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Synthesis method of C-N@GC nano material and application of C-N@GC nano material in detection of cysteine

A technology of nanomaterials and synthesis methods, applied in the field of electrical analysis, can solve the problems of expensive equipment and long detection time, and achieve the effects of simple and convenient preparation methods, rapid detection and high sensitivity

Active Publication Date: 2021-08-10
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a synthesis method of C-N@GC nanomaterials and its detection application in cysteine, so as to solve the problems of long detection time and expensive equipment required in the above-mentioned background technology

Method used

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  • Synthesis method of C-N@GC nano material and application of C-N@GC nano material in detection of cysteine

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Experimental program
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Effect test

Embodiment 1

[0025] A method for synthesizing C-N@GC nanomaterials, comprising the following steps:

[0026] (1) Synthesis of ZIF-8: Pour 2-methylimidazole in methanol (6.16g, 150mL) into Zn(NO) 2 ·6H 2 O in methanol solution (5.95g, 150mL); secondly, the above-mentioned mixture was stirred at room temperature for 24h to obtain a milky white liquid; then the milky white liquid was washed 3 times with methanol and collected by centrifugation; Vacuum dried overnight to obtain ZIF-8; and 2-methylimidazole and Zn(NO) 2 ·6H 2 The mass ratio of O is 6.16:5.95;

[0027] (2) Synthesis of ZIF-8@ZIF-67: ZIF-8 (0.5 g) synthesized in step (1) was dispersed in methanol solution (100 mL) by ultrasonic waves, and after stirring for 10 min, the methanol of 2-methylimidazole solution (6.16g, 100mL) and Co(NO) 2 ·6H 2 O methanol solution (5.82g, 100mL) was poured into the above solution; secondly, the above mixed solution was stirred at room temperature for 24h to obtain a purple solution; then the pu...

Embodiment 2

[0030] The application of C-N@GC nanomaterials in cysteine ​​detection includes the following steps:

[0031] S1. Configuration of modifier solution: transfer 5 mg of C-N@GC synthesized in Example 1 into a mortar, grind it into powder, and put the ground C-N@GC into 1 ml of water-ethanol solution ( Volume ratio 3:1), the ground C-N@GC can be uniformly dispersed in 1 ml of water-ethanol solution (volume ratio 3:1), and 20 μL naphthol solution (5wt%) was added dropwise thereto, Obtain a uniform modifier solution by sonicating for 2 hours;

[0032] S2. Modification on the surface of the glassy carbon electrode: take 3 μL of the modifier solution in S1 and evenly drop it on the surface of the polished glassy carbon electrode, and dry it at room temperature to finally obtain a C-N@GC modified glassy carbon electrode;

[0033] S3. Using the C-N@GC modified glassy carbon electrode obtained in S2 for the electrochemical detection of cysteine.

[0034] The specific application proces...

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Abstract

The invention discloses a synthesis method of a C-N-GC nano material and application of the C-N@GC nano material in detection of cysteine. The synthesis method comprises the following steps of: stirring and mixing a methanol solution of 2-methylimidazole and a methanol solution of Zn (NO) 2.6 H2O at room temperature to obtain a precipitate ZIF-8, and centrifuging, washing and drying the precipitate to obtain a white powder product; dispersing the synthesized ZIF-8 in a methanol solution by using ultrasonic waves, stirring for 10 minutes, and pouring the methanol solution of 2-methylimidazole and the methanol solution of Co (NO) 2.6 H2O into the solution; stirring the mixed solution at room temperature to obtain a precipitate ZIF-8@ZIF-67, and centrifuging, washing and drying the precipitate to obtain a purple powder product; and taking and putting a certain amount of ZIF-8@ZIF-67 into a muffle furnace, heating at a heating rate of 2 DEG C per minute in an Ar atmosphere, calcining at a constant temperature of 920 DEG C for 2 hours, and cooling to room temperature to obtain a black solid C-N@GC. The C-N@GC is modified on the surface of a glassy carbon electrode for cysteine detection. The application of the nano modified electrode in cysteine detection is promoted.

Description

technical field [0001] The present invention relates to the technical field of electrical analysis, and in particular relates to a synthesis method of C-N@GC nanomaterials and its detection application in cysteine. Background technique [0002] Cysteine ​​(Cys) is a small molecule containing a sulfhydryl group. Due to its chemical instability, cysteine ​​plays a variety of roles in physiological and biological processes. In biological processes, cysteine ​​is a precursor for the synthesis of glutathione, acetyl-CoA, cystine, etc. It can undergo reversible redox reactions under physiological conditions, which is essential for maintaining the tertiary and quaternary structures of proteins. In addition, cysteine ​​can also be used as a substrate to be catalyzed and metabolized into bioactive molecules such as pyruvate, sulfenic acid, and hydrogen sulfide, and further play multiple roles in the organism. Cysteine ​​deficiency in humans can lead to hair loss, slow growth, liver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 王志娟蔡永亮陈剑刘霞胡松
Owner NANJING UNIV OF TECH
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