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Bacillus megaterium strain LXB4070 and application thereof

A technology of Bacillus megaterium and strain, applied in the field of microorganisms, can solve the problem of no biocontrol effect, etc., and achieve the effects of improving biocontrol function, efficient inhibition ability, efficient growth promotion and biocontrol effect

Inactive Publication Date: 2021-08-06
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the single bacterial agents currently used in the agricultural field only have the effect of promoting growth or biocontrol. If no functional substances are added from exogenous sources, there are few bacterial agents that can achieve both.
Bacillus megaterium, as a typical microorganism that decomposes phosphorus, promotes potassium and silicon, can obviously promote the growth of crops; however, the existing Bacillus megaterium is mainly a growth-promoting bacterium, basically does not have Biocontrol
[0004] In order to solve the utilization status quo faced by existing bacillus megaterium (Bacillus megaterium), the present invention is specially proposed

Method used

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  • Bacillus megaterium strain LXB4070 and application thereof
  • Bacillus megaterium strain LXB4070 and application thereof
  • Bacillus megaterium strain LXB4070 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The present embodiment provides the preparation method of Bacillus megaterium (Bacillus megaterium) bacterial strain LXB4070, and the steps are as follows:

[0061] 1. Bacterial activation and culture

[0062] Bacillus megaterium (Bacillus megaterium) strain LXB4070 was streak-cultured on a solid medium from a frozen glycerol tube, and cultured at 36°C for 32 hours to obtain original slant seeds.

[0063] The above-mentioned original slant seeds were inoculated in a 250 mL Erlenmeyer flask, and cultured for 24 hours under the conditions of 180 r / min and 36° C. to obtain seed liquid.

[0064] 2. Liquid fermentation

[0065] The composition of the fermentation medium is: corn flour 35g / L, soybean meal flour 32g / L, peptone 1.5g / L, yeast powder 2.0g / L, potassium dihydrogen phosphate 2.0g / L, dipotassium hydrogen phosphate 2.0g / L, Ferrous chloride 0.05g / L, calcium carbonate 0.5g / L, calcium chloride 0.5g / L, magnesium sulfate 0.4g / L and manganese sulfate 0.1g / L.

[0066] The...

Embodiment 2

[0070] The present embodiment provides the preparation method of Bacillus megaterium (Bacillus megaterium) bacterial strain LXB4070, and the steps are as follows:

[0071] 1. Bacteria activation culture:

[0072] Bacillus megaterium (Bacillus megaterium) strain LXB4070 was streak-cultured on a solid medium from a frozen glycerol tube, and cultured at 36°C for 32 hours to obtain original slant seeds.

[0073] The above-mentioned original slant seeds were inoculated in a 250 mL Erlenmeyer flask, and cultured for 24 hours under the conditions of 180 r / min and 36° C. to obtain seed liquid.

[0074] 2. Liquid fermentation

[0075] The composition of the fermentation medium is: corn flour 35g / L, soybean meal flour 32g / L, peptone 1.5g / L, yeast powder 2.0g / L, potassium dihydrogen phosphate 2.0g / L, dipotassium hydrogen phosphate 2.0g / L, Ferrous chloride 0.05g / L, calcium carbonate 0.5g / L, calcium chloride 0.5g / L, magnesium sulfate 0.4g / L and manganese sulfate 0.1g / L.

[0076] The fil...

Embodiment 3

[0080] This embodiment provides a method for preparing a biocontrol and growth-promoting microbial agent containing Bacillus megaterium (Bacillus megaterium) strain LXB4070, the steps are as follows:

[0081] a. Liquid fermentation of Bacillus megaterium (Bacillus megaterium) strain LXB4070

[0082] a1. Bacteria activation culture

[0083] Bacillus megaterium (Bacillus megaterium) strain LXB4070 was streak-cultured on a solid medium from a frozen glycerol tube, and cultured at 36°C for 32 hours to obtain the original slant seeds; then the original slant seeds were inoculated into a 250mL Erlenmeyer flask, Cultivate for 24 hours at 180r / min and 36°C.

[0084] a2. Fermentation culture

[0085] The composition of the fermentation medium is: corn flour 35g / L, soybean meal flour 32g / L, peptone 1.5g / L, yeast powder 2.0g / L, potassium dihydrogen phosphate 2.0g / L, dipotassium hydrogen phosphate 2.0g / L, Ferrous chloride 0.05g / L, calcium carbonate 0.5g / L, calcium chloride 0.5g / L, magnes...

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Abstract

The invention provides a Bacillus megaterium strain LXB4070 and application thereof. The preservation number of the Bacillus megaterium strain LXB4070 is CGMCC NO. 21867. The Bacillus megaterium strain LXB4070 has remarkable growth-promoting characteristics of phosphate solubilizing, potassium promoting, silicon solubilizing and the like, and basically does not have a biocontrol characteristic. A biocontrol growth-promoting microbial agent comprises the Bacillus megaterium strain LXB4070 and / or a fermentation product thereof, not only retains the original remarkable growth-promoting characteristics of phosphate solubilizing, potassium promoting, silicon solubilizing and the like, but also has efficient biocontrol characteristic, has efficient inhibiting ability on fusarium oxysporum, ginger stem basal rot pathogenic bacteria, botrytis cinerea, apple fusarium pathogenic bacteria and potato black scurf pathogenic bacteria, and has obvious growth-promoting effect on green Chinese onion, apples, grapes, strawberries, potatoes and the like and obvious gain effect on fruits.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Bacillus megaterium strain LXB4070 and an application thereof. Background technique [0002] Microbial fertilizer is a biological fertilizer produced by industrialized cultivation and fermentation of one or several beneficial microorganisms. Microbial fertilizers are usually divided into two categories: one is to increase the supply of plant nutrient elements through the life activities of the microorganisms contained in it, resulting in the improvement of plant nutrition, thereby increasing production. The representative variety is bacterial fertilizer; the other is Microbial fertilizers in a broad sense, although they also increase crop production through the microbial life activities contained in them, are not limited to improving the supply level of plant nutrients, but also include secondary metabolites produced by them, such as hormones. stimulating effect. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01N63/27A01P3/00A01P21/00C12R1/11C12R1/38
CPCC12N1/20A01N63/22A01N63/27
Inventor 周立华李丽艳杨娜孙杉杉李婧葛振宇王东杜迎辉刘洪钧徐志文
Owner 领先生物农业股份有限公司
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