Application of pollen polysaccharide extracting solution in plant stress resistance

A pollen polysaccharide and plant stress resistance technology, which is applied in the agricultural field, can solve problems that have not been studied in the agricultural field, and achieve the effect of improving plant drought resistance and expanding application fields

Active Publication Date: 2021-07-20
CHENGDU NEWSUN CROPSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research reports on rapeseed pollen polysaccharides are in the fields of food and medicine, and there is no research in the field of agriculture

Method used

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  • Application of pollen polysaccharide extracting solution in plant stress resistance
  • Application of pollen polysaccharide extracting solution in plant stress resistance
  • Application of pollen polysaccharide extracting solution in plant stress resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Material preparation

[0041] Test name: Biological activity test of pollen polysaccharides improving drought resistance of wheat

[0042] Test object: Wheat (Yunong 495)

[0043] After the wheat seeds are sterilized by 5% sodium hypochlorite for 10 minutes, wash them with water for 5-6 times, wash off the sodium hypochlorite solution, soak the seeds overnight for 12 hours, put filter paper in the germination box, add a small amount of water to soak the filter paper, and spread the seeds on the filter paper Place them in an artificial climate box at 25°C to germinate for 1 day under the condition of avoiding light. Select wheat seeds with the same size, no pests and diseases, fullness, and the same degree of whiteness, and move them to hydroponic bottles. Put the mouth of the bottle into the planting basket Put 10 wheat seeds into it, and use it for experiment after hydroponics for 3 days.

[0044] 2. Application method

[0045] Configure 20% PEG solution, add 200...

Embodiment 2

[0057] 1. Material preparation

[0058] Test name: Biological activity test of pollen polysaccharides improving wheat resistance to salinity and alkalinity

[0059] Test object: Wheat (Yunong 495)

[0060] After the wheat seeds are sterilized by 5% sodium hypochlorite for 10 minutes, wash them with water for 5-6 times, wash off the sodium hypochlorite solution, soak the seeds overnight for 12 hours, put filter paper in the germination box, add a small amount of water to soak the filter paper, and spread the seeds on the filter paper Place them in an artificial climate box at 25°C to germinate for 1 day under the condition of avoiding light. Select wheat seeds with the same size, no pests and diseases, fullness, and the same degree of whiteness, and move them to hydroponic bottles. Put the mouth of the bottle into the planting basket Put 10 wheat seeds into it, and use it for experiment after hydroponics for 3 days.

[0061] 2. Application method

[0062] Configure saline-al...

Embodiment 3

[0074] 1. Material preparation

[0075] Test name: Biological activity test of pollen polysaccharides improving drought resistance of pepper seedlings

[0076] Test object: Pepper seedlings

[0077] Pepper seeds were sown in AOS, and transplanted into seedling pots when they grew to 4 leaves, one plant per pot, and used for experiments after recovery. Select pepper seedlings with similar growth and uniform watering to make the soil completely watered, and then no longer water.

[0078] 2. Application method

[0079] In this experiment, 6 treatment groups (a blank control and a positive control) were set up, with 6 plants in each treatment. When the potting soil is dry, the seedlings are sprayed according to different settings, and the amount of the liquid is appropriate to keep the plants moist (about 3mL / plant). The positive control treatment sprayed 80ppm of compound amino acid powder (40% powder, Chengdu Chelation Biotechnology Co., Ltd., provided by the manufacturer in...

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Abstract

The invention relates to application of a pollen polysaccharide extracting solution in plant stress resistance, and belongs to the technical field of agriculture. The technical problem to be solved by the invention is to provide new application of a pollen polysaccharide extracting solution, namely application of the pollen polysaccharide extracting solution in plant stress tolerance, and the pollen polysaccharide extracting solution is prepared by the following method: mixing rape pollen and water, heating, stirring and extracting for at least one time, filtering, merging filtrates, adding chitosan into the filtrates to obtain a solution to be clarified, maintaining the solution to be clarified at 60-80 DEG C for at least 1 hour, cooling, standing, and carrying out solid-liquid separation to obtain a liquid, namely the pollen polysaccharide extracting solution. The pollen polysaccharide extracting solution is obtained by adopting a method of water extraction and clarification, the pollen polysaccharide extracting solution has the effect of improving the stress resistance of plants, the application field of pollen polysaccharide is expanded, and meanwhile, a new choice is provided for the stress resistance of the plants.

Description

technical field [0001] The invention relates to the application of pollen polysaccharide extract in plant stress resistance, and belongs to the field of agricultural technology. Background technique [0002] Crops often encounter adversity stress during the growth process. Plant adversity refers to various environmental factors that are unfavorable to plant growth and survival, such as high temperature, drought, low temperature, salinity, etc. Under long-term stress conditions, crops will reduce yield or even die, causing damage to agricultural production. At the same time, these environmental stress factors also limit the distribution of some cultivated crops with good agronomic and horticultural traits. [0003] With the frequent occurrence of extreme climates in the world, plant growth regulators have become just-needed products in agricultural production. Rapeseed is the traditional main oil crop in my country, and it is planted in a wide area. Rapeseed pollen is the l...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P21/00
CPCA01N43/16
Inventor 何其明黄瑾李玉珠刘科王艳玲吴丹黄丹康亮珠杭林枫
Owner CHENGDU NEWSUN CROPSCI
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