A kind of magnesium nitrogen doped carbon point and its preparation method and the application in improving plant photosynthesis

A photosynthesis and carbon dot technology, applied in the field of nanomaterial biological effect research, can solve the problems of low photosynthesis efficiency and limited light capture ability of plants, photosynthetic system activity, etc., to promote synthesis and metabolism, improve photosynthetic activity, and accelerate growth. Effect

Active Publication Date: 2022-06-07
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the photosynthetic efficiency of plants is much lower than its theoretical value, and the main limiting factors include: (1) the limited light harvesting ability of plants themselves and (2) limited photosynthetic system activity

Method used

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  • A kind of magnesium nitrogen doped carbon point and its preparation method and the application in improving plant photosynthesis
  • A kind of magnesium nitrogen doped carbon point and its preparation method and the application in improving plant photosynthesis
  • A kind of magnesium nitrogen doped carbon point and its preparation method and the application in improving plant photosynthesis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Preparation of magnesium-nitrogen-doped carbon dots (Mg,N-CDs): Mg,N-CDs were prepared by a one-step hydrothermal method using citric acid, ethanolamine and magnesium hydroxide as raw materials. Citric acid, ethanolamine and magnesium hydroxide were dissolved in ultrapure water and sonicated for 30 min. Magnesium hydroxide, which is slightly soluble in water, can be completely dissolved due to the chelation of citric acid. Then, the mixed solution obtained by ultrasonic treatment was poured into the lining of a 50 mL polytetrafluoroethylene reactor, put into the reactor, and reacted at 200° C. for 6 hours. After the reaction kettle was lowered to room temperature, the brown product was filtered with a filter head with a pore size of 0.22 μm, and then dialyzed with a dialysis bag (MW 100-500) for 12 hours, and the dialysate was ultrapure water. After freeze-drying, magnesium-nitrogen-doped carbon dots (Mg,N-CDs) can be obtained.

[0031] In order to study the existing f...

Embodiment 2

[0033] The magnesium-nitrogen-doped carbon dots (Mg, N-CDs) obtained in Example 1 were characterized:

[0034] Characterization method: The morphology of Mg,N-CDs was observed by JEM-2100F field emission transmission electron microscope (TEM) and SPA-400 atomic force microscope (AFM). Characterization was performed using a Thermo Scientific Nicolet 6700FTIR spectrometer and Thermo Scientific K-Alpha X-ray Photoelectron Spectroscopy (XPS). The fluorescence emission spectra and fluorescence lifetime decay curves of Mg,N-CDs were measured by F-7000Hitachi and FLS1000-stm fluorescence spectrometers, respectively. A PerkinElmer UV-Vis spectrophotometer (Lambda 750) was used to determine the UV-Vis light absorption curves of Mg,N-CDs. At the same time, we also used TGA-DSC thermogravimetric (Labsys STA) combined with mass spectrometry (LC-DZOOM Pro) (TG-MS) system to determine the thermal decomposition curve and product composition of Mg,N-CDs from room temperature to 800℃. Finall...

Embodiment 3

[0042] The effect of magnesium-nitrogen-doped carbon dots (Mg, N-CDs) obtained in Example 1 on the growth of rice plants:

[0043] The treatment method of magnesium-nitrogen-doped carbon dots on rice plants: First, use 75% ethanol solution to sterilize rice seeds (Huahang No. 31), thoroughly wash them with deionized water, spread them evenly in the germination tray, and add 50% concentration of nutrients solution and incubated at 30°C in the dark. After 3 days, the cover of the germination tray was lifted, the seeds were basically completely germinated, and placed in a greenhouse (22-30°C, 70% relative humidity, 400 μmol m -2 ·s -1 The light intensity, the photoperiod is 16h light / 8h dark) continued to cultivate to the 7th day. The rice seedlings with uniform growth state were selected and transplanted into black square plastic boxes, 12 plants per box, and the nutrient solution in the box was replaced twice a week. Different concentrations of Mg, N-CDs (50, 100 and 300 μg·...

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Abstract

The invention belongs to the field of biological effect research of nanometer materials, and discloses a magnesium-nitrogen-doped carbon point, a preparation method thereof and an application in improving plant photosynthesis. The preparation method comprises the steps of: dissolving citric acid, ethanolamine and magnesium hydroxide in ultrapure water, ultrasonically treating them, pouring them into a reaction kettle, and reacting at 200°C for 6 hours; Filter through a filter head of μm, and then dialyze for 12 hours; freeze-dry to obtain magnesium nitrogen doped carbon dots. After recombining with chloroplasts, its excitation energy will be absorbed by chloroplasts for photosynthesis, increasing photosynthetic activity. After foliar spraying is applied to rice plants, Mg, N‑CDs can be evenly distributed in the leaf cells, regulate the expression of related enzyme genes, promote the synthesis and metabolism of chlorophyll in the leaves, and maintain the activity of chlorophyll molecules at a high level , thereby increasing the photosynthetic activity of leaves. Finally, plant growth is significantly enhanced.

Description

technical field [0001] The invention belongs to the field of nanomaterial biological effect research, and in particular relates to a magnesium-nitrogen-doped carbon dot, a preparation method thereof, and use in improving plant photosynthesis. Background technique [0002] Photosynthesis is an important physiological process in plant growth and is of great significance to food yield. Improving crop yield by regulating photosynthesis has become a hot topic of concern for many researchers. The current plant photosynthesis efficiency is much lower than its theoretical value, and the main limiting factors include: (1) the limited ability of the plant itself to capture light and (2) the limited activity of the photosynthetic system. As light harvesters, photosynthetic pigments are involved in almost all physiological processes of plant photosynthesis, including light harvesting, energy transfer, and energy conversion. Magnesium and nitrogen are important components of plant phot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15A01G7/06
CPCC09K11/65C09K11/0883B82Y20/00B82Y40/00C01B32/15A01G7/06
Inventor 刘应亮李亚东胡超凡庄健乐雷炳富张学杰许晓凯郑明涛李唯张浩然
Owner SOUTH CHINA AGRI UNIV
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