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Colloidal gold card for detecting pesticide residues as well as preparation method and application of colloidal gold card

A colloidal gold card and pesticide residue technology, applied in the field of pesticide detection, can solve the problems of timely and rapid detection of large quantities of samples or temporary samples, poor accuracy and anti-interference, expensive instruments, etc., to achieve multiple protection antigens, results Accurate, the effect of improving binding efficiency

Pending Publication Date: 2021-08-31
WUXI ZODOLABS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main methods for the detection of pesticide residues still rely on large modern analytical instruments such as liquid chromatography, gas chromatography, liquid chromatography-tandem mass spectrometry, gas chromatography-mass spectrometry, etc. Although these instruments have high detection sensitivity, they are expensive. Expensive, cumbersome operation, and long cycle, it is difficult to meet the timely and rapid detection of large quantities of samples or temporary samples. Therefore, people are committed to developing methods that can detect pesticide residues quickly, accurately, conveniently, and at low cost
[0004] CN101319997 discloses a card suitable for rapid detection of pesticide residues in tea and a detection method thereof. The card is composed of an immobilized enzyme sheet containing a stabilizer and a color development sheet, which is easy to operate, short in detection time, and can quickly and effectively identify pesticides Residual tea leaves exceeding the standard, but poor accuracy and anti-interference
[0005] CN105181949A discloses a heterologous competition immunochromatographic gold standard test strip suitable for rapid detection of multiple residues of imidacloprid and other three chlorinated nicotine pesticides in tea. The test strip includes a liner, a sample pad, a gold standard Anti-binding pad, nitrocellulose membrane, absorbent pad, gold-labeled primary antibody binding pad coated with colloidal gold-labeled imidacloprid-specific mouse monoclonal antibody, nitrocellulose membrane coated with chlorothialine for heterologous competition Or the detection line of clothianidin artificial antigen and the quality control line coated with rabbit anti-mouse IgG can quickly detect the results of pesticide residues in tea, but the anti-interference is poor, and the color development is easily affected by tea polyphenols and flavonoids in tea. substances, pigments or minerals

Method used

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  • Colloidal gold card for detecting pesticide residues as well as preparation method and application of colloidal gold card
  • Colloidal gold card for detecting pesticide residues as well as preparation method and application of colloidal gold card
  • Colloidal gold card for detecting pesticide residues as well as preparation method and application of colloidal gold card

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] This embodiment provides a colloidal gold card for detecting pesticides, the structural schematic diagram of the colloidal gold card is as follows figure 1 with figure 2 shown.

[0079] The preparation method of described colloidal gold card comprises the following steps:

[0080] (1) use trisodium citrate to reduce chloroauric acid to prepare colloidal gold solution;

[0081] (2) Use 0.2M K 2 CO 3 The solution adjusts the pH of the colloidal gold solution, and then the colloidal gold solution is mixed with the imidacloprid antibody in a ratio of 1mL:6 μg for labeling to obtain the colloidal gold-labeled imidacloprid antibody;

[0082] (3) Use a phosphate buffer solution containing 0.01MpH7.4, 3% trehalose and 0.035% polyoxyethylene lauryl ether (Brij58) to scratch the nitrocellulose membrane Membrane, with a volume of 0.9 μL / cm, was used to make detection lines and quality control lines, and the detection line was coated with imidacloprid antigen, and the quality c...

Embodiment 2

[0087] The present embodiment provides a colloidal gold card for detecting pesticides, and the preparation method of the colloidal gold card comprises the following steps:

[0088] (1) use trisodium citrate to reduce chloroauric acid to prepare colloidal gold solution;

[0089] (2) Use 0.2M K 2 CO 3 The solution adjusts the pH of the colloidal gold solution, and then the colloidal gold solution is mixed with the imidacloprid antibody in a ratio of 1mL:5 μg for labeling to obtain the colloidal gold-labeled imidacloprid antibody;

[0090] (3) Use a phosphate buffer solution containing 0.01MpH of 7.4 to draw a film on a nitrocellulose membrane with a volume of 0.75 μL / cm to make a test line and a quality control line, and coat the test line with imidacloprid antigen, Coat the quality control line with goat anti-mouse IgG, and dry at 38°C for 13 hours to obtain a nitrocellulose membrane with a detection line and a quality control line;

[0091] (4) A gold-binding gel containing...

Embodiment 3

[0095] The present embodiment provides a colloidal gold card for detecting pesticides, and the preparation method of the colloidal gold card comprises the following steps:

[0096] (1) use trisodium citrate to reduce chloroauric acid to prepare colloidal gold solution;

[0097] (2) Use 0.2M K 2 CO 3 The solution adjusts the pH of the colloidal gold solution, and then the colloidal gold solution is mixed with the imidacloprid antibody in a ratio of 1mL:5 μg for labeling to obtain the colloidal gold-labeled imidacloprid antibody;

[0098] (3) Use a phosphate buffer solution containing 0.01MpH7.4, 3% trehalose and 0.035% polyoxyethylene lauryl ether (Brij58) to scratch the nitrocellulose membrane Membrane, with a volume of 0.9 μL / cm, was used to make detection lines and quality control lines, and the detection line was coated with imidacloprid antigen, and the quality control line was coated with goat anti-mouse IgG, and dried at 38 ° C for 13 hours to obtain a detection line. ...

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Abstract

The invention relates to a colloidal gold card for detecting pesticide residues as well as a preparation method and application of the colloidal gold card. The colloidal gold card comprises a bottom plate, and a sample pad, a gold combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially lapped on the bottom plate, wherein the nitrocellulose membrane is provided with a detection line coated with a pesticide antigen and a quality control line coated with a second antibody. The gold conjugate pad contains a colloidal gold labeled pesticide antibody, cane sugar, disodium hydrogen phosphate dodecahydrate, bovine serum albumin, sodium caseinate, trehalose and polyethylene glycol. In the colloidal gold card, a gold conjugate pad contains cane sugar, disodium hydrogen phosphate dodecahydrate, bovine serum albumin, sodium caseinate, trehalose and polyethylene glycol; the colloidal gold card can effectively protect antigens and antibodies, eliminates the influence of tea polyphenols, flavonoid substances, pigments, minerals and the like in tea water on color development, and is improved in the anti-interference performance.

Description

technical field [0001] The invention belongs to the technical field of pesticide detection, and relates to a colloidal gold card for detecting pesticide residues and a preparation method and application thereof, in particular to a colloidal gold card for detecting pesticide residues in tea leaves, a preparation method and application thereof. Background technique [0002] Pesticides have the functions of controlling diseases and insect pests, removing weeds and regulating plant growth, and are widely used in agriculture, but pesticide residues on crops are also harmful to human body. Chinese people have a long history of drinking tea for thousands of years, and they are also a big exporter of tea. Therefore, pesticide residues in tea are highly valued. [0003] At present, the main methods for the detection of pesticide residues still rely on large modern analytical instruments such as liquid chromatography, gas chromatography, liquid chromatography-tandem mass spectrometry,...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/558G01N33/577G01N33/532G01N33/531G01N33/53
CPCG01N33/5308G01N33/531G01N33/532G01N33/558G01N33/577G01N33/587G01N2430/10
Inventor 游冬梁如中周丽邹晓兰李林
Owner WUXI ZODOLABS BIOTECH
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