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Bee venom-purifying method comprising viral clearance process and composition for preventing or treating inflammatory disease by using same

A purification method, a technology of bovine viral diarrhea, which is applied to the preparation method of peptides, drug combinations, chemical instruments and methods, etc., and can solve problems such as the influence of the content of physiologically active substances

Pending Publication Date: 2021-07-23
UBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, if the above-mentioned virus removal process is simply applied to the method of purifying bee venom, there will be a problem of affecting the content of the main physiologically active substances present in the bee venom, so studies to minimize such problems are required

Method used

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  • Bee venom-purifying method comprising viral clearance process and composition for preventing or treating inflammatory disease by using same
  • Bee venom-purifying method comprising viral clearance process and composition for preventing or treating inflammatory disease by using same
  • Bee venom-purifying method comprising viral clearance process and composition for preventing or treating inflammatory disease by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078]Example 1: Acid pH 2 Treatment

[0079] Bee venom was prepared by adding bee venom to purified water and completely dissolving the bee venom to 5.0% by weight. First, the bee venom was filtered through a sterilizing filter with 0.22 μm pores (pores) under reduced pressure filtration. Afterwards, use 0.2M concentration of hydrochloric acid to treat for 1 hour to 15 hours to adjust the pH value of the bee venom to 2, thus after inactivating the virus for the first time, use 0.2M concentration of sodium hydroxide to treat the pH value of the bee venom The pH is corrected to 5. Then, under the condition of reduced pressure filtration, the bee venom is pre-filtered through a membrane filter with pores of 0.22 μm, then rapidly frozen at a temperature below -80°C, and freeze-dried in a vacuum state below 0.5 torr to prepare a powder. bee venom. In this case, a control group (Control) was prepared by freeze-drying without acid treatment.

Embodiment 2

[0080] Example 2: Acid pH 3 Treatment

[0081] After adjusting the pH value of the bee venom to 3 with hydrochloric acid at a concentration of 0.2M, it was treated for 1 hour to 15 hours. Bee venom was prepared in the same manner as in Example 1 except that the pH value of the bee venom was adjusted to 2.

Embodiment 3

[0082] Example 3: Acid pH 4 Treatment

[0083] After adjusting the pH value of the bee venom to 4 with hydrochloric acid at a concentration of 0.2M, it was treated for 1 hour to 15 hours. Bee venom was prepared in the same manner as in Example 1 except that the pH of the bee venom was adjusted to 4.

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Abstract

The present invention relates to a bee venom-purifying method comprising a virus clearance process and a composition for preventing or treating inflammatory disease by using same, the method comprising the steps: (a) preparing a bee venom solution containing bee venom; (b) adjusting the pH of the bee venom solution prepared in step (a) into 2.0 to 4.0 by acid treatment to primarily deactivate viruses; and (c) filtering the pH-adjusted bee venom solution of step (b) through a nanofilter of 10-20 nm to secondarily remove viruses.

Description

technical field [0001] The present invention relates to a method for purifying bee venom including a virus removal process and a composition for preventing or treating inflammatory diseases using the same. Background technique [0002] Bee venom is known to have strong antibacterial and anti-inflammatory effects, and has been used in folk and Chinese medicine formulations for arthritis, gout and other diseases. The main component of such bee venom is melittine, which is a peptide component known to have anti-inflammatory and antibacterial effects, strong analgesic effects, and immunity-enhancing effects. In order to use such bee venom in pharmaceuticals , In the purification process of bee venom, the virus must be removed. [0003] The reason why the validation of the virus removal process is required for pharmaceutical products of animal origin is to verify through experiments whether the virus removal process is designed in such a way as to effectively remove or inactivat...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/14C07K1/34C07K1/36A61K8/64A61K38/17A61P29/00A61Q19/00
CPCC07K14/43572C07K1/14C07K1/34C07K1/36A61K8/64A61K38/17A61P29/00A61Q19/00
Inventor 金健男裴根源黄智宣尹宣明
Owner UBIO
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