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Method for preparing fructus cannabis protein with high oil holding capacity and emulsifying activity

A technology for hemp seeds and protein is applied in the field of preparing hemp seed protein with high oil retention and emulsifying activity, which can solve the problems of high residual oil rate, protein denaturation, waste of white resources, etc. , Improve functional properties and reduce water consumption

Active Publication Date: 2021-08-20
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Squeezing is the earliest technology used to extract edible oil. It has the advantages of simple process and no pollution to the environment; Denaturation of protein in sesame meal, resulting in waste of protein resources

Method used

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  • Method for preparing fructus cannabis protein with high oil holding capacity and emulsifying activity
  • Method for preparing fructus cannabis protein with high oil holding capacity and emulsifying activity
  • Method for preparing fructus cannabis protein with high oil holding capacity and emulsifying activity

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: A kind of method for preparing hemp seed protein

[0052] A method for preparing hemp seed protein, said method comprising the following steps:

[0053] (1) Pretreatment of hemp seeds: Weigh 250g of hemp seeds, add 0.4mol / L citric acid solution with a material-to-liquid ratio of 1:1 (w / v), place it under 200W for ultrasonic treatment for 40min, and then dry it Handle and dry in an oven at 40°C (to a moisture content of 5%);

[0054] (2) Preparation of hemp seed protein: Weigh a certain amount of pulverized hemp seed slurry in the jacketed reactor, add deionized water according to the ratio of material to liquid 1:2.5, control the temperature to 45°C, and use the diluted hemp seed Adjust the pH to 10.0 with NaOH, centrifuge after the reaction (5000×g, 15min), separate the clear oil, emulsion, water phase and slag phase; collect the water phase after enzymatically extracting the hemp seed oil, adjust the pH to 5.0, and react for 1h After centrifuging for 1...

Embodiment 2

[0055] Embodiment 2: A kind of method for preparing hemp protein

[0056] (1) Pretreatment of hemp seeds: Weigh 250g of hemp seeds, add 0.2mol / L citric acid solution with a material-to-liquid ratio of 1:1 (w / v), place it under 100W for ultrasonic treatment for 60min, and then dry it Processing, drying in a 60°C oven (to a moisture content of 5%);

[0057] (2) Preparation of hemp seed protein: Weigh a certain amount of pulverized hemp seed slurry in a jacketed reactor, add deionized water according to the ratio of material to liquid 1:3.0, control the temperature to 45°C, and use the diluted hemp seed Adjust the pH to 9.0 with NaOH, centrifuge after the reaction (5000×g, 15min), separate the clear oil, emulsion, water phase and slag phase; collect the water phase after enzymatically extracting the hemp seed oil, adjust the pH to 5.0, and react for 1h After centrifuging for 15 minutes (5000 r / min), the obtained precipitate was washed three times with deionized water, adjusted t...

Embodiment 3

[0058] Embodiment 3: A kind of method for preparing hemp protein

[0059] (1) Pretreatment of hemp seeds: Weigh 250g of hemp seeds, add 0.6mol / L citric acid solution with a material-to-liquid ratio of 1:1 (w / v), place it under 300W for ultrasonic treatment for 60min, and then dry it Processing, drying in an oven at 80°C (to a moisture content of 5%);

[0060] (2) Preparation of hemp seed water phase protein: Weigh a certain amount of pulverized hemp seed slurry in the jacketed reactor, add deionized water according to the ratio of material to liquid 1:3.5, control the temperature to 45 ° C, dilute with The final NaOH is adjusted to pH 11.0, centrifuged (5000 × g, 15min) after the reaction, and the clear oil, emulsion, water phase and slag phase are separated; the water phase after the water enzymatic extraction of hemp seed oil is collected, and the pH is adjusted to 5.0. After reacting for 1 hour, centrifuge for 15 minutes (5000 r / min), wash the obtained precipitate three ti...

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Abstract

The invention discloses a method for preparing fructus cannabis protein with high oil holding capacity and emulsifying activity, and belongs to the technical field of foods. The method comprises the following steps of firstly, pretreating fructus cannabis by ultrasonic combined acid leaching; then crushing the pretreated fructus cannabis, and carrying out extracting and separating by a water substitution method so as to obtain clear oil, emulsion, water phase and residue phase; and finally, performing acid precipitation on the obtained water phase so as to obtain the fructus cannabis protein. The prepared fructus cannabis protein has the advantages that the oil holding capacity, emulsifying activity and in-vitro digestibility are better; and by utilizing the properties, the fructus cannabis protein can be well applied to the fields of foods, such as infant complementary foods, body building foods, special medical foods and cooking oil, the comprehensive utilization rate of fructus cannabis resources is improved, and the application field of the fructus cannabis protein is widened.

Description

technical field [0001] The invention relates to a method for preparing hemp seed protein with high oil retention and emulsifying activity, belonging to the technical field of food. Background technique [0002] About 90% of the world's edible vegetable oils come from palm, soybean, rapeseed and sunflower, compared with hemp oil which occupies only a small part of the edible oil market. However, hemp has a very high utilization value. Hemp oil, hemp protein, and cannabidiol have attracted more and more attention from researchers because of their excellent physiological functions and nutritional value, which has promoted the growth of the global industrial hemp market. Currently, the utilization efficiency of hemp resources is very low, especially the utilization of hemp seeds. In most production areas of my country, hemp oil is extracted by pressing, and the remaining hemp meal is discarded or used as feed and fertilizer, which leads to a serious waste of hemp seed resources...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14A23L13/40A23L33/185A23C9/152C11B1/04B01J19/10
CPCA23J1/146A23J1/14A23L13/426A23L33/185A23C9/1526C11B1/04B01J19/10A23V2002/00A23V2250/1614A23V2250/548A23V2200/14A23V2200/30A23V2200/328A23V2200/308A23V2250/5114
Inventor 张文斌王宇凡杨瑞金蒋将
Owner JIANGNAN UNIV
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