Method for rapidly screening illegally added bisacodyl in Chinese patent medicines and health foods

Abstract

The invention provides a method for rapidly screening illegally added bisacodyl in Chinese patent medicines and health foods, wherein the method comprises the following steps: (1) dripping a first test solution and a first reference solution on a silica gel thin-layer plate, placing under an ultraviolet lamp, inspecting to detect corresponding main spots, and screening through a liquid chromatograph; (2) injecting the second test solution and the second reference substance solution into the liquid chromatograph, recording a chromatogram and a DAD spectrogram, and if a chromatographic peak consistent with the reference substance in chromatographic retention time and DAD spectrum is detected, performing confirmation by adopting a liquid chromatograph-mass spectrometer; and (3) taking the second test solution, the second reference solution and the second negative sample solution, injecting into the liquid chromatograph-mass spectrometer, detecting corresponding chromatographic peaks, and determining illegally added bisacodyl. The method disclosed by the invention is high in sensitivity, good in selectivity, strong in confirmation and simple, convenient and rapid to operate, and can be used for qualitative screening and accurate confirmation of illegally added bisacodine in the Chinese patent medicines and health-care foods.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a method for quickly screening illegally added bisacodyl in Chinese patent medicines and health food. Background technique [0002] At present, bisacodyl chemicals are illegally added in weight-loss, weight-loss Chinese patent medicines, weight-loss health foods and weight-loss products. They are mainly used to treat constipation, clean the intestines before and after surgery, etc., so they have weight-loss effects, but non-standard use may Cause abdominal cramps, excessive diarrhea, proctitis, etc., and cause damage to people's health. At present, there is a lack of fast and accurate detection methods for this substance, and the main detection methods are still non-aqueous titration, liquid chromatography, etc. The detection speed is slow, the accuracy is low, and it is prone to "false positive" and "false negative". Contents of the invention [0003] The purpose of th...

AI Technical Summary

Problems solved by technology

[0002] At present, bisacodyl chemicals are illegally added in weight-loss, weight-loss Chinese patent medicines, weight-loss health foods and weight-loss products. They are mainly used to treat constipation, clean the intestines before and after surgery, etc., so they have weight-loss effects, but non-standard use may Cause abdominal cramps, excessive diarrhea, proctitis, etc., and cause damage to people's health

At present, there is a lack of fast and accurate detection methods for this substance, and the main detection methods still remain in non-aqueous titration, liquid chromatography, etc., with slow detection speed and low accuracy, and are prone to "false positives" and "false negatives".

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