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Extraction and purification method of high-bioavailability teasel root saponin VI

A technology of Dipsacus saponin and purification method, which is applied in the field of extraction and purification of Dipsacus saponin VI, which can solve the problems of high cost, complex extraction and purification process, and low extraction rate

Active Publication Date: 2021-09-03
GUANGZHOU BOJI MEDICINE SERVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above extraction and purification process is relatively complicated, the practical reagents include strong corrosive reagents, there are many production steps, and the cost is relatively high in industrial production. In the process of traditional Chinese medicine extraction, the more steps, the more technical points need to be controlled. Extraction The rate is relatively lower

Method used

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  • Extraction and purification method of high-bioavailability teasel root saponin VI
  • Extraction and purification method of high-bioavailability teasel root saponin VI
  • Extraction and purification method of high-bioavailability teasel root saponin VI

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Weigh 0.5kg of medicinal materials, add 95% EtOH 2L, stir and extract at 50°C for 2 hours, extract with suction and filter the liquid for later use, use 2L of 95% EtOH for medicinal residues, stir and extract for 2 hours, extract with suction and filter, remove medicinal residues, The two extracts were mixed and concentrated at 70°C to obtain 0.133 kg of dry paste of the sample. The dry paste was dissolved with 2L of purified water and stirred at 70°C, cooled to room temperature with tap water, centrifuged at 4000rpm for 10min, and 100g of NaCl was added. , 300ml of petroleum ether, stirred with a magnetic stirrer at 35°C to form a precipitate for 24h, filtered the precipitate with suction, washed with 400ml of 5% NaCl solution, and 400ml of purified water in sequence, and dried the sample in vacuum (70°C, -0.1MPa) to obtain 26.4g. Add 60ml of 95% ethanol to the sample and stir to dissolve in a 70°C water bath. After the dissolution, concentrate and dry in a 70°C water b...

Embodiment 2

[0028]Weigh 0.5kg of medicinal materials, add 95% EtOH 2L, stir and extract at 50°C for 2 hours, extract with suction and filter the liquid for later use, use 2L of 95% EtOH for medicinal residues, stir and extract for 2 hours, extract with suction and filter, remove medicinal residues, The two extracts were mixed and concentrated at 70°C to obtain 0.136 kg of dry paste of the sample. The dry paste was dissolved with 2L of purified water and stirred at 70°C, cooled to room temperature with tap water, centrifuged at 4000rpm for 10min, and 100g of NaCl was added. , 300ml of n-hexane, stirred with a magnetic stirrer at 35°C to form a precipitate for 24h, filtered the precipitate with suction, washed with 400ml of 5% NaCl solution, and 400ml of purified water, and dried the sample in vacuum (70°C, -0.1MPa) to obtain 25.9g. Add 60ml of 95% ethanol to the sample and stir to dissolve in a 70°C water bath. After the dissolution, concentrate and dry in a 70°C water bath, add 260ml of pu...

Embodiment 3

[0030] Weigh 0.5kg of medicinal materials, add 95% EtOH: ethyl acetate = 8:2 2L, stir and extract at 50°C for 2 hours, filter the extract with suction, and reserve the medicinal solution, use 95% EtOH: ethyl acetate = 8:2 for medicinal residues , 2L, stirred and extracted for 2h, the extract was filtered with suction, the dregs were removed, the two extracts were mixed, and concentrated at 70°C to obtain a sample dry paste 0.137kg, this dry paste was dissolved with 2L of purified water, stirred at 70°C, and used Cool the tap water to room temperature, centrifuge the liquid medicine at 4000rpm for 10min, add 100g of NaCl and 300ml of petroleum ether, stir with a magnetic stirrer at 35°C to form a precipitate for 24h, filter the precipitate with suction, wash with 400ml of 5% NaCl solution, and 400ml of purified water in sequence , the sample was vacuum-dried (70°C, -0.1MPa) to obtain 28.0g, and 60ml of 95% ethanol was added to the sample and stirred and dissolved in a 70°C water...

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Abstract

The invention discloses an extraction and purification method of high-bioavailability teasel root saponin VI. The method comprises the following steps: extracting teasel root medicinal material by using ethanol, recovering a solvent from an extracting solution under reduced pressure, adding a NaCl solution for dissolving, adding an emulsifier, keeping the temperature, stirring and separating out precipitate, filtering the precipitate, dissolving the obtained precipitate by using ethanol, recovering the solvent, dissolving by using the NaCl solution, adding the emulsifier, keeping the temperature, stirring and separating out the precipitate, filtering the precipitate, and drying to obtain the high-purity teasel saponin VI. The raw materials are rich in source and low in price, toxic reagents are prevented from being used in the whole preparation process, safety and low toxicity are achieved, the preparation process is simple, the separation and purification efficiency is high, the purity and yield of the product are greatly improved, and the obtained teasel root saponin VI is high in bioavailability, good in druggability, suitable for industrial production and easy to apply and popularize.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, in particular to a method for extracting and purifying Dipsacus saponin VI with high bioavailability. Background technique [0002] Dipsacus is the dry root of Dipsacus asperoides.Y.Cheng et T.M.Ai. It was first recorded in "Shen Nong's Materia Medica", listed as the top grade, and it is recorded that it is "bitter in taste, slightly warm, mainly for typhoid fever, tonic for insufficiency, gold sores, carbuncles, folds, joints and bones, and difficult for women to breastfeed. Long-term use can benefit energy. 一 The name is Longdou, and the other is Zhe." Li Shizhen said, "Dusan, Zhe, and Bone are all named after Gong." From its name, it can be seen that Dipsacan has been an important medicine for orthopedics since ancient times. Today's Pharmacopoeia records: "bitter, pungent, slightly warm. Returns to the liver and kidney meridian. Tonifies the liver and kidney, strengthens the bones ...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07J63/00C07H1/08A61K36/185
CPCC07H15/256C07J63/008C07H1/08A61K36/185Y02A50/30
Inventor 马仁强徐鸿王廷春方哲周清马艺华
Owner GUANGZHOU BOJI MEDICINE SERVICES
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