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Water-soluble near-infrared two-region fluorescent tracing developer for joint bacterial infection

A fluorescent tracking and near-infrared technology, applied in the field of biomedicine, can solve the problems of inability to quickly and accurately locate the infection foci, difficult to locate and target therapy, delayed targeted medication, etc., and achieves good market application prospects and mild conditions. , the effect of low cost

Inactive Publication Date: 2019-12-13
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Clinically, joint bacterial infection is often very hidden, difficult to locate and target treatment
The traditional diagnostic method of bacterial culture in vitro often leads to the delay of targeted medication, and cannot quickly and accurately locate the focus of infection
A number of studies have proved that ordinary fluorescent dyes can be used to biomark bacteria to detect infection foci in vivo, but no research has been able to prepare near-infrared second-region materials with high fluorescence intensity and photostability, which can be highly effective against joint bacterial infections. Specific and highly sensitive real-time tracking and in vivo imaging

Method used

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  • Water-soluble near-infrared two-region fluorescent tracing developer for joint bacterial infection
  • Water-soluble near-infrared two-region fluorescent tracing developer for joint bacterial infection
  • Water-soluble near-infrared two-region fluorescent tracing developer for joint bacterial infection

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Embodiment 1

[0056] This embodiment involves the following steps:

[0057] 1) In the reaction system of RNase A and NaOH, use Pb(OAc) 2 ·3H 2 O and Na 2 S was used as raw material, and RNase A-PbS quantum dots were prepared by high temperature synthesis method of microwave reactor.

[0058] Specifically: mix 500ul of 10mM lead acetate solution and 500ul of 50mg / ml RNase A solution, add 50ul of 1M NaOH solution to ensure that the pH of the system is in the range of 9 to 11; then add 10mM freshly prepared Na 2 S solution 50ul, the near-infrared second region nanomaterial RNase A-PbS quantum dots were prepared in a microwave reactor. The optimal parameters were: power 30W, temperature 70℃, time 30s.

[0059] 2) The product was concentrated in an ultrafiltration tube, washed with PBS repeatedly and ultrafiltered until the solution was nearly neutral, and finally resuspended to 1 ml of the system.

[0060] Specifically: move the RNase A-PbS solution obtained in step 1) to a 10KD ultrafiltra...

Embodiment 2

[0072] 1) In the reaction system of RNase A and NaOH, use Pb(OAc) 2 ·3H 2 O and Na 2 S was used as raw material, and RNase A-PbS quantum dots were prepared by high temperature synthesis method of microwave reactor.

[0073] Specifically: mix 500ul of 10mM lead acetate solution and 500ul of 50mg / ml RNase A solution, add 50ul of 1M NaOH solution to ensure that the pH of the system is in the range of 9 to 11; then add 10mM freshly prepared Na 2 S solution 100ul was used to prepare near-infrared second-region nanomaterial RNase A-PbS quantum dots in a microwave reactor. The optimal parameters were: power 30W, temperature 75°C, and time 30s.

[0074] 2) The product is placed in an ultrafiltration tube to concentrate, and repeatedly washed with PBS and ultrafiltered until the solution is neutral, and finally resuspended to a 1ml system;

[0075]Specifically: move the RNase A-PbS solution obtained in step 1) to a 10KD ultrafiltration tube, filter and concentrate it once in a centr...

Embodiment 3

[0084] 1) In the reaction system of RNase A and NaOH, use Pb(OAc) 2 ·3H 2 O and Na 2 S was used as raw material, and RNase A-PbS quantum dots were prepared by high temperature synthesis method of microwave reactor.

[0085] Specifically: mix 500ul of 10mM lead acetate solution and 500ul of 50mg / ml RNase A solution, add 50ul of 1M NaOH solution to ensure that the pH of the system is in the range of 9 to 11; then add 10mM freshly prepared Na 2 S solution 50ul, the near-infrared second region nanomaterial RNase A-PbS quantum dots were prepared in a microwave reactor. The optimal parameters were: power 30W, temperature 70℃, time 30s.

[0086] 2) The product was concentrated in an ultrafiltration tube, washed with PBS repeatedly and ultrafiltered until the solution became neutral, and finally resuspended to 1 ml of the system.

[0087] Specifically: move the RNase A-PbS solution obtained in step 1) to a 10KD ultrafiltration tube, filter and concentrate it once in a centrifuge at...

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Abstract

The invention discloses a water-soluble near-infrared two-region fluorescent tracing developer for joint bacterial infection. The method comprises the following steps: in a reaction system of RNase Aand NaOH, taking Pb(OAc)2.3H2O and Na2S as raw materials, preparing RNase A-PbS quantum dots by utilizing a microwave reaction high-temperature synthesis method, and crosslinking amino-beta-cyclodextrin or CGGG-UBI29-41 by utilizing an EDC-NHS or EDC-SMCC system, thereby obtaining the product. The fluorescent tracing developer has the characteristics of high fluorescence intensity, high quantum yield, light stability, wide excitation spectrum, narrow emission spectrum and the like, and can be bound with bacteria in a targeting manner; due to the functions of near-infrared two-region imaging and bacterial infection focus positioning, the fluorescent tracing developer can perform rapid detection, real-time tracing and living body imaging on clinically hidden joint infection, and auxiliary diagnosis of the joint infection is achieved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a water-soluble near-infrared second-region fluorescent tracer developer for joint bacterial infection. Background technique [0002] Near-infrared second region (NIR-II, 1000-1400nm, photon energy 1.24-0.89eV) quantum dots are a new class of nanomaterials that can excite fluorescence and image. Compared with traditional fluorescent dyes or near-infrared first region materials, quantum dots have high fluorescence Intensity, high quantum yield, good photostability, different wavelengths of fluorescence under the same wavelength excitation, safe and non-toxic. However, its application as a fluorescent tracer contrast agent in the biomedical field, especially in bacterial infections, has not been fully exploited. Since near-infrared second-region quantum dots have many of the above advantages over other types of developers, especially in vivo tracking and imaging, they have great applicati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K41/00A61P31/04
CPCA61K41/0052A61K49/0067A61P31/04
Inventor 陈俊冯思嘉陈蓦杨以萌陈世益
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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