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Lactic acid biosensor

A biosensor, lactic acid technology, applied in the field of biosensors, can solve problems such as low stability, high cost, complex manufacturing process, etc., achieve high biocompatibility, simplify design and manufacture, and simplify design and manufacture.

Pending Publication Date: 2021-08-27
SUZHOU ZHONGXING MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the electrochemical detection of lactate by artificial redox mediators in this type of detection, the sensitivity has been significantly improved, but the linear range and stability need to be improved
On the other hand, its complex manufacturing process, high cost, limited linear range and low stability have brought many difficulties to its application promotion and commercialization.

Method used

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  • Lactic acid biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Such as figure 1 , 2 As shown, a lactic acid biosensor includes a substrate 4 , a carbon working electrode 5 , a carbon counter electrode 3 , a reference electrode 2 , a lactic acid sensing film 6 and a biocompatible film 1 .

[0042] Wherein, the substrate 4 is a polyethylene terephthalate substrate, the reference electrode 2 is a silver / silver chloride reference electrode, and the carbon working electrode 5 is an aminated graphite printing electrode.

[0043]The lactic acid sensing film 6 is formed on the surface of the carbon working electrode 5 by chemically crosslinking the lactate oxidase that establishes the electron transfer network through a bifunctional chemical crosslinking agent, and its preparation method is as follows:

[0044] S1: Incubate 5 mg / mL lactate oxidase in PBS buffer solution containing 6 mol / L guanidine hydrochloride at 4°C for 12 hours to fully develop lactate oxidase;

[0045] S2: Add 5 mg / mL of osmium complex with free amino groups, make i...

Embodiment 2

[0058] Such as figure 1 , 2 As shown, a lactic acid biosensor includes a substrate 4 , a carbon working electrode 5 , a carbon counter electrode 3 , a reference electrode 2 , a lactic acid sensing film 6 and a biocompatible film 1 .

[0059] Wherein, the substrate 4 is a polyethylene terephthalate substrate, the reference electrode 2 is a silver / silver chloride reference electrode, and the carbon working electrode 5 is an aminated graphite printing electrode.

[0060] The lactic acid sensing film 6 is formed on the surface of the carbon working electrode 5 by chemically crosslinking the lactate oxidase that establishes the electron transfer network through a bifunctional chemical crosslinking agent, and its preparation method is as follows:

[0061] S1: Incubate 1 mg / mL lactate oxidase in PBS buffer solution containing 3 mol / L guanidine hydrochloride at 4°C for 8 hours to fully develop lactate oxidase;

[0062] S2: Add 1mg / mL copper complex with free amino group, mix it well...

Embodiment 3

[0075] Such as figure 1 , 3 As shown, a lactic acid biosensor includes a substrate 4 , a carbon working electrode 5 , a carbon counter electrode 3 , a reference electrode 2 , a lactic acid sensing film 6 and a biocompatible film 1 .

[0076] Wherein, the substrate 4 is a polyethylene terephthalate substrate, the reference electrode 2 is a silver / silver chloride reference electrode, and the carbon working electrode 5 is an aminated graphite printing electrode.

[0077] The lactic acid sensing film 6 is formed on the surface of the carbon working electrode 5 by chemically crosslinking the lactate oxidase that establishes the electron transfer network through a bifunctional chemical crosslinking agent, and its preparation method is as follows:

[0078] S1: Incubate 10 mg / mL lactate oxidase in PBS buffer solution containing 8 mol / L guanidine hydrochloride at 4°C for 24 hours to fully develop lactate oxidase;

[0079] S2: Add 10mg / mL of copper complex with free amino group, mix i...

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Abstract

The invention belongs to the technical field of biosensors, and particularly relates to a lactic acid biosensor which comprises a substrate, a carbon working electrode, a carbon counter electrode, a reference electrode and a lactic acid sensing film, wherein the carbon working electrode is an aminated graphite printing electrode, the lactic acid sensing film is formed on the surface of the carbon working electrode after lactic acid oxidase for establishing an electron transfer network is chemically crosslinked by a difunctional chemical crosslinking agent, and the lactic acid oxidase for establishing the electron transfer network is prepared by modifying electrochemically activated lactic acid oxidase by a carboxylated nano material. The electrochemical activation technology of the lactate oxidase is adopted, so that the lactate oxidase can perform direct electron exchange with the electrode, the sensitivity, accuracy, reproducibility, stability, specificity and anti-interference capability of dynamic detection of lactic acid can be improved, the service life of the implantable continuous lactic acid monitoring system is prolonged, and meanwhile, the cost of the lactic acid biosensor is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to a lactic acid biosensor. Background technique [0002] Lactic acid is the final product of glucose in the process of anaerobic metabolism in the human body. The content of lactic acid in the blood is directly related to some diseases. Its accurate monitoring is of great significance for the health assessment of critically ill patients and surgical patients. In addition, lactic acid will accumulate in the human body in a state of hypoxia or excessive fatigue. For example, the normal value of lactic acid in human blood is 1.2-2.7mmol / L, but after strenuous exercise, the lactic acid in human blood can be as high as 25mmol / L. Therefore, the content of lactic acid in body fluids and tissues effectively reflects whether the local tissues of the human body are hypoxic or whether the exercise is overloaded. If it cannot be metabolized in time, it will lead to lactic acidosis, sympt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/308G01N27/3278
Inventor 沈薇
Owner SUZHOU ZHONGXING MEDICAL TECH CO LTD
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