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A detection method for hydrogen peroxide or glucose

A detection method, glucose technology, applied in the detection field, can solve problems such as the influence of detection results, harsh storage conditions of TMB, and limitations of practical applications

Active Publication Date: 2022-05-06
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection process requires first H 2 o 2 Catalytic decomposition produces OH, which then oxidizes the substrate TMB to oxTMB; this method requires two-step reactions, which can easily affect the detection results
At the same time, the harsh storage conditions of TMB require low temperature and light protection, which further limits its practical application.

Method used

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  • A detection method for hydrogen peroxide or glucose
  • A detection method for hydrogen peroxide or glucose
  • A detection method for hydrogen peroxide or glucose

Examples

Experimental program
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Effect test

Embodiment 1

[0076] The embodiment of the detection method of hydrogen peroxide or glucose of the present invention is specifically as follows:

[0077] The preparation method of C-SPIO nano enzyme, comprises the steps:

[0078] (1) Under the protection of argon, 1 mmol iron acetylacetonate and 30 mL triethylene glycol were fully dissolved and mixed, then heated to obtain solution A;

[0079] (2) Reflux solution A at 280° C. for 30 minutes to obtain solution B;

[0080] (3) Add ethyl acetate to solution B to form a precipitate, disperse the precipitate in absolute ethanol, mix it with an aqueous solution of sodium citrate, and react in a microwave reactor at 100°C for 30 minutes to obtain sodium citrate-modified trioxide Iron nanozyme, counted as C-SPIO nanozyme.

[0081] The detection method of described hydrogen peroxide, comprises the steps:

[0082] (1) Disperse 25 μl, 0.5mM C-SPIO nanozyme in 450 μl, a pH value of 4.0 acetate buffered saline solution to obtain a C-SPIO nanozyme sus...

Embodiment 2

[0091] The embodiment of the detection method of hydrogen peroxide or glucose of the present invention is specifically as follows:

[0092] The preparation method of the C-SPIO nanozyme in this implementation is the same as the preparation method of the C-SPIO nanozyme described in Example 1.

[0093] The only difference between the hydrogen peroxide detection method described in this example and Example 1 is that in step (1), the pH value of the acetic acid buffered saline solution is 2.0.

[0094] The only difference between the glucose detection method described in this example and Example 1 is that in step (2), the pH value of the acetate buffered saline solution is 2.0.

Embodiment 3

[0096] The embodiment of the detection method of hydrogen peroxide or glucose of the present invention is specifically as follows:

[0097] The preparation method of the C-SPIO nanozyme described in this example is the same as the preparation method of the C-SPIO nanozyme described in Example 1.

[0098] The only difference between the hydrogen peroxide detection method described in this example and Example 1 is that in step (1), the pH value of the acetic acid buffered saline solution is 3.0.

[0099] The only difference between the glucose detection method described in this example and Example 1 is that in step (2), the pH value of the acetate buffered saline solution is 3.0.

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Abstract

The invention discloses a method for detecting hydrogen peroxide or glucose. The detection method includes the following steps: using the peroxidase activity of the modified iron ferric oxide nanozyme to catalyze the target object, and detecting the concentration of the target object by magnetic resonance technology; the target object is hydrogen peroxide or glucose. The invention adopts the magnetic resonance technology to detect the concentration of hydrogen peroxide or glucose, and overcomes the shortcomings of the colorimetric method in the prior art that the complicated steps affect the detection result and the chromogenic substrate TMB is not easy to preserve. At the same time, using the detection method of the present invention, the detection limit of C-SPIO nanozyme to hydrogen peroxide is as low as 3.32 μM; the detection limit of SPIO nanozyme cluster is as low as 8.78 μM for hydrogen peroxide, and the detection limit of both Both are far lower than the 15 μM permissible level of hydrogen peroxide stipulated by the US FDA. At the same time, the detection limit of C-SPIO nanozyme and SPIO nanozyme clusters for glucose is also low.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for detecting hydrogen peroxide or glucose. Background technique [0002] Hydrogen peroxide (H 2 o 2 ) as an active product of biological metabolism, participates in the catalytic reactions of various biological oxidative enzymes. By detecting H 2 o 2 Concentration can realize the monitoring of vital activities. Catalytic metabolism by glucose oxidase can produce efficient H 2 o 2 molecular. For example, a molecule of glucose in an organism can be metabolized by glucose oxidase to produce a molecule of H 2 o 2 , and then detect H 2 o 2 The content can reflect the glucose metabolism in the human body and obtain the blood glucose concentration. Therefore, a rapid, sensitive and selective H 2 o 2 Detection methods are of great significance for the prevention, diagnosis and monitoring of diabetes. [0003] Currently for H 2 o 2 The clinical det...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N24/08
CPCG01N24/08
Inventor 王志勇颜迟黄诗惠
Owner SUN YAT SEN UNIV
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