosomal damage, which may lead to cancer and tumor growth in living organisms. However, in order to limit the instability of genes, DNA damage response pathways and repair proteins exist in living body cells to remove and alleviate DNA damage. Unrepaired DNA damage may act as a pathway to promote cell death, such as apoptosis and necrosis, which are also considered effective pathways for tumor suppression. By studying how cells replace DNA repair with cell death, researchers harness the killing effects of genotoxic chemotherapy drugs on tumor cells to treat cancer. How...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/414
CPCG01N27/4145G01N27/4146
Inventor 狄重安王娟叶德楷申弘光朱道本
Owner INST OF CHEM CHINESE ACAD OF SCI
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