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Receptor reagent and application thereof

A reagent and receptor technology, applied in the field of receptor reagents, can solve problems such as extremely high sensitivity requirements, and achieve the effects of high sensitivity, improved detection precision, and reduced production costs

Active Publication Date: 2021-07-16
BEYOND DIAGNOSTICS (SHANGHAI) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the progress of the detection industry, there is an increasing demand for ultrasensitive reagents, which not only require extremely high sensitivity, but also require a very wide detection range. It is difficult for the existing homogeneous chemiluminescence detection methods to meet the above detection requirements. condition

Method used

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  • Receptor reagent and application thereof
  • Receptor reagent and application thereof
  • Receptor reagent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: acceptor particle a preparation

[0060] 1.1 Preparation and characterization process of carrier

[0061]1. Prepare a 100ml three-necked flask, add 40mmol styrene, 5mmol acrolein, and 10ml water, stir for 10min, and pass N 2 30min;

[0062] 2. Weigh 0.11g of ammonium persulfate and 0.2g of sodium chloride, dissolve them in 40ml of water to form an aqueous solution. This aqueous solution is added in the reaction system of step 1, continues to pass through N 2 30min;

[0063] 3. Raise the temperature of the reaction system to 70°C and react for 15 hours;

[0064] 4. Cool the emulsion after the reaction to room temperature and filter it with a suitable filter cloth. The obtained emulsion is washed with deionized water by centrifugation and sedimentation until the conductivity of the supernatant at the beginning of centrifugation is close to that of deionized water, then diluted with water, and preserved in the form of emulsion;

[0065] 5. The Gauss...

Embodiment 2

[0088] Embodiment 2: preparation of acceptor particle b

[0089] 2.1 Preparation and characterization process of carrier

[0090] 1. Prepare a 100ml three-necked flask, add 40mmol styrene, 5mmol acrolein, and 10ml water, stir for 10min, and pass N 2 30min;

[0091] 2. Weigh 0.11g of ammonium persulfate and 0.2g of sodium chloride, dissolve them in 40ml of water to form an aqueous solution. This aqueous solution is added in the reaction system of step 1, continues to pass through N 2 30min;

[0092] 3. Raise the temperature of the reaction system to 70°C and react for 15 hours;

[0093] 4. Cool the emulsion after the reaction to room temperature and filter it with a suitable filter cloth. The obtained emulsion is washed with deionized water by centrifugation and sedimentation until the conductivity of the supernatant at the beginning of centrifugation is close to that of deionized water, then diluted with water, and preserved in the form of emulsion;

[0094] 5. The Gau...

Embodiment 3

[0107] Embodiment 3: Utilize anthrone method to detect the sugar content of microsphere

[0108] 1. Microsphere sample pretreatment:

[0109] Take the donor reagent A containing 1 mg of donor microspheres a in Example 1 and the donor reagent B containing 1 mg of donor microspheres b in Example 2 respectively, centrifuge at 20000 g for 40 min, pour off the supernatant and use purified water to sonicate Disperse, repeat centrifugation and disperse three times, then dilute to 1 mg / mL with purified water.

[0110] 2. Preparation of glucose standard solution:

[0111] Prepare 1mg / mL glucose stock solution with purified water to prepare standard solution curves of 0mg / mL, 0.025mg / mL, 0.05mg / mL, 0.075mg / mL, 0.10mg / mL, 0.15mg / mL, such as figure 1 shown.

[0112] 3. Preparation of anthrone solution: use 80% sulfuric acid solution to prepare 2 mg / mL (this solution is stable within 24 hours at room temperature, and it is ready for use).

[0113] 4. Add 0.1mL glucose standard solution...

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Abstract

The invention discloses a receptor reagent and application thereof. The receptor reagent comprises a first buffer solution and receptor particles which suspend in the first buffer solution and can react with active oxygen to generate chemiluminescence signals, wherein the surfaces of the receptor particles are combined with biomolecules, and a particle size distribution variable coefficient C.V value of the receptor particles in the receptor reagent is not lower than 5% and not higher than 20%; and the sugar content in each milligram of the receptor particles is not higher than 40 [mu] g. The receptor reagent has the beneficial effects that the particle size distribution variable coefficient C.V value of the receptor particles in the receptor reagent is not lower than 5% and not higher than 20%; furthermore, the receptor reagent not only can meet the commercial requirement of mass production, but also has ultrahigh sensitivity and very wide detection range. Meanwhile, the sugar content in the receptor particles is controlled, so that the influence of sugar on a detection signal is reduced, the detection precision is improved, and the production cost is further reduced.

Description

technical field [0001] The technical solution relates to the field of chemiluminescence detection, more specifically, the technical solution relates to an acceptor reagent and its application. Background technique [0002] After more than half a century of development, immunoassays have developed many types. According to whether the substance to be tested is separated from the reaction system during the determination process, it can be divided into heterogeneous (Heterogenous) immunoassay and homogeneous (Homogeneous) immunoassay. Heterogeneous immunoassay refers to the operation process of introducing probes for labeling. Various related reagents need to be separated after mixing and reacting. The analyte is separated from the reaction system and then detected. It is the mainstream method in immunoassay. . Such as the well-known enzyme-linked immunosorbent assay (ELISA) and magnetic particle chemiluminescence. Homogeneous immunoassay refers to the direct measurement afte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N21/76
CPCG01N33/56983G01N21/76G01N2333/02
Inventor 康蔡俊陈义旺李临
Owner BEYOND DIAGNOSTICS (SHANGHAI) CO LTD
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