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Nutrient solution for improving transplanting survival rate of kok-saghyz tissue culture seedlings and improving method

A technique for transplanting survival rate and tissue culture seedlings, applied in the field of nutrient solution, can solve the problems of low transplanting survival rate, loss of precious materials, unguaranteed transplanting survival rate, etc. Plant growth, low price effect

Inactive Publication Date: 2021-07-23
北京玲珑蒲公英科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the tissue culture propagation efficiency of rubber grass is high at present, the transplanting survival rate cannot be guaranteed, especially in the transgenic research of rubber grass, the transplanting survival rate of regenerated plants is low, resulting in the loss of precious materials from time to time

Method used

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  • Nutrient solution for improving transplanting survival rate of kok-saghyz tissue culture seedlings and improving method
  • Nutrient solution for improving transplanting survival rate of kok-saghyz tissue culture seedlings and improving method
  • Nutrient solution for improving transplanting survival rate of kok-saghyz tissue culture seedlings and improving method

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Experimental program
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Embodiment 1

[0020] A method for improving the transplanting survival rate of rubber grass tissue culture seedlings, the steps comprising:

[0021] (1) Preparation of hydroponic nutrient solution storage solution. The content of the hydroponic nutrient solution storage solution can be as shown in Table 1 below. First, the macroelements, trace elements and iron salts are prepared to 40 times, 200 times and 200 times respectively The storage solution, mg / L means the number of milligrams of each component contained in each liter of the storage solution, and a certain amount of the storage solution is measured and diluted into a working solution when used.

[0022] Table 1 Hydroponic nutrient solution storage solution content

[0023]

[0024]

[0025] (2) To prepare hydroponic nutrient solution, take a certain amount of storage solution and dilute it into a 0.5-fold working solution, that is, measure 12.5mL of macroelement storage solution, 2.5mL of trace element storage solution and 2....

Embodiment 2

[0031] A method for improving the transplanting survival rate of rubber grass tissue culture seedlings, the steps comprising:

[0032] (1) Prepare hydroponic nutrient solution, prepare hydroponic nutrient solution storage solution according to the above Table 1, measure 25mL macroelement storage solution, 5mL trace element storage solution and 5mL iron salt storage solution according to 1000ml working solution when using, mix and distilled water Capacity up to 1000ml. The ingredients and content of the hydroponic nutrient solution (mg / L, the number of milligrams of each component in each liter of nutrient solution): ammonium nitrate 825, potassium nitrate 950, calcium chloride dihydrate 220, magnesium sulfate heptahydrate 185, Potassium dihydrogen phosphate 85, potassium iodide 0.415, boric acid 3.1, manganese sulfate tetrahydrate 11.15, zinc sulfate heptahydrate 4.3, sodium molybdate dihydrate 0.125, copper sulfate pentahydrate 0.0125, cobalt chloride hexahydrate 0.0125, ferr...

Embodiment 3

[0037]A method for improving the transplanting survival rate of rubber grass tissue culture seedlings, the steps comprising:

[0038] (1) Prepare hydroponic nutrient solution, prepare hydroponic nutrient solution storage solution according to the above Table 1, and use 1000ml working solution to measure 18.75mL macroelement storage solution, 3.75mL trace element storage solution and 3.75mL iron salt storage solution and mix And dilute to 1000ml with distilled water. The ingredients and content of the hydroponic nutrient solution (mg / L, the number of milligrams of each component in each liter of nutrient solution): ammonium nitrate 618.75, potassium nitrate 712.5, calcium chloride dihydrate 165, magnesium sulfate heptahydrate 138.75, Potassium dihydrogen phosphate 63.75, potassium iodide 0.3113, boric acid 2.325, manganese sulfate tetrahydrate 8.3625, zinc sulfate heptahydrate 3.225, sodium molybdate dihydrate 0.0938, copper sulfate pentahydrate 0.0094, cobalt chloride hexahydr...

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Abstract

The invention relates to a nutrient solution for improving the transplanting survival rate of kok-saghyz tissue culture seedlings. The nutrient solution comprises the following components: 412.5 to 825 parts of ammonium nitrate, 475 to 950 parts of potassium nitrate, 110 to 220 parts of calcium chloride dihydrate, 92.5 to 185 parts of magnesium sulfate heptahydrate, 42.5 to 85 parts of potassium dihydrogen phosphate and the like. The prepared nutrient solution is suitable for rooting and growth of kok-saghyz tissue culture seedlings, a seedling hardening device saves space, is low in price, economical and practical, and after transplanting and seedling hardening culture are conducted for 15-30 days through the steps, the final transplanting survival rate reaches 99% or above, and the plant biomass is increased by 50% or above.

Description

technical field [0001] The invention relates to a nutrient solution for improving the transplanting survival rate of rubber grass tissue culture seedlings and an improving method thereof. Background technique [0002] Natural rubber is an important strategic material and industrial raw material, mainly produced from Hevea brasiliensis. my country is a big consumer of natural rubber in the world, and more than 80% of it needs to rely on imports every year. Rubber grass (Taraxacum kok-saghyz), also known as Russian dandelion (Rubber Dandelion), is a perennial herb of the genus Dandelion in the Compositae family. It is native to Kazakhstan and Xinjiang, China, and other places. Its roots can synthesize high-quality natural rubber, and its rubber content accounts for The dry weight of the root is about 2.89%-27.89%, and the structure and performance are similar to the natural rubber produced by the Brasilian tree. Moreover, rubber grass has the advantages of strong adaptabilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G5/20C05G1/00A01G22/00A01G24/12A01G24/25A01G24/28
CPCC05B7/00C05G5/20A01G22/00A01G24/28A01G24/25A01G24/12C05C1/00C05C5/02C05D1/00C05D3/00C05D5/00C05D9/00C05D9/02
Inventor 王锋覃碧张立群刘实忠聂秋海杨玉双甘霖王肖肖
Owner 北京玲珑蒲公英科技发展有限公司
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