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Extraction method of aronia melanocarpa leaf oligosaccharide and application of aronia melanocarpa leaf oligosaccharide in preparation of antibacterial preservative film

The invention relates to a technology of Rowan nigra and an extraction method, which are applied in the directions of fruit and vegetable preservation, application, food ingredients containing natural extracts, etc., and can solve the problem that polysaccharides cannot be effectively utilized, cannot be directly applied to industrial production, and the proportion of high-molecular-weight polysaccharides is high. High problems, to achieve the effect of good poverty alleviation and income increase, prolonging the shelf life of food, and simple application methods

Active Publication Date: 2021-07-27
周雪艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Aronia black fruit mainly focuses on the purification and antioxidant application of polyphenols, flavonoids, anthocyanins and other substances in fruit and pomace, and the leaves of Aronia black fruit are important in the forestry industry. Most of the main wastes are naturally fertilized in the land, resulting in a waste of resources
[0003] At present, there are few ways to develop and utilize the leaves of Aronia nigritica, and the polysaccharides in the leaves cannot be effectively utilized, and the existing method for extracting polysaccharides from Aronia nigrutica leaves has a high proportion of large molecular weight polysaccharides, and the molecular weight The difference is large and the properties are not uniform, so it cannot be directly applied to industrial production
The existing plastic wrap is mainly to improve the plastic composition or molecular structure of the plastic wrap, and the antibacterial effect is general

Method used

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  • Extraction method of aronia melanocarpa leaf oligosaccharide and application of aronia melanocarpa leaf oligosaccharide in preparation of antibacterial preservative film
  • Extraction method of aronia melanocarpa leaf oligosaccharide and application of aronia melanocarpa leaf oligosaccharide in preparation of antibacterial preservative film
  • Extraction method of aronia melanocarpa leaf oligosaccharide and application of aronia melanocarpa leaf oligosaccharide in preparation of antibacterial preservative film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 A method for extracting oligosaccharides from Aronia nigra leaf oligosaccharides

[0036] This embodiment includes the following steps carried out in sequence:

[0037] Get Aronia adenocarpus leaves, wash, dry, pulverize, and sieve through a 60-mesh sieve to obtain Arana arborica leaf powder, which is stored in a desiccator for subsequent use;

[0038] According to the weight ratio of 2:1:2, take pectinase powder, acid protease powder and cellulase powder and stir and mix evenly to prepare combined enzyme powder, which is set aside.

[0039] S1. Preparation of Aronia nigra leaf oligosaccharide solution:

[0040] Weigh 1kg of Aronia arvensis leaf powder, add 10L of water, add 2g of combined enzyme powder, stir and mix, at room temperature, microwave treatment at 500W for 15min, filter and discard the filter residue, and obtain Aronia arborica arvensis leaf oligosaccharide solution.

[0041] Wherein, the weight-to-volume ratio of Aronia oleifera leaf powder a...

Embodiment 2-6

[0046] Example 2-6 Method for extracting oligosaccharides from Aronia nigra leaf oligosaccharides

[0047] Examples 2-6 are respectively a method for extracting oligosaccharides from Aronia nigra leaf oligosaccharides, and their steps are basically the same as those in Example 1, the only difference being that the process parameters are different, see Table 1 for details:

[0048] Table 1 embodiment 2-6 process parameters

[0049]

[0050]

[0051] The Aronia oligosaccharides T2-T6 prepared in Examples 2-6 can be respectively applied to the preparation of antibacterial fresh-keeping film.

[0052] Verification test:

[0053] The weight-average molecular weight, chemical composition and antibacterial effect of T1-T6 oligosaccharides from the leaves of Aronia nigra were detected through specific experiments.

[0054](1) adopt HPLC method to detect the weight-average molecular weight of Sorbus arvensis leaf oligosaccharide T1-T6, concrete test condition is: chromatographi...

Embodiment 7-12

[0069] Example 7-12 The application of antibacterial preservative film prepared by oligosaccharides from Aronia nigra leaf oligosaccharides

[0070] Examples 7-12 are respectively an application of preparing antibacterial preservative film for Aronia oleifera leaf oligosaccharides, which are used to prepare antibacterial preservative films for Aronia oleifera leaf oligosaccharides. The preparation steps are:

[0071] Take Aronia adenocarpus leaf oligosaccharides and water with a weight ratio of 1:1-10 and mix them with water, add hot-melted biodegradable polymer materials, and make the weight of Aronia adenocarpus leaf oligosaccharides equal to or equal to that of biological 1-10% of the weight of the degradable polymer material is stirred and mixed, and the film is coated by a film coating machine to prepare the antibacterial and fresh-keeping film of Aronia oleifera leaf oligosaccharides, which are marked as M1-M6 in sequence;

[0072] Among them, the biodegradable polymer m...

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Abstract

The invention belongs to the technical field of plant leaf oligosaccharide extraction and application, and discloses an extraction method of aronia melanocarpa leaf oligosaccharide and application of the aronia melanocarpa leaf oligosaccharide in preparation of a bacteriostatic preservative film. Pectinase powder, acid protease powder and cellulase powder are reasonably proportioned, and oligosaccharide with molecular weight of 8000 Da or less in aronia melanocarpa leaves is extracted by microwaves. The extraction method is fast and environment-friendly, and the molecular weights of the obtained oligosaccharides are similar. The extracted aronia melanocarpa leaf oligosaccharide is mixed with a biodegradable high polymer material, the mixture is applied to preparation of the degradable antibacterial preservative film, waste utilization of the aronia melanocarpa leaves is achieved, and the prepared preservative film can inhibit growth of microorganisms and prolong the shelf life of food.

Description

technical field [0001] The invention belongs to the technical field of plant leaf oligosaccharide extraction and application, and relates to the extraction of oligosaccharides in leaves and the application of preparing antibacterial and fresh-keeping films, in particular to a method for extracting oligosaccharides from leaves of Sorbus arvensis and a method for preparing antibacterial and fresh-keeping films application. Background technique [0002] Sorbus nigra is a perennial deciduous shrub belonging to the family Rosaceae. It is an afforestation species introduced in arid areas in my country. It is widely cultivated in Northeast China, with a planting area of ​​more than 1,000 hectares. Aronia nigra is rich in flavonoids, terpenes, phenols, polysaccharides and anthocyanins. At present, the research on Aronia black fruit mainly focuses on the purification and anti-oxidation application of polyphenols, flavonoids and anthocyanins in fruits and pomace. Most of the main wa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3562A23B7/154B65D65/02B65D65/38B65D81/24B65D85/34C08B37/00
CPCA23L3/3562A23B7/154C08B37/006C08B37/0003B65D65/02B65D65/38B65D81/24B65D85/34A23V2002/00A23V2200/10A23V2250/21Y02A40/22Y02P60/40
Inventor 周雪艳朱娜刘云
Owner 周雪艳
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