Construction method of novel brain metastatic tumor animal model

Abstract

The invention relates to the technical field of animal model construction, and discloses a construction method of a novel brain metastatic tumor animal model. The construction method disclosed by the invention comprises the following steps: separating a left common carotid artery and a left common carotid artery branch of the neck of a mouse; rotating the head of the mouse, lifting up a common carotid artery traction silk suture, and then temporarily blocking the common carotid artery; lifting up the telecentric end of the external carotid artery, and leading tumor cell suspension into an internal carotid artery towards the proximal end; and after completing leading in the tumor cell suspension, closing an external carotid artery, and opening the common carotid artery. According to the construction method, the pathophysiological process of human tumor brain metastasis is well simulated, the bleeding amount and the probability of tumor cell leakage in the modeling operation are reduced, the modeling success rate is increased, and the death rate of a model is reduced; and the prepared brain metastasis tumor animal model avoids metastasis of multiple organs, the whole-body tumor load of the mouse is reduced, and the mouse model prepared by the construction method can be used for researching the metastasis mechanism and tumor microenvironment of the brain metastasis tumor.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing a novel animal model of brain metastases. Background technique [0002] Currently, the animal model of brain metastases is constructed by injecting the tumor cell suspension into the arterial blood circulation of the mouse through a specific route, so that the tumor cells are delivered to the capillaries in the brain tissue through the blood flow, and finally the tumor cells penetrate the blood. BBB colonization in the brain parenchyma, or by direct intracranial implantation, caused experimental mice to form metastases. The existing injection methods include: 1. left ventricle injection, 2. common carotid artery injection, and 3. stereotaxic intracranial orthotopic injection. The left ventricle injection relies on experience or under the guidance of B-ultrasound, punctures the left ventricle through the intercostal space of the mouse, ...

AI Technical Summary

Problems solved by technology

However, the common carotid artery in mice is relatively thin, with a diameter of no more than 1mm. Even if an insulin needle (0.3mm in diameter) is used, the damage to the blood vessel caused by puncture is very significant, which often leads to massive bleeding after the needle is pulled out. Combining the use of hemostatic materials to compress hemostasis is helpful. It is good for hemostasis, but it also increases the incidence of carotid artery thrombosis, causes cerebral infarction and seizures in mice, and increases the mortality rate of the model. Its ischemic and hypoxic environment is also not conducive to the progression of tumors in the brain

There is another problem that cannot be ignored: the almost inevitable leakage of tumor cells during puncture injection or after pulling out the puncture needle will cause tumor cells to grow in the neck, invade the trachea, and increase the mortality of model mice

3. Although stereotaxic intracranial orthotopic injection can effectively deliver tumor cells into the brain tissue, it cannot simulate the pathophysiological process of tumor brain metastasis, but directly inoculates the tumor to grow in the brain tissue

Brain tissue damage caused by puncture can also have a certain impact on the experimental results

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