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Application of baicalein in preparation of drugs for preventing and/or treating diseases caused by novel coronavirus infection

A technology of coronavirus and baicalein, which is applied in the field of medicine to achieve the effects of virus suppression and improvement of lung inflammation, improvement of respiratory function, and improvement of histopathological changes

Active Publication Date: 2021-08-13
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, HCMV-induced increases in the levels of NF-κB and cell cycle regulatory proteins associated with immediate early protein function were absent

Method used

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  • Application of baicalein in preparation of drugs for preventing and/or treating diseases caused by novel coronavirus infection
  • Application of baicalein in preparation of drugs for preventing and/or treating diseases caused by novel coronavirus infection
  • Application of baicalein in preparation of drugs for preventing and/or treating diseases caused by novel coronavirus infection

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Prediction of baicalein's anti-coronavirus activity at the molecular level by computer virtual docking

[0088] Using the high-resolution crystal structure of SARS-CoV-2 coronavirus key protein 3CL hydrolase (Mpro) (note: the coordinates of the crystal structure can be downloaded from the PDB protein structure database (Protein Data Bank, PDB) (PDB ID: 6LU7)), Discovery studio software was used for computer virtual docking, and the molecular docking results are shown in Table 1. figure 1 Shown is the interaction diagram of Mpro and baicalein.

[0089] Table 1 Docking results of baicalein and Mpro

[0090] Baicalein docking energy Conformation 1 34.8954 Conformation 2 34.4027 Conformation 3 31.7587 Conformation 4 31.6989 Conformation 5 31.4707 Conformation 6 31.3751 Conformation 7 30.9768 Conformation 8 30.9701 Conformation 9 30.9509 Conformation 10 30.8861

[0091] The results of computer virt...

Embodiment 2

[0093] Effect of baicalein on the infectivity of SARS-CoV-2 new coronavirus

[0094] [Test drug]: see Table 2 for drug name and concentration.

[0095] [Cells]: VeroE6 cells, preserved and provided by the Pathogen Center, Institute of Medical Experimental Animals, Chinese Academy of Medical Sciences.

[0096] [Virus]: New coronavirus (SARS-CoV-2), with a titer of 105TCID50 / ml, was stored at -80°C by the Pathogen Center of the Institute of Medical Experimental Animals, Chinese Academy of Medical Sciences. A virus titer of 100TCID50 was used.

[0097] 【experimental method】

[0098] (1) In a sterile 96-well culture plate, add 200 μl to each well with a concentration of 5×10 4 cell / ml Vero E6 cells, 5% CO at 37°C 2 Cultivate for 24 hours;

[0099] (2) Dilute the test drug to 2 concentrations, each concentration has 5 replicate wells, 100 μl per well, and then add an equal volume of 100 TCID to each well 50 Virus, act for 1h;

[0100] (4) After 1 hour, discard the cell cultu...

Embodiment 3

[0117] Study on the pharmacological effects of baicalein on pneumonia at the cellular level

[0118]Select inflammatory stimuli bacterial lipopolysaccharide (LPS) and baicalein, use in vitro cell models, use in vitro cell models to investigate the cytotoxicity of inflammatory stimuli and baicalein and the inflammation-related signals in mouse macrophage Raw264.7 pathways to confirm the anti-inflammatory activity of selected baicaleins. In addition, normal human lung bronchial epithelial cells BEAS-2b were used to observe the characteristics and selectivity of baicalein on the proliferation activity of normal lung cells.

[0119] 【Sample preparation】

[0120] Baicalein (Baicalein, BC) was provided by the Institute of Materia Medica, Chinese Academy of Medical Sciences. Its structure is shown in formula (I), with a purity >98%; Caspase-1 small molecule inhibitor VX-765 was purchased from Shanghai Hanxiang Biotechnology Co., Ltd. Purity > 98%; non-steroidal anti-inflammatory dr...

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Abstract

The invention discloses an application of baicalein in preparation of drugs for preventing and / or treating diseases caused by novel coronavirus infection. Specifically, the invention relates to the application of the baicalein and a pharmaceutical composition containing baicalein in preparation of drugs for preventing and / or treating diseases caused by the novel coronavirus (SARS-CoV-2) infection, and the infectious diseases comprise mild, medium and severe infections caused by novel coronavirus, especially novel coronavirus pneumonia.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to the application of baicalein in the preparation of drugs for preventing and / or treating novel coronavirus infection diseases. Specifically, it relates to the application of baicalein and pharmaceutical compositions containing baicalein in the prevention and / or treatment of new coronavirus (SARS-CoV-2, hereinafter also referred to as "new coronavirus") infectious diseases, including the Mild, moderate, and severe infections caused by viruses, including but not limited to new coronary pneumonia. Background technique [0002] Coronaviruses are a class of RNA viruses with envelopes, composed of a single ribonucleic acid RNA, and belong to single-stranded RNA viruses. At present, about 15 different coronavirus strains have been discovered, which can infect humans, mice, pigs, cats, dogs, and avian vertebrates. Coronaviruses (CoVs) belong to the Coronaviridae family and are divided in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K45/06A61P31/14A61P11/00
CPCA61K31/352A61K45/06A61P31/14A61P11/00A61K2300/00Y02A50/30
Inventor 杜冠华吕扬王珂刘江宁秦川宋俊科杨世颖杨德智张丽张莉王金华张雯袁天翊邢逞
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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