Stirring Control Method Based on PCR Amplification Chamber

Abstract

The invention relates to a stirring control method based on a PCR amplification chamber, the amplification chamber contains reactants to be amplified, freeze-dried balls and memory metal; when the reactants to be amplified and freeze-dried balls enter the amplification chamber When within, record the first length and the second length of the memory metal at the first temperature and the second temperature; establish the first disturbance function T1 (t1i, L1i, Q1i) and the second disturbance function T2 (t2i, L2i, Q2i). By setting the memory metal in the amplification chamber, multiple reaction cycles are set in the actual amplification reaction process to make the amplification reaction more sufficient, and the deformation generated by the memory metal at the deformation temperature can be used in the amplification chamber. Disturbance of the reactant to be amplified and the freeze-dried sphere makes the related chemical reaction more uniform and more complete under the action of the disturbance.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a temperature control method based on a PCR amplification chamber. Background technique [0002] In the gene chip detection process, temperature control is the decisive factor in the PCR (Polymerase Chain Reaction, polymerase chain reaction) amplification process, and it is the key technology that determines whether the fluorescent signal can be seen. [0003] Temperature control usually includes three stages of temperature control, which are high temperature (92°C-96°C), medium temperature (69°C-73°C) and low temperature (52°C-56°C). In a complete PCR amplification process, the gene chip will be denatured in the high temperature stage, then annealed in the low temperature stage, and finally extended in the middle temperature stage. Completing the above three stages completes a PCR amplification. Generally, multiple above-mentioned processes are required to reach the predet...

AI Technical Summary

Problems solved by technology

However, the existing temperature monitoring methods are not very sensitive to temperature control and adjustment, which will have a certain impact on the PCR reaction, thus affecting the final fluorescence detection results.

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