Functional KASP molecular marker of wheat powdery mildew resistance gene PmV and application thereof

Abstract

The invention discloses a functional KASP molecular marker of a wheat powdery mildew resistance gene PmV. The KASP molecular marker is KASP-PmV, the marker is a nucleotide sequence obtained by amplifying a DNA template by a KASP-PmV primer designed according to the 3214th basic group of the gene PmV, and the nucleotide sequence of the KASP molecular marker is as shown in SEQ ID NO: 1; and the KASP-PmV primer comprises a haynaldia villosa allelic upstream primer KASP-PmV-F, a wheat allelic upstream primer KASP-PmV-H and a shared downstream primer KASP-PmV-C. The invention further discloses application of the functional KASP molecular marker of the wheat powdery mildew resistance gene PmV in identifying whether the gene PmV is carried in a wheat breeding line / variety or not and in performing molecular marker-assisted selective breeding by utilizing the gene PmV. The marker can specifically detect the gene PmV and realize high-throughput and platformization detection and tracking of the gene PmV under the background of common wheat, and also can accelerate breeding utilization of the gene PmV under the background of common wheat, so that the marker can be efficiently applied to wheat molecular marker-assisted selection breeding.

Description

technical field [0001] The invention relates to the fields of biotechnology and genetic breeding, in particular to a functional KASP molecular marker of wheat powdery mildew resistance gene PmV and its application. Background technique [0002] Common wheat (Triticum aestivum L., 2n=42, AABBDD) is one of the three major food crops in my country, and is very important to ensure national food security. In wheat production, the occurrence of diseases seriously affects the yield and quality of wheat. Among the main diseases of wheat, powdery mildew is one of the most serious diseases. It is caused by the infection of powdery mildew brucei. It usually affects the leaves. In severe cases, the spores can also infect the ears. After wheat is infected with powdery mildew, it can generally cause 10-15% of the yield, in severe cases, it can reduce the yield by 40%, and affect the quality of wheat to a certain extent (Singh et al. Disease impact on wheat yield potential and prospects o...

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Problems solved by technology

[0003] However, the powdery mildew resistance of the existing popularized varieties in my country is not optimistic. Among the main popularized varieties, the proportion of broad-spectrum resistant varieties is very low, less than 5%, and more powdery mildew resistance of breeding lines participating in national regional trials The same is not optimistic (Li Hongjie et al., Resistance response of Chinese wheat varieties to powdery mildew and detection of resistance genes. Acta Crops Sinica. 2011, 37:943-954; Tang et al. (2018) The potential role of powdery mildew-resistance genePm40 in Chinese wheat-breeding programs in the post-PmV Era.Engineering-Prc,2018,4:500-506)

However, gene PmV, as a powdery mildew resistance gene with important breeding value, has been cloned and has broad-spectrum resistance. The powdery mildew gene Pm21 is a homologous gene with high sequence similarity, which makes it difficult to develop specific KASP sequences. Therefore, the industry has not realized the development and utilization of KASP molecular markers for high-throughput detection of gene PmV, especially functional KASP markers. to report

This seriously affects the efficient utilization of PmV genes in wheat genetic improvement

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