Intelligent adjustable self-heating inoculator for microbial experiments
An adjustable and microbiological technology, applied in the field of microbiological experiments, can solve the problems that liquids are prone to bursting, danger, and high temperature of alcohol lamps, and achieve the effects of avoiding splashing of bacteria-containing liquids, visualizing temperature changes, and avoiding mistakes.
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Embodiment 1
[0040] (1) Put the solid slant medium cultured with Escherichia coli and the triangular flask with liquid medium in the left hand, turn on the heating switch of the inoculation loop and the switch of the display screen, and use the ring finger and little finger of the right hand to hold the tampon and triangular flask on the slant Bottle of tampon. Stop heating when the temperature of the display screen reaches 900°C; wait for the temperature of the inoculation loop to drop to room temperature, take the bacteria on the slope, put the bacteria into the liquid medium of the Erlenmeyer flask, and recover the tampons of the flask and the slope. The Erlenmeyer flask was shaken at 37°C for 12 hours on a shaker at 200 rpm.
[0041] (2) Separation by dashes. Turn on the heating switch of the inoculation loop and the switch of the display screen, and stop heating when the temperature of the display screen 12 reaches 300°C. Open the bacterial solution that has been cultivated on the s...
Embodiment 2
[0046] (1) Put the solid slant medium cultured with Escherichia coli into the left hand and separate by marking. Turn on the heating switch of the inoculation loop and the switch of the display screen, and stop heating when the temperature of the display screen 12 reaches 300° C., and wait until the temperature of the inoculation loop drops to room temperature.
[0047] (2) Shift the gear of push button 8 to third gear, put the inoculation loop part into the solid culture medium of Escherichia coli, and obtain the agar containing Escherichia coli. Then, switch the push button 8 gears to the first gear, draw a line continuously on the flat plate of the solid medium, cover the culture dish after marking, and invert the culture. This experiment was repeated three times.
[0048] (3) Repeat the above steps (1)-(2) three times using a common inoculation loop.
[0049] (4) After culturing in a constant temperature incubator at 37°C for 12-24 hours, discontinuous single colonies ca...
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