Construction method of mouse depression model

Abstract

The invention discloses a construction method of a mouse depression model. The method comprises the step of injecting 7-10 [mu] g of a serotonin neuron specific nerve poison into a dorsal raphe nucleus (DRN) area of a mouse. The 5, 7-DHT can be injected into the DRN of the mouse by adopting a stereotactic injection method, and the stable depression mouse model can be obtained 30 days after the nerve poison is injected. The invention provides the construction method of the mouse depression model with reduced brain serotonin content, which is characterized in that more than 90% of serotonin neurons in a DRN area of the mouse brain are accurately removed on the premise of ensuring high survival rate of animals and not influencing motor functions; and the mouse depression model simulates low serotonin content of clinical depression patients, is beneficial for scientists to explore pathogenesis of depression, and provides a research basis for screening and evaluation of different types of anti-depression drugs and treatment schemes.

Description

technical field [0001] The invention belongs to the field of pathological experiments, and relates to a method for constructing a mouse depression model, and the invention also includes the application of mice suffering from depression and the construction method thereof. Background technique [0002] Today, as the urbanization process continues to advance, the living standards of urban residents continue to improve, but the resulting problems of education, medical care, housing prices, employment, marriage and love have made the mental problems of urban people more serious, and more and more young people Depression occurs, and in recent years, reports of suicides related to depression have emerged in an endless stream. The latest data from the World Health Organization shows that there are about 54 million patients with depression in China; in China, it costs 7.8 billion U.S. dollars every year for absenteeism, medical treatment and funeral expenses caused by depression. ...

AI Technical Summary

Problems solved by technology

All of the above stress events can induce depressive behavior in mice; however, the modeling of stress-depressive models requires a lot of work and takes a long time. Once the stressful environment is removed, the depressive behavior will also disappear.

②Anatomical operation modeling: olfactory bulb resection is used to simulate depression caused by chronic corticosterone treatment, but the animal mortality rate in the experiment is high, which requires high experimental techniques; the middle cerebral artery occlusion method is used to simulate clinical stroke patients due to limb paralysis Depressed state caused by movement restriction, but movement restriction of model animals will affect the evaluation of depressive behavior; ③ drug modeling: glucocorticoids, reserpine, and norepinephrine can be used to induce depression, and the application of drug-induced models is the most It is widely used, but it cannot simulate and restore the natural pathogenesis of human depression; ④Genetic engineering modeling: The more classic ones currently used are the Flinders sensitive rat line (FSL) rat model and the Wistar-Kyoto (WKY) rat model. There are few transgenic models in mice; transgenic models mimic some of the characteristics of human depression and have certain limitations

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