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Artificial cultivation method of Chinese saprophytic boletus

A technology of artificial cultivation and boletus, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problem of long-term growth, it takes 2 to 3 years, or even 6 to 7 years for fruiting, and no Chinese saprophytic boletus is artificially produced. Cultivation, difficulty in artificial cultivation, etc.

Active Publication Date: 2021-09-07
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As we all know, most boletes belong to mycorrhizal fungi, and it is difficult to realize artificial cultivation; currently, there are no reports of stable artificial cultivation of other boletus except for Dictyostelium phalanx, which has achieved industrialized annual stable cultivation and production.
In addition, mycorrhizal fungi such as pine milk mushrooms and truffles have succeeded in semi-artificially simulated cultivation methods through the synthesis of mycorrhizal seedlings, but it takes a long time, 2 to 3 years, or even 6 to 7 years to produce mushrooms
At present, there is no relevant report on the artificial cultivation of Boletus sinensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for artificially cultivating Chinese saprophytic boletus, comprising the steps of:

[0019] (1) Cultivation medium preparation: Mix 40kg of sawdust, 20kg of cottonseed hulls, 20kg of corncobs, 10kg of bran, and 10kg of cornmeal, dissolve 100g of magnesium sulfate, potassium dihydrogen phosphate and 50g of sucrose in water, and then spray them evenly Stockpile and mix evenly, add water and stir to make the water content of the cultivation material reach 60-65%, and obtain the cultivation substrate of Boletus sinensis;

[0020] (2) Bagging and sterilization: divide the cultivation substrate obtained in step (1) into bacteria bags, each bag is 1-1.2kg, with moderate and even tightness, punch holes, cover the mouth of the bag with a plastic ring, and cover it with a waterproof plastic cover Seal, use high temperature, high pressure sterilization, temperature 121 ℃, sterilization time 90 ~ 120min, cool down for later use;

[0021] (3) Inoculation and bacterial stic...

Embodiment 2

[0026] A method for artificially cultivating Chinese saprophytic boletus, comprising the steps of:

[0027] (1) Cultivation medium preparation: Mix 30kg of sawdust, 15kg of cottonseed hulls, 10kg of corncobs, 8kg of bran, and 5kg of cornmeal, and dissolve 50g of magnesium sulfate, potassium dihydrogen phosphate, and sucrose in water, and then spray them into the material evenly. Pile and mix evenly, add water and stir to make the water content of the cultivation material reach 60-65%, and obtain the cultivation substrate of Boletus sinensis;

[0028] (2) Bagging and sterilization: divide the cultivation substrate obtained in step (1) into bacteria bags, each bag is 1-1.2kg, with moderate and even tightness, punch holes, cover the mouth of the bag with a plastic ring, and cover it with a waterproof plastic cover Seal, use high temperature, high pressure sterilization, temperature 121 ℃, sterilization time 90 ~ 120min, cool down for later use;

[0029] (3) Inoculation and bacte...

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PUM

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Abstract

The invention belongs to the technical field of fungus cultivation, and discloses an artificial cultivation method of Chinese saprophytic boletus. The method comprises the following steps: (1) preparation of a culture medium: uniformly mixing wood chips, cottonseed hulls, corncobs, bran and corn flour to obtain a culture material, and dissolving magnesium sulfate, monopotassium phosphate and cane sugar in water according to 0.05-0.1% of the mass of the culture material to obtain the culture medium; (2) bagging and sterilizing: sub-packaging the culture medium into fungus bags, carrying out sterilizing and cooling for later use; (3) inoculation and mushroom stick culture: inoculating solid strains of the Chinese saprophytic boletus strains into the sterilized culture medium, and culturing in a dark place; (4) preparation of a soil covering material and soil covering culture; (5) fruiting management: 10-15 days after soil covering, when a large number of golden yellow hyphae appear on the surface of a soil covering layer and primordia are formed, carrying out fruiting management; and (6) harvesting. The method is low in cost and short in cultivation period, the produced mushrooms are high in yield and good in quality, and technical support is provided for germplasm resource protection, development and utilization of the Chinese saprophytic boletus.

Description

technical field [0001] The invention relates to the technical field of fungal cultivation, in particular to a method for artificial cultivation of boletus saprophyticus. Background technique [0002] Chinese saprophytic boletus (Buchwaldoboletus xylophilus) belongs to the family Boletaceae and belongs to the genus Saprophytic boletus. It is a rare boletus in tropical regions of my country. The wild fruiting bodies of Boletus sinensis are only found in Xishuangbanna Prefecture of Yunnan Province, Haikou City of Hainan Province, and Hong Kong in China; they are distributed in Sri Lanka, Malaysia, and the Philippines abroad. At present, the wild resources of Boletus saprophyticus are scarce, and the yield is low, which is an endangered species. Yunnan Institute of Tropical Crops Science and Hainan Medical College conducted molecular identification and biological characteristics research on wild Boletus sinensis collected from Xishuangbanna Prefecture, Yunnan Province and Haiko...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 张春霞杨天伟许欣景何明霞方艺伟高锋刘静王文兵
Owner YUNNAN INST OF TROPICAL CROPS
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