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A kind of plant lectin succ-con A for treating coronavirus infection and its application

A plant lectin, coronavirus technology, applied in plant lectin succ-ConA, plant lectin succ-ConA for treating coronavirus infection and application fields

Active Publication Date: 2022-03-22
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As the coronavirus continues to spread, its genome is also constantly mutating. Related studies have shown that the mutant strain D614G has been widely prevalent around the world. By December 2020, more than 98% of the sequences contained the D614G mutation (Korber B, Fischer W M, Gnanakaran S, et al. Tracking changes in SARS-CoV-2Spike: evidence that D614G increases infectivity of the COVID-19 virus[J]. Cell, 2020, 182(4): 812-827. e19.), in The British mutant strain and the South African mutant strain evolved on the basis of the N501Y mutant strain are currently mainly prevalent in the UK and South Africa. Although two mutant strains have been found in other countries, they have not yet formed outside the UK and South Africa. The main epidemic strains, in addition to these mutant strains, also include some mutant strains combined with other mutation sites, such as D614G+I472V, D614G+A435S, A222V+D614G, K417N+D614G, S477N+D614G, E484K+D614G, N501Y+ D614G

Method used

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  • A kind of plant lectin succ-con A for treating coronavirus infection and its application
  • A kind of plant lectin succ-con A for treating coronavirus infection and its application
  • A kind of plant lectin succ-con A for treating coronavirus infection and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1 Detection of Plant Legin on Binding Activity and Antiviral Activity of SARS-COV-2 S protein

[0087] 1, experimental materials

[0088] Lentil lectin, wheat germ gel (WGA), non-infarcting bone gemin (SSL), peanut lectin, Huappi rendering (MAL) is purchased from Wako (Japan) ; Mandalage group (DSL), succinoyl-accompanied kanosanole protein A (Succ-con a), Snowdotin (GNL), plant aggregate (PHA-E), leukocytin (PHA-L ), Derived from red kidney beans - P (PHA-M) and plant agglutinin-P (PHA-P) from Sigma Aldrich; HEK293 cell expressed SARS-COV-2 S trimer purchase Yu ACRO BIOSYSTEMS (China, Beijing); 293T cells are provided by the American Typical Culture Preservation Center (ATCC); HuH7 cells are provided by the Japanese Research Basic Resources Savings (JCRB); constructs SARS-COV-2 spike (mn908947; hcov -19 / Southafrica / Krisp-K007869 / 2020, B.1.351, EPI_ISL_860630; HCOV-19 / ENGLAND / QEUH-F56F0F / 2021, B.1.1.7, EPI_ISL_852526; HCOV-19 / Brazil / AM-991 / 2020, P .1...

Embodiment 2

[0101] Example 2 Inhibitory effect of plant aggregate on SARS-COV-2 S protein mutant and SARS-COV-2 epidemic mutant infection

[0102] In this example, various plant aggregates are detected in inhibitory effects of SARS-COV-2 S protein mutant and SARS-COV-2 epidemic mutant infection, including British epidemic strains B.1.1.7. , South Africa epidemic strain B.1.351, Brazil PET P.1, Combination mutant strain D614G + I435S, Combination mutant strain D614G + A435S, A222V, K417N, S477N, E484K, N501Y, combined mutant strains A222V + D614G, combined mutation Strain K417N + D614G, combined mutant strain S477N + D614G, combined mutant plant E484K + D614G, combined mutant strain N501Y + D614G and other mutants;

[0103] The agglutin is mainly directed to the glycosyl portion of the glycoprotein, in the present embodiment, the inventors have studied whether the change in the saccharification site of the SARS-COV-2 s protein affects the neutralization of the concentrate, and constructs 28 An...

Embodiment 3

[0113] Example 3 Detection of blood cell agglutination activity and cytotoxicity of plant lectin

[0114] Since many natural lectins cause blood cell agglutination, the inventors in this example have studied blood cell agglutination activity of different plant agglutines, and the cytotoxicity of HUH7 and 293T cells was studied.

[0115] 1. Detection of blood cell agglutination activity of plant lectin

[0116] The blood cell agglutination activity of plant lectin was detected by a chicken blood specimen, and the cock red cells were washed with PBS, and the final concentration of 1% (V / V) was resuspended, 50 μl of 2 times series diluted plant agglutinum solution solution and the like The erythrocyte solution was mixed in a 96-well round bottom plate, and the flu antigen (B / Maryland / 15 / 2016, NIBSC-UK-EN63QG, NIBSC code: 18 / 104, HA: 69 μg / ml) was diluted with The positive control, the PBS solution was used as a negative control, and the plate was incubated under room temperat...

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Abstract

The present invention discloses a plant lectin succ-Con A for treating coronavirus infection and its application. The present invention finds for the first time that the plant lectin succ-Con A targets both SARS-CoV-2 and SARS-CoV-2 mutant strains, SARS‑CoV and MERS‑CoV coronavirus strains have broad-spectrum antiviral effects and can be used as broad-spectrum antiviral preparations or pharmaceutical active ingredients, providing new ideas for the prevention and treatment of coronavirus infections.

Description

Technical field [0001] The present invention belongs to the field of medical technology, and more particularly to plant aggregate SUCC-CON A, more particularly, to a plant lectin Succ-con A, which is used to treat coronavirus infection. Background technique [0002] New coronary virus (2019-NCOV) was officially classified by the International Virus Classification Committee on February 12, 2020, was named severned acute respiratory syndrome crosent virus 2 (Severe Acute Respiratory Syndromecoronavirus 2, SARS-COV-2), World Health Organization ( WHO) officially named COVID-19 in the same day. SARS-COV-2 belongs to the beta-crowed virus, a native-chain RNA virus whose nucleic acid sequence is 79.5% of SARS-COV, and the consistency of the bat coronavirus Bat Cov Ratg13 is as high as 96.2%, The beta coronavirus detected in the bat body is more similar, and SARS-COV-2 is the seventh member of the infected human coronaviral family. [0003] Both SARS-COV-2, SARS-COV and MERS-COV belong ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P31/14
CPCA61K38/168A61P31/14
Inventor 王佑春王兰黄维金王文波李倩倩吴佳静段茂芹武刚于传飞郭璐韵
Owner NAT INST FOR FOOD & DRUG CONTROL
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