A kind of method for measuring free fatty acid content in breast milk

Abstract

The invention discloses a method for determining the content of free fatty acids in breast milk, comprising the steps of: quantitatively dropping breast milk into the spot range fixed by a dried blood spot card, drying to make dried milk spots, and extracting the dried milk spots through a dried blood spot extraction system , analyzed by liquid chromatography-mass spectrometry, and determined by external standard method. The method of the invention uses a special card to collect dry milk spot samples from breast milk, which is convenient for transportation and storage, and uses an automatic extraction apparatus-tandem liquid chromatography-tandem mass spectrometry method (DBS-LC-MS method), which saves tedious pre-detection treatment, and does not require Expensive isotope internal standards are required to accurately determine free Omega-3 fatty acids and free Omega-6 fatty acids in breast milk. The detection method is simple, rapid, accurate, and low in detection cost, in order to develop personalized diets for mothers and infants during lactation. Intake studies provide scientific evidence.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to a method for determining the content of free fatty acids in breast milk. Background technique [0002] Breast milk is the most ideal natural food for infants. Breast milk contains 3% to 5% of fat, which is an important component of infant nutrition and can provide 45% to 60% of energy for infant growth and development. Infant central nervous system development, especially polyunsaturated fatty acids such as omega-3 fatty acids and omega-6 fatty acids. [0003] At present, the detection of free Omega-3 fatty acids and free Omega-6 fatty acids in breast milk generally uses gas chromatography-tandem mass spectrometry (GC-MS), which has the advantages of high selectivity, high specificity and high sensitivity. However, due to the GC-MS method, breast milk samples are very inconvenient during transportation, the sample amount is large, and the pretreatment steps of the detection ...

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Problems solved by technology

However, due to the GC-MS method, breast milk samples are very inconvenient during transportation, and the sample volume is large, and the pretreatment steps of the detection method are relatively cumbersome. Chemicalization, GC-MS determination, etc., and in order to eliminate the matrix effect, expensive isotope internal standards need to be added, the detection time is long, the detection cost is high, and it is difficult to apply to the health monitoring needs of large samples of lactating mothers and infants

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