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Composition for improving atopic dermatitis and skin wrinkles, containing lactobacillus-fermented thyme extract as active ingredient

A technology for atopic dermatitis and skin wrinkles, which is applied in the field of compositions for improving atopic dermatitis and skin wrinkles, and can solve problems such as lowering immunity, high therapeutic effect of atopic dermatitis, and growth retardation

Active Publication Date: 2021-08-31
SD BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, as the treatment of atopic dermatitis, steroid preparations that inhibit inflammatory response and cytokine production are mainly used, but long-term administration may induce skin atrophy or growth retardation Sexual and other side effects, so the use of non-steroidal preparations has recently increased
However, non-steroidal preparations also have symptoms such as erythema, itching, edema, ulceration and lichenification, and various side effects such as reduced immunity, so it is difficult to fundamentally treat atopic dermatitis (Arellano FM et al., JInvest Dermatol.2007Apr ;127(4):808-16.)
[0009]Therefore, efforts have been made to discover natural substances that have a significant therapeutic effect on atopic dermatitis, but so far, a single material that has a high therapeutic effect on atopic dermatitis has not been developed. In fact, as a new attempt, it is necessary to conduct research on the direction of improving the therapeutic effect of atopic dermatitis on natural products known to have atopic therapeutic effects and high stability.

Method used

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  • Composition for improving atopic dermatitis and skin wrinkles, containing lactobacillus-fermented thyme extract as active ingredient
  • Composition for improving atopic dermatitis and skin wrinkles, containing lactobacillus-fermented thyme extract as active ingredient
  • Composition for improving atopic dermatitis and skin wrinkles, containing lactobacillus-fermented thyme extract as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1. The preparation of lactic acid bacteria fermented thyme extract

[0064] Wash thyme under running water to remove impurities. After adding 6 L of purified water to 600 g of thyme, the mixture was stirred at room temperature for 3 days to extract. The process was performed using 3-batch. After collecting the obtained extract, it concentrated under reduced pressure at 40 degreeC or less, and obtained the concentrated extract of the juice form. After 1 mg of the concentrated thyme extract was dissolved in 10 mL of purified water, Lactobacillus plantarum (Lactobacilus plantarum) strain was inoculated and cultured at 55°C for 24 hours.

[0065] The culture was sterilized at 121° C. for 15 minutes to inactivate the strain, and then centrifuged to collect only the supernatant. The thyme extract fermented by lactic acid bacteria thus obtained was concentrated under reduced pressure at 55° C. using a reduced-pressure concentrator (Rotary evaporator N-1000, EYE...

experiment example 1

[0067] Experimental example 1. Cytotoxicity test

[0068] MTT colorimetric assay was used to confirm the effect of the Thyme extract fermented by lactic acid bacteria prepared in Example 1 on the growth of human keratinocytes (HaCaT, Human immortalized keratinocyte).

[0069] First, use a special medium containing 10% fetal bovine serum (fetal bovine serum, FBS (Cambrex)), that is, Dulbeccos modified Eagles medium (DMEM) medium, to 1×10 5 Concentration After inoculating human keratinocytes into 24-well (well) cell culture dishes, at 37°C, 5% CO 2 Incubate for 24 hours under humid conditions. Afterwards, the Thyme extract fermented by lactic acid bacteria prepared in Example 1, which was removed from the medium and diluted with serum-free DMEM medium, was treated at concentrations (10 and 100 μg / mL) and cultured for 24 hours. After 24 hours, the cells were treated with 1 mg / mL of MTT reagent, and 2 hours later, formazan produced in the cells by the MTT treatment was dissol...

experiment example 2

[0071] Experimental example 2. Inhibition of expression of atopic factor TARC

[0072] Human keratinocytes (HaCaT5×10 5 / well) after culturing in FBS DMEM medium for 24 hours, dilute the lactic acid bacteria fermented Thyme extract (10 and ) and TNF-a and IFN-r (10ng / mL) were simultaneously treated for additional culture. After 24 hours, the supernatant of each well (well) was quantified using the human thymus activation-regulated chemokine ELISA kit (human TARC ELISA kit) according to the manufacturer's protocol.

[0073] As a result, such as figure 2 As shown, it was confirmed that the amount of TARC production due to the overexpression of TNF-a and IFN-r by the thyme extract fermented by lactic acid bacteria of the present invention was significantly suppressed.

[0074] Specifically, compared with the tacrolimus (10 μg / mL) treatment group used as a positive control group, the lactic acid bacteria fermented thyme extract treatment group (10 μg / mL) was measured to redu...

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Abstract

The present invention relates to a composition for improving atopic dermatitis and skin wrinkles, which contains a lactobacillus-fermented thyme extract as an active ingredient. The lactic acid bacteria fermented thyme extract of the present invention effectively inhibits the production of TARC and MDC proteins as atopic factors, and inhibits the production of inflammatory cytokines such as IL-6, thereby being very effective for atopic immunity or inflammatory response, and can inhibit the production of MMP-1 protein and promote the production of type 1 procollagen. Therefore, the composition can be usefully used as a cosmetic, a food and a pharmaceutical composition for improving atopic dermatitis and skin wrinkles. Furthermore, the lactobacillus-fermented thyme extract is a fermented extract obtained by fermenting a natural thyme extract with lactic acid bacteria, and is not only very safe to a human body, but also very excellent in safety.

Description

technical field [0001] The present invention relates to a composition for improving atopic dermatitis and skin wrinkles with thyme extract as an active ingredient, more specifically, a composition for improving atopic dermatitis containing lactic acid bacteria fermented thyme extract as an active ingredient. and skin wrinkles. Background technique [0002] Atopic dermatitis is a chronic relapsing pruritic dermatitis that often occurs in infancy and is a disease accompanied by itching, dry skin or characteristic eczema in patients or family members. The typical symptoms of atopic dermatitis appear on the hands, scalp, face, neck, elbows, knees, etc. The skin is very dry, itchy and inflamed, and scales fall off, and the skin thickens and wrinkles deepen when scratching is severe of lichenification. [0003] The etiology of atopic dermatitis has not been clarified so far, but abnormal increase of IgE, decreased number and function of T cells responsible for the central role o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/08A61K36/53A61P17/00A61P29/00A23L33/105
CPCA61K8/9789A61Q19/08A61K36/53A61P17/00A61P29/00A23L33/105A61K2800/85A61K2236/19A23V2002/00A23V2200/318A23V2250/21A61Q19/00A61K35/747A23L29/00A23L29/065
Inventor 朴卨雄黃恩宣朴相勇杨贞恩徐秀儿
Owner SD BIOTECH
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