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Method for rapidly detecting total mercury content of urban relocation soil by using archaea molecular marker OTU69

A content and soil technology, applied in the fields of biochemical equipment and methods, soil material testing, material inspection products, etc., can solve problems such as direct pollution, unreasonable application of mercury-containing fertilizers and pesticides, and irrigation, etc., and achieve short time required. , to achieve rapid automatic detection, the effect of small sample demand

Active Publication Date: 2021-07-20
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mercury in soil mainly comes from: 1) soil parent material, mercury in soil parent material is the most basic source, and the content of mercury element in primary rock directly determines the content of mercury in soil; 2) atmospheric deposition, mercury in the atmosphere After entering the soil, most of them are quickly absorbed or fixed by clay minerals and organic matter in the soil, and enriched in the soil surface; 3) direct pollution, mainly including industrial production waste and urban domestic waste stacking, unreasonable application of mercury-containing fertilizers and pesticides and irrigation

Method used

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  • Method for rapidly detecting total mercury content of urban relocation soil by using archaea molecular marker OTU69
  • Method for rapidly detecting total mercury content of urban relocation soil by using archaea molecular marker OTU69
  • Method for rapidly detecting total mercury content of urban relocation soil by using archaea molecular marker OTU69

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, find the OUT relevant to the total mercury content of soil

[0068] 1. Collection of soil samples

[0069] In November 2017, research plots were set up in representative parks and green spaces in 16 administrative districts of Shanghai.

[0070] Sampling method: Soil sample collection follows the sampling principle of multi-point mixing. Select 8 sampling points for each plot, and use a 2.5cm diameter soil drill to collect 0-20cm surface soil respectively, and then mix them into 1 soil sample.

[0071] A total of 76 soil samples were collected.

[0072] The soil samples were mixed evenly and passed through a 2mm sieve to remove plant roots, gravel and other debris. Each soil sample was then divided into two. One sample was air-dried naturally, and then sampled for the determination of soil chemical properties in step 2; the other sample was stored at -80°C, and then sampled for the extraction of total soil DNA in step 3.

[0073] 2. Analysis and determ...

Embodiment 2

[0095] Embodiment 2, establishing the linear relationship between OTU and soil chemical characteristics

[0096] 1. Archaeal Marker Gene Probe Design

[0097] According to the sequencing results, a probe for detecting OTU69 was designed.

[0098] Probe69-1 probe (SEQ ID NO: 1 of the SEQUENCE LISTING): 5'-ACCCGCTCAACGGTTGGGCT-3'.

[0099] Probe69-2 probe: 5'-TGATGGGATGGCCTCGAGCT-3'.

[0100] The Probe69-1 probe is a Taqman probe with a fluorescent group FAM at the 5' end and a fluorescent quencher TAMRA at the 3' end. The Probe69-2 probe is a Taqman probe with a fluorescent group FAM at the 5' end and a fluorescent quencher TAMRA at the 3' end.

[0101] According to the sequencing results, a probe for detecting OTU384 was designed.

[0102] Probe384-1: 5'-TGAACAGGCTTAGTGCCTATT-3'.

[0103] Probe384-2: 5'-AGTGCCTATTCAGTGCCGCA-3'.

[0104] The Probe384-1 probe is a Taqman probe with a fluorescent group FAM at the 5' end and a fluorescent quencher TAMRA at the 3' end. The P...

Embodiment 3

[0127] Example 3, Molecular detection of total mercury content in unknown urban green space soil samples

[0128] In Shanghai, Nanjing, and Suzhou, three urban park green spaces were randomly selected.

[0129] Sampling method: Soil sample collection follows the sampling principle of multi-point mixing. Select 8 sampling points for each plot, and use a 2.5cm diameter soil drill to collect 0-20cm surface soil respectively, and then mix them into 1 soil sample.

[0130] The soil samples were mixed evenly and passed through a 2mm sieve to remove plant roots, gravel and other debris. Each soil sample was then divided into two. One sample was air-dried naturally, and then a sample was taken to detect the total mercury content, and the measured value of the total mercury content (in mg / kg) was obtained; the other sample was stored at -80°C, and then sampled for extraction of total soil DNA.

[0131] 1. Extract the total DNA from the soil sample. Using MoBio DNA extraction kit (...

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Abstract

The invention discloses a method for rapidly detecting the total mercury content of urban relocation soil by using an archaea molecular marker OTU69. The invention provides a DNA molecule (probe) as shown in a sequence 1 in a sequence table. The invention also protects the application of the probe in detection or auxiliary detection of the total mercury content of soil. The invention also protects the application of the probe in comparison of the total mercury content of soil in different plots. The method provided by the invention is used for detecting the total mercury content of the soil or comparing the total mercury content of the soil in different land parcels, and has the advantages of small sample demand, no need of pretreatment, short required time, low labor cost and capability of realizing rapid and automatic detection of a large batch of samples. The method has important application and popularization values for evaluation of soil samples.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for quickly detecting the total mercury content in soil of an urban relocation site by using an archaeal molecular marker OTU69. Background technique [0002] With the rapid development of urbanization, my country's cities, especially megacities, have entered the stage of urban development dominated by "urban redevelopment", and a large number of urban relocation sites have emerged, especially in cities in economically developed eastern coastal areas. Taking Shanghai as an example, 80%-90% of the construction green space within the built-up area is carried out on land such as the demolition of villages in the city and the relocation of old factories. Scientific and objective monitoring and evaluation of the soil quality is the basis for urban relocation. The premise and important reference basis for ecological restoration and landscaping. [0003] At present, the detection of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6851C12Q1/04C12N15/11
CPCC12Q1/689C12Q1/6851C12Q2531/113C12Q2561/113C12Q2561/101C12N15/11C12Q1/04G01N33/24C12Q1/6888C12Q2600/158
Inventor 张浪韩继刚张维维金荷仙王香春
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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