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Molecular marker for detection or curative effect evaluation of liver cancer and application

A technology of molecular markers and curative effect evaluation, applied in drug combinations, organic active ingredients, biochemical equipment and methods, etc., can solve the problems of high incidence, lack of HCC imaging features, unsatisfactory prognosis of liver cancer, etc., to achieve Excellent specificity and improved detection accuracy

Active Publication Date: 2021-09-03
东莞市寮步医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although most HCCs have characteristic features on imaging, approximately 10% of tumors (up to 30% in tumors up to 1-2 cm in diameter) have an atypical appearance and lack the imaging features of HCC
Furthermore, the prognosis after curative resection of liver cancer remains unsatisfactory due to the high incidence of postoperative recurrence

Method used

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  • Molecular marker for detection or curative effect evaluation of liver cancer and application
  • Molecular marker for detection or curative effect evaluation of liver cancer and application
  • Molecular marker for detection or curative effect evaluation of liver cancer and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] This example shows the expression of circPNN (hsa_circ_0101802), circFCHO2 (hsa_circ_0002490) and circPTPN12 (hsa_circ_0005197) in human liver cancer cell lines and human normal liver cell lines:

[0065] Human normal liver cell line HL-7702 cells use RPMI-1640 medium + 10% FBS fetal bovine serum (FBS); while Huh-7 uses DMEM medium + 10% FBS; SK-HEP-1 uses a typical culture from the Chinese Academy of Sciences Special culture medium purchased from the Cell Bank (Shanghai) of the Committee for the Preservation of Animals. The above cells were all cultured in a cell incubator at 37° C. with a CO2 saturation humidity of 5%, and grew adherently.

[0066] (1) Cell recovery

[0067] From a liquid nitrogen tank or a -80°C ultra-low temperature refrigerator, quickly shake back and forth in a 37°C water bath to melt the cells quickly. Use a 1ml pipette gun to transfer the cell suspension in the cryopreservation tube into a 15ml centrifuge tube, blow and mix evenly, and centrif...

Embodiment 2

[0103] This example is the effect of silencing circPNN (hsa_circ_0101802) in liver cancer cell SK-HEP-1 on cell biological behavior:

[0104] (1) Experimental grouping

[0105] ①siRNA-1 group: circPNN-siRNA-1 (sequences are SEQ ID NO:10 and SEQ ID NO:11) / lentivirus complex was added during transfection. ②siRNA-2 group: circPNN-siRNA-2 (sequences are SEQ ID NO: 12 and SEQ ID NO: 13) / lentivirus complex was added during transfection. ③Blank control group: liver cancer cells without any intervention measures.

[0106] (2) Cell transfection

[0107] Take SK-HEP-1 liver cancer cells in good growth state and logarithmic growth phase, digest with trypsin, centrifuge, resuspend in culture medium and count, adjust the cell concentration to 1.0×10 5 Inoculate into 6-well plates at the density of cells / ml and place in CO 2 Cultivate in an incubator until the cell density reaches 70%-80%; discard the old medium and wash the cells twice with PBS. Use the complete medium to dilute the 2...

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Abstract

The invention discloses a molecular marker for detection or curative effect evaluation of liver cancer and application. The molecular marker comprises at least one of hsa_circ_0002490, hsa_circ_0005197 and hsa_circ_0101802, the nucleotide sequence of the hsa_circ_0002490 is as shown in SEQ ID NO: 1, the nucleotide sequence of the hsa_circ_0005197 is as shown in SEQ ID NO: 2, the nucleotide sequence of the hsa_circ_0101802 is as shown in SEQ ID NO: 3. The molecular marker has a correlation relationship with the liver cancer, so that auxiliary diagnosis of the liver cancer can be quickly, simply and accurately realized, and a basis is provided for liver cancer diagnosis and curative effect evaluation index selection; and in addition, the molecular marker can also be used as a liver cancer drug treatment target.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker for detecting or evaluating the efficacy of liver cancer and its application. Background technique [0002] Primary liver cancer (PLC) is listed as the seventh most common cancer in the world, and hepatocellular carcinoma (HCC) caused by malignant transformation of liver cells accounts for about 75% of primary liver cancer (PLC). Intrahepatic cholangiocarcinoma, another major PLC, originates from cholangiocytes and accounts for 12–15% of liver malignancies. Due to the aggressive nature of these hepatic malignancies, primary liver cancer has become the fourth most common cause of cancer death. According to statistics, more than 90% of HCC cases occur in the context of chronic liver disease, and liver cirrhosis caused by any etiology is a high risk factor for HCC, including chronic alcohol consumption, diabetes or obesity-related non-alcoholic steatohepatitis (NASH)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113A61K31/713A61P35/00
CPCC12Q1/6886A61K31/713A61P35/00C12Q2600/178C12N15/113C12N2310/532C12Q2600/158C12N2310/14C12N2320/32Y02A50/30
Inventor 缪辉来梁浩凡叶志荣程海兵杨子毅
Owner 东莞市寮步医院
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